The effect of training and the Alka competition on oxidative stress and metabolic parameters in two horse breeds

Abstract

The aim of this study was to investigate the effects of training and the Alka competition upon the activity/ concentrations of antioxidative and muscle enzymes and metabolic parameters, as well as their correlations, in the serum of Thoroughbred (TH) and Croatian Warmblood (CWH) horses. The experiment was carried out on nine THs and seven CWHs. Blood samples were collected 30, 21 and 14 days before the Alka competition, immediately after the competition, and 7 days after. Serum concentrations of malondialdehyde (MDA), the activity of glutathione peroxidase (GSH-Px), total superoxide dismutase (SOD), paraoxonase 1 (PON 1), creatine kinase (CK), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were determined, as well as glucose, triglyceride, nonesterified fatty acids (NEFA), total cholesterol and high density lipoprotein cholesterol (HDL-C) concentrations. Serum MDA concentrations in both breeds were significantly higher 30 days before the Alka than immediately after the race. Serum activity of SOD in TH was significantly higher 21 days before the Alka compared to the values after 7 days of rest. Serum NEFA concentration in TH was

Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide

“…Physical exercise can cause oxidative stress. Several studies showed that the pathogenesis of exercise-induced myopathies and inflammation in horses may be related the reactive oxidative species (ROS) generated during different kinds of effort [16,17]. ROS may damage not only the basic cellular structure but may also cause DNA damage [18].…”