The effect of titanium dioxide nanoparticles on antioxidant gene expression in tilapia (Oreochromis niloticus)

Varela-Valencia, Ruth; Gómez-Ortíz, N.M.; Oskam, Gerko; Coss, Romeo de; Rubio-Piña, Jorge; Río-García, Marcela Del; Albores-Medina, Arnulfo; Zapata-Pérez, Omar

doi:10.1007/s11051-014-2369-3

Cited by 29 publications

(14 citation statements)

References 62 publications

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“…The increase in GST activity, in turn, may be due to the increase in the biotransformation process of GSH and NP conjugation. A similar effect of TiO 2 ‐NP was found in tilapias, whose liver increased GST expression. In fish, hepatic GST is known to metabolize xenobiotics, such as metals, and lipid hydroperoxides, evidencing its important role in defending the organism against oxidative damage .…”

Section: Discussionsupporting

confidence: 56%

Overview of the toxic effects of titanium dioxide nanoparticles in blood, liver, muscles, and brain of a Neotropical detritivorous fish

Carmo

Siqueira

Azevedo

et al. 2019

Environmental Toxicology

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The toxicity of titanium dioxide nanoparticles (TiO 2 -NP) in the blood, liver, muscle, and brain of a Neotropical detritivorous fish, Prochilodus lineatus, was tested. Juvenile fish were exposed to 0, 1, 5, 10, and 50 mg L −1 of TiO 2 -NP for 48 hours (acute exposure) or 14 days (subchronic exposure) to evaluate changes in hematology, red blood cell (RBC) genotoxicity/mutagenicity, liver function (reactive oxygen species (ROS) production, antioxidant responses, detoxification, and histopathology), acetylcholinesterase (AChE) activity in muscles and brain, and Ti bioaccumulation. TiO 2 -NP did not cause genetic damage to RBC, but acutely decreased white blood cells (WBC) and increased monocytes. Subchronically, RBC decreased, mean cell volume and hemoglobin increased, and WBC and lymphocytes decreased. Therefore, NP has the potential to affect immune system and increase energy expenditure, reducing the fish's ability to avoid predator and to resist pathogens. In the liver, acute exposure decreased ROS and increased glutathione (GSH) content, while subchronic exposure decreased superoxide dismutase activity and increased glutathione-S-transferase (GST) activity and GSH content. GSH and GST seem to play an essential role in metabolizing NP and ROS, likely increasing hepatocytes' metabolic rate, which may be the cause of observed cell hypertrophy, disarrangement of hepatic cords and degenerative morphological alterations. Although most studies indicate that the kidney is responsible for metabolizing and/or eliminating TiO 2 -NP, this study shows that the liver also has a main role in these processes. Nevertheless, Ti still accumulated in the liver, muscle, and brain and decreased muscular AChE activity after acute exposure, showing neurotoxic potential.More studies are needed to better understand the biochemical pathways TiO 2 -NP are metabolized and how its bioaccumulation may affect fish homeostasis and survival in the environment. K E Y W O R D S bioaccumulation, glutathione-S-transferase neurotoxicity, immune system, reactive oxygen species

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Section: Discussionsupporting

confidence: 56%

Overview of the toxic effects of titanium dioxide nanoparticles in blood, liver, muscles, and brain of a Neotropical detritivorous fish

Carmo

Siqueira

Azevedo

et al. 2019

Environmental Toxicology

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“…Similar low toxicity of TiO 2 -NPs was also observed in Oncorhynchus mykiss, Piaractus mesopotamicus, and Carassius auratus with no or minor impact at an exposure concentration of 100 mg/L (Warheit et al, 2007;Ates et al, 2013;Clemente et al, 2013). However, direct intraperitoneal injection of 1 ml (1 mg/L) significantly increased the antioxidant genes (e.g., CAT, GST, and SOD) expression within 6 h (Varela-Valencia et al, 2014), indicating the low bioavailability of waterborne TiO 2 -NPs as a possible reason for observed low toxicity.…”

Section: Fishsupporting

confidence: 65%

Significance of physicochemical and uptake kinetics in controlling the toxicity of metallic nanomaterials to aquatic organisms

Wang

2014

J. Zhejiang Univ. Sci. A

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With the extensive applications of metallic-based nanomaterials (MNs), concerns are growing of their potential impact on aquatic organisms. Unlike traditional metal pollutants, MNs have different surface properties and compositions, which may modify their impact on aquatic environments as well as their bioavailability to aquatic organisms. Kinetic processes of MNs, such as dissolution, stabilization, aggregation, and sedimentation, are important in determining their bioavailability and subsequent toxicity to aquatic organisms. Among all of the physicochemical kinetics, the dissolution of MNs attracts the most attention, due to their potential toxicity generated by dissolved ions. This review summarizes the dissolution behavior of three common MNs, i.e., ZnO nanoparticles (ZnO-NPs), Ag nanoparticles (Ag-NPs), and TiO 2 nanoparticles (TiO 2-NPs), in toxicological studies. A kinetic model was developed to evaluate the contribution of dissolved ion on the total MN accumulation. Finally, toxicological data of the MNs to algae, zooplankton, and fish are summarized and interpreted based on their kinetics. Different dissolution rates were observed for ZnO-NPs, Ag-NPs, and TiO 2-NPs, and their solubility also varied during different toxicological studies, leading to a variable but increasing waterborne ion concentration during exposure. The bioavailability of these MNs and corresponding ions also varied for different aquatic organisms (e.g., algae, zooplankton, and fish). Specifically, the MNs appeared to be more bioavailable to daphnids, rendering a minor contribution of ion during short-term exposure. Generally, dissolved ion contributed partially to toxicity of ZnO-NPs and Ag-NPs, while the toxicity of TiO 2-NPs was mainly due to the generated reactive oxygen species (ROS). Additionally, the role of dissolved ion in both MN bioaccumulation and toxicity intensified during chronic exposure as a result of dissolution, thus it is critical to monitor the dissolution of MNs in toxicological studies. This review emphasizes the importance of integrating physicochemical kinetics and uptake kinetics in evaluating the bioavailability and toxicity of both MNs and dissolved ions.

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“…ROS generation was found to have a direct correlation with the decrement in the chlorophyll con- tent for both anatase and rutile NP-treated cells. Varela-Valencia et al (2014) noted that the physicochemical characteristics of TiO 2 NPs varied the CAT, GST, and SOD transcript levels in O. niloticus, which were treated with the NPs. They also reported that ROS generation was higher for the rutile phase than that of anatase phase.…”

Section: Oxidative Stress Assessmentmentioning

confidence: 99%

Combined toxicity of two crystalline phases (anatase and rutile) of Titania nanoparticles towards freshwater microalgae: Chlorella sp

et al. 2015

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The effect of titanium dioxide nanoparticles on antioxidant gene expression in tilapia (Oreochromis niloticus)

Cited by 29 publications

References 62 publications

Overview of the toxic effects of titanium dioxide nanoparticles in blood, liver, muscles, and brain of a Neotropical detritivorous fish

Overview of the toxic effects of titanium dioxide nanoparticles in blood, liver, muscles, and brain of a Neotropical detritivorous fish

Significance of physicochemical and uptake kinetics in controlling the toxicity of metallic nanomaterials to aquatic organisms

Combined toxicity of two crystalline phases (anatase and rutile) of Titania nanoparticles towards freshwater microalgae: Chlorella sp

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