The effect of Zinc Oxide nanoparticles on Pseudomonas aeruginosa biofilm formation and virulence genes expression

Abdelraheem, Wedad M.; Mohamed, Ebtisam S.

doi:10.3855/jidc.13958

Cited by 22 publications

(10 citation statements)

References 21 publications

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“…We found that the expression of the QS-regulated genes lasI , lasR , rhlI , rhlR , pqsR , and pqsA decreased following exposure to ZnO NPs at concentrations of 900 µg/ml. Abdelraheem and Mohamed 55 found that ZnO NPs greatly down-regulated biofilm and virulence gene expression in PA clinical isolates for all genes studied aside from the toxA gene, which was up-regulated. The fold change reduction in the quorum sensing genes ( LasR , rhlI , and pqsR ) following exposure to ZnO NPs were 10.4-, 6.3-, and 8.7-fold, respectively 55 .…”

Section: Discussionmentioning

confidence: 96%

“…Abdelraheem and Mohamed 55 found that ZnO NPs greatly down-regulated biofilm and virulence gene expression in PA clinical isolates for all genes studied aside from the toxA gene, which was up-regulated. The fold change reduction in the quorum sensing genes ( LasR , rhlI , and pqsR ) following exposure to ZnO NPs were 10.4-, 6.3-, and 8.7-fold, respectively 55 . The impact of ZnO NPs is possibly similar to that of silver NPs as both reduce the expression of lasR and rhlR , causing a disturbance in QS circuits and an ensuing decline in biofilm formation 56 .…”

Section: Discussionmentioning

confidence: 96%

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The impact of biosynthesized ZnO nanoparticles from Olea europaea (Common Olive) on Pseudomonas aeruginosa growth and biofilm formation

Al-Momani

Balawi

Hamed

et al. 2023

Sci Rep

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There is a limitation in the range of effectual antibiotics due to the Pseudomonas aeruginosa (PA) infection due to its innate antimicrobial resistance. Researchers have therefore been concentrating their efforts to discover advanced and cost effective antibacterial agents among the ever-increasing PA bacterial resistance strains. It has been discovered that various nanoparticles can be employed as antimicrobial agents. Here, we evaluated the antibacterial properties of the Zinc Oxide nanoparticles (ZnO NPs