The effect of time between venipuncture, processing and freezing on the measurement of coagulation factor levels

Engbers, M. J.; Cushman, Mary; Rosendaal, F. R.; Vlieg, Astrid van Hylckama

doi:10.1111/j.1538-7836.2012.04792.x

Cited by 2 publications

(3 citation statements)

References 14 publications

(27 reference statements)

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“…Explanations for poor correlation between ideal and REGARDS-processed samples include incomplete removal of platelets from REGARDS-processed specimens in the remote centrifugation, longer time to centrifugation in REGARDS samples [4], temperature variations in REGARDS-processed samples [5], low variability of the biomarker between subjects, or the CV of the assay.…”

Section: Resultsmentioning

confidence: 99%

“…This allowed recruitment of a nationally representative sample. While standardized training and methods were used, since field processing and sample shipping may affect the quality of samples [1–5], the validity of assays run on these samples required investigation. Here, we provide results on analyte validity using split samples from 20 volunteers, and results on sample yield and quality from >30,000 REGARDS participants from a baseline in-home visit.…”

Section: Introductionmentioning

confidence: 99%

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Validating laboratory results in a national observational cohort study without field centers: The Reasons for Geographic and Racial Differences in Stroke cohort

Gillett

Boyle

Zakai

et al. 2014

Clinical Biochemistry

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Objectives The REasons for Geographic and Racial Differences in Stroke (REGARDS) study is a prospective cohort of 30,239 Americans in the contiguous United States; the first of this scale to use home visits to obtain, process, and ship biologic samples to a core laboratory. Pre-analytical factors resulting from this study design may affect the results of some laboratory assays. We investigated the impact of REGARDS processing on a variety of analytes. Design and methods In REGARDS, blood samples were processed in the field by technicians who were trained on standardized methods for phlebotomy and sample processing. Field processing included centrifugation using varying non-uniform equipment and shipping overnight on ice to the University of Vermont, where samples were re-centrifuged for 30,000 ×g-minutes and stored at −80 °C. We assessed the effects of REGARDS sample handling by processing split samples from 20 volunteers using either ideal procedures or simulated REGARDS procedures. Assays for 19 analytes for potential study in REGARDS were then run on both samples and results compared. Results Spearman correlation coefficients for analytes measured in ideal versus REGARDS processed samples ranged from 0.11 to 1.0. Thirteen of 19 analytes were highly correlated (>0.75), but platelet proteins were more variable. Conclusions Simulation of non-optimal field processing and shipment to a central laboratory showed high variability in analytes released by platelets. The majority of other analytes produced valid results, but platelet contamination in REGARDS samples makes measurement of platelet proteins unadvisable in these samples. Future analytes considered by REGARDS or similar studies should undergo similar pilot testing.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Validating laboratory results in a national observational cohort study without field centers: The Reasons for Geographic and Racial Differences in Stroke cohort

Gillett

Boyle

Zakai

et al. 2014

Clinical Biochemistry

View full text Add to dashboard Cite

show abstract

“…In contrast, Lamboo et al reported no significant changes in PT, APTT, FV, and fibrinogen during storage at room temperature for up to 6 h [24]. This might possibly be due to cold activation of FVIII and vWF Ag during the initial 6 h of storage at temperature 2-6°C (Group A) as compared to storage at room temperature (Group B) [25].…”

Section: Discussionmentioning

confidence: 91%

Coagulation Factor Activities Changes Over 5 Days in Thawed Fresh Frozen Plasma Stored at Different Initial Storage Temperatures

Noordin

Karim²,

Mohammad

et al. 2017

Indian J Hematol Blood Transfus

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Thawed plasma is fresh frozen plasma (FFP) that has been stored for 5 days at 1-6°C. Duration of storage and different storage temperatures might affect the coagulation factor activity in thawed FFP. This study measured the changes of coagulation factor activities over 5 days in thawed FFP and stored at two different initial storage temperatures. Thirty-six units of FFP, which consisted of nine units each from blood groups A, B, AB, and O, were thawed at 37°C. Each unit was divided into two separate groups (Group A and Group B) based on initial storage temperature. The first group was stored at 2-6°C for 5 days (Group A). The second group was stored at 20-24°C for initial 6 h followed by 2-6°C for 5 days (Group B). Prothrombin time (PT), activated partial thromboplastin time (APTT), coagulation factor activities of fibrinogen, factor (F) II, FV, FVII, FVIII, FIX, FX, and von Willebrand factor antigen (vWF Ag) were assessed at baseline after thawing, at 6 h, and on days 1, 3, and 5 of storage for both groups. All coagulation factors mean activities in both storage groups decreased significantly over 5 days of storage. The mean FVIII activity at day 5 of storage was 36.9% in Group A and 39.8% in Group B. The other coagulation factors mean activities were [ 50% on day 5 of storage in both groups. The coagulation factor activities of thawed FFP stored for 5 consecutive days were reduced in the two storage groups but most of the activities were still above 30%. This study suggests that thawed FFP stored for 5 days has the potential to ameliorate coagulation factor deficiencies in affected patients.

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The effect of time between venipuncture, processing and freezing on the measurement of coagulation factor levels

Cited by 2 publications

References 14 publications

Validating laboratory results in a national observational cohort study without field centers: The Reasons for Geographic and Racial Differences in Stroke cohort

Validating laboratory results in a national observational cohort study without field centers: The Reasons for Geographic and Racial Differences in Stroke cohort

Coagulation Factor Activities Changes Over 5 Days in Thawed Fresh Frozen Plasma Stored at Different Initial Storage Temperatures

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