The effect of vitamin E supplementation on reduction of lymphocyte DNA damage induced by T-2 toxin and deoxynivalenol in weaned pigs

Frankič, Tamara; Salobir, Janez; Rezar, Vida

doi:10.1016/j.anifeedsci.2007.06.012

Cited by 56 publications

(35 citation statements)

References 40 publications

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“…Although DON did not increase MDA in the plasma or liver in the above-cited study, the oxidative stress pathway was suggested as a plausible mechanism for the observed DNA damage. Similarly, DNA damage was reported by Frankic et al (2008) in pigs (4 mg DON/kg of feed for 14 days) and Awad et al (2012) in chicken (10 mg DON/kg of feed for 35 days). According to the literature cited DON is considered to have negligible oxidative stress inducing effect and thus, it is supposed that DON is directly genotoxic or acts via different epigenetic mechanisms (e.g.…”

Section: Discussionmentioning

confidence: 54%

Subchronic exposure to deoxynivalenol exerts slight effect on the immune system and liver morphology of growing rabbits

et al. 2017

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As the most common grain contaminant worldwide, deoxynivalenol is of high importance despite its low toxicity compared to other trichothecene mycotoxins. Data on the effects of deoxynivalenol in rabbits are scarce. Thus, the aim of this study was to investigate the effects of dietary deoxynivalenol fed at a high level (10 mg/kg of feed) on the productive performance, blood indices, immunological variables, histopathological changes, and genotoxicity in rabbits. Forty-eight Pannon White rabbits were exposed to contaminated diets for three weeks. Despite its high concentration, deoxynivalenol did not affect the feed intake, body weight, and body weight gain. Liver and kidney function was not affected, as shown by the clinical chemistry indices. Conversely, in two rabbits the toxin caused mild fibrosis of the liver, without degenerative changes of the hepatocytes. No genotoxicity could be observed either. Gut cytokines and the phagocytic activity of the macrophages did not differ significantly. The percentage of neutrophils was significantly lower, whereas that of eosinophils was significantly higher in the toxin-fed group. Deoxynivalenol did not cause significant changes in gut and villus morphology. In 4 out of the 6 deoxynivalenol-treated animals, the ratio of lymphoblast proliferation and simultaneous apoptosis shifted towards apoptosis in the gut-associated lymphoid tissue. In the central part of the lymphoid follicles of the spleen, lymphocyte depletion and follicular atrophy could be detected. It can be concluded that rabbits are less sensitive to deoxynivalenol, but the findings confirm that this Fusarium toxin is capable of modulating the immune response. Mycotoxin, immune response, histology, blood indicesMycotoxins are secondary metabolites of filamentous fungi occurring worldwide. Despite being the least toxic trichothecene, the ubiquitous occurrence of deoxynivalenol (DON) in grains increases its importance for food and feed safety. The pig is the most sensitive, whereas ruminants are the least sensitive animal species to DON (Pestka and Smolinski 2005;Pestka 2007;Sobrova et al. 2010). Monogastric animals are extremely prone to growth and body weight gain suppression upon DON exposure. Deoxynivalenol possesses immunomodulatory properties as well (Pestka and Smolinski 2005). To the best of our knowledge, only few experiments have been conducted with DON in rabbits (Khera et al. 1986;Hewitt et al. 2012). The aim of this study, therefore, was to investigate the effects of 10 ppm (mg/kg feed) of dietary DON which is twice as much as the highest guidance value

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Section: Discussionmentioning

confidence: 54%

Subchronic exposure to deoxynivalenol exerts slight effect on the immune system and liver morphology of growing rabbits

et al. 2017

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“…Diet supplementation with tomato paste, vitamin E and their combination decreases significantly DNA fragmentation in liver tissue of Cd-exposed fishes. Vitamin E was reported by many authors to be effective in this concern (Cadenas and Barja 1999;Frankic et al 2008). Elkomy and Hassan (2005) emphasized on the role of tomato juice as a DNA-protective agent against thioacetamide hepatotoxicity in male rat.…”

Section: Discussionmentioning

confidence: 99%

Effects of cadmium on some haematological and biochemical characteristics of Oreochromis niloticus (Linnaeus, 1758) dietary supplemented with tomato paste and vitamin E

