The effect of the layer structure on the activity of immobilized enzymes in ultrathin films

Caseli, Luciano; Santos, David S. dos; Foschini, Maurício; Gonçalves, Débora; Oliveira, Osvaldo N.

doi:10.1016/j.jcis.2006.07.013

Cited by 45 publications

(50 citation statements)

References 42 publications

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“…The LbL method has recently received much attention in immobilizing biomolecules especially for biosensor applications [13,14] and biocatalysis [15]. To our best knowledge, there are only few reports dealing with enzyme immobilization on membranes as support using LbL assembly.…”

Section: Introductionmentioning

confidence: 99%

Effect of enzyme location on activity and stability of trypsin and urease immobilized on porous membranes by using layer-by-layer self-assembly of polyelectrolyte

Guedidi

Yürekli

Deratani

et al. 2010

Journal of Membrane Science

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Section: Introductionmentioning

confidence: 99%

Effect of enzyme location on activity and stability of trypsin and urease immobilized on porous membranes by using layer-by-layer self-assembly of polyelectrolyte

Guedidi

Yürekli

Deratani

et al. 2010

Journal of Membrane Science

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“…185,186 In other instances, it is better to keep the enzyme only on the topmost layer because the main reactions occur on the surface. 47,187 Also worth mentioning is a nanoscale protein chip prepared with an etched polystyrene (PS) template, immobilized as LB films, used for an immunoassay exploring SERS spectra. 188 …”

Section: Graphenementioning

confidence: 99%

Nanomaterials for Diagnosis: Challenges and Applications in Smart Devices Based on Molecular Recognition

Oliveira

Iost

Siqueira

et al. 2014

ACS Appl. Mater. Interfaces

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156

137

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Clinical diagnosis has always been dependent on the efficient immobilization of biomolecules in solid matrices with preserved activity, but significant developments have taken place in recent years with the increasing control of molecular architecture in organized films. Of particular importance is the synergy achieved with distinct materials such as nanoparticles, antibodies, enzymes, and other nanostructures, forming structures organized on the nanoscale. In this review, emphasis will be placed on nanomaterials for biosensing based on molecular recognition, where the recognition element may be an enzyme, DNA, RNA, catalytic antibody, aptamer, and labeled biomolecule. All of these elements may be assembled in nanostructured films, whose layer-by-layer nature is essential for combining different properties in the same device. Sensing can be done with a number of optical, electrical, and electrochemical methods, which may also rely on nanostructures for enhanced performance, as is the case of reporting nanoparticles in bioelectronics devices. The successful design of such devices requires investigation of interface properties of functionalized surfaces, for which a variety of experimental and theoretical methods have been used. Because diagnosis involves the acquisition of large amounts of data, statistical and computational methods are now in widespread use, and one may envisage an integrated expert system where information from different sources may be mined to generate the diagnostics.

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“…Estos estudios han analizado la interacción de los polímeros durante la formación de las películas, así como el efecto de la distribución e interacciones de los compuestos sobre las propiedades físicas, antimicrobianas, químicas, entre otras [59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74]. Muchas aplicaciones han sido desarrolladas en campos de la investigación como en el área de electrónica [75][76][77][78][79][80][81][82][83][84][85], la inmovilización de enzimas [86][87][88][89], el desarrollo de nuevos materiales con propiedades ópticas específicas [81,[90][91][92], la liberación de compuestos [93][94][95], entre otras.…”

Section: Conformación De Películas En Multicapasunclassified

Modificaciones físicas, químicas y enzimáticas y sus efectos sobre las propiedades de las películas de quitosano

Valderrama

Albarracín

2014

Matéria (Rio J.)

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Modificaciones físicas, químicas y enzimáticas y sus efectos sobre las propiedades de las películas de quitosano.Physical, chemical and enzymatic modifications and their effects on the properties of chitosan films. RESUMENLas recientes investigaciones en el campo de biopolímeros han intentado brindar soluciones tecnológicas a los retos en el campo de la ciencia y la tecnología de los materiales. En los últimos años, se han llevado a cabo investigaciones con el objetivo de modificar las propiedades de películas empleando tratamientos enzimáticos y físicos, tales como la aplicación de radiaciones, pulsos eléctricos, plasma, tratamientos térmicos, uso de fluidos supercríticos, así como la conformación de películas en multicapas. Estas modificaciones cambian las propiedades antimicrobianas, físicas y químicas de las películas de quitosano y permiten desarrollar una extensa cantidad de aplicaciones en los campos de biomedicina, farmacología, biotecnología y en la industria de cosméticos y de alimentos. El objetivo del presente artículo de revisión es dar a conocer los nuevos avances en el campo de modificaciones físicas y enzimáticas de películas de quitosano explicando diferentes técnicas empleadas en los últimos años.Palabras clave: quitosano, polímeros, películas modificadas, aditivos alimenticios. ABSTRACTRecent research on biopolymer has focused its efforts to provide technological solutions to current and future challenges in the field of science and technology of materials. The properties of chitosan films have been modified by enzymatic and physical treatments such as the application of radiation, electric pulses, plasma, heat treatments, use of supercritical fluids as well as the formation of multilayer films. These modifications change antimicrobial, physical and chemical properties of chitosan films, and allow developing a wide range of applications in the fields of biomedicine, pharmacology, biotechnology, cosmetic and food industries. The purpose of this review is to present new developments in the field of physical and enzymatic modifications of chitosan films describing the different techniques carried out on the last years.

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The effect of the layer structure on the activity of immobilized enzymes in ultrathin films

Cited by 45 publications

References 42 publications

Effect of enzyme location on activity and stability of trypsin and urease immobilized on porous membranes by using layer-by-layer self-assembly of polyelectrolyte

Effect of enzyme location on activity and stability of trypsin and urease immobilized on porous membranes by using layer-by-layer self-assembly of polyelectrolyte

Nanomaterials for Diagnosis: Challenges and Applications in Smart Devices Based on Molecular Recognition

Modificaciones físicas, químicas y enzimáticas y sus efectos sobre las propiedades de las películas de quitosano

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