Mekkawy

Mahmoud

Wassif

et al. 2010

Fish Physiol Biochem

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The present study investigates the potential protective effects of tomato paste (9 mg/kg-lycopene) in comparison with vitamin E (50 mg/kg) against the impacts of cadmium (Cd) toxicity (4.64 mg/l: ¼ of 96 h LC50) on fishes Cd exposed for 15 and 30 days. Cd impacts were evaluated in terms of biological, haematological and biochemical characteristics. Cd significantly induced free radicals in serum and liver. The activities of aspartate aminotransferase and alanine aminotransferase in serum were significantly increased due to Cd. Treatment with Cd caused a significant increase in Lipid peroxidation and DNA fragmentation in liver tissue and serum glucose and total lipid. On the other hand, Cd significantly led to decline in serum total protein, blood haemoglobin, red blood cell count, haematocrit value, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. Dietary supplementation with vitamin E and/or tomato paste to Cd-exposed fish declined significantly the increased lipid peroxidation and DNA fragmentation in liver tissue and the increased aspartate aminotransferase, alanine aminotransferase, glucose and total lipid in serum to the normal condition. This supplementation also significantly increased the declined serum total protein, blood haemoglobin, red blood cell count, haematocrit value, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration to the normal state. Cd impacts and tomato paste/or vitamin E supplementations did not reflected on the condition factor of the fish. These findings demonstrated the beneficial diet supplementation of tomato paste phytonutrients and vitamin E in counteracting the harmful effects of Cd on the characters investigated.

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“…by Kruszewski et al (2008). The work of Frankič et al (2008) in an in vivo study showed that DON and T-2 toxin induced DNA damage in pig lymphocytes as measured by the comet assay, after feeding two groups of animals with feeds containing 4 and 3 mg/kg toxin, respectively. Similar results were obtained in experiments with chickens (Frankič et al, 2006;Sokolovic et al, 2008).…”

Section: Acta Veterinaria Hungarica 61 2013mentioning

confidence: 99%

Dose-related genotoxic effect of T-2 toxin measured by comet assay using peripheral blood mononuclear cells of healthy pigs

Horvatovich

Hafner

Bodnár

et al. 2013

Acta Veterinaria Hungarica

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T-2 toxin is the most acutely toxic trichothecene mycotoxin: it inhibits protein, DNA and RNA synthesis. The main goal of this study was to evaluate the rate of DNA damage caused by T-2 toxin in porcine mononuclear cells in increasing concentrations (0.1, 0.5 and 1.0 µmol) and after two different incubation periods (24 and 42 h). The lowest concentration caused DNA damage and about 50% of the treated cells could be categorised as having 1 to 4 scores in comet assay. In parallel with the increase of T-2 toxin concentration, the frequency of intact lymphocytes decreased from 50.2% (0.1 μM) to 36.3% (1.0 μM) in the first 24 h. In case of score 3, the highest concentration of T-2 toxin resulted in a 5-fold change, as compared to the lowest dose. Cells with score 4 were found only after exposure to 1.0 μM T-2 toxin. The exposure time did not have a significant effect on the results, while concentration did (P < 0.0001). However, a significant interaction between concentration and time as fixed factors (P < 0.0001) was found. When these were combined as a single factor, the results showed a significant toxin treatment effect on the results. It was concluded that a time-and dose-dependent DNA damaging effect of T-2 toxin could be demonstrated using peripheral blood mononuclear cells from healthy pigs by comet assay.

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The effect of vitamin E supplementation on reduction of lymphocyte DNA damage induced by T-2 toxin and deoxynivalenol in weaned pigs

Cited by 56 publications

References 40 publications

Subchronic exposure to deoxynivalenol exerts slight effect on the immune system and liver morphology of growing rabbits

Subchronic exposure to deoxynivalenol exerts slight effect on the immune system and liver morphology of growing rabbits

Effects of cadmium on some haematological and biochemical characteristics of Oreochromis niloticus (Linnaeus, 1758) dietary supplemented with tomato paste and vitamin E

Dose-related genotoxic effect of T-2 toxin measured by comet assay using peripheral blood mononuclear cells of healthy pigs

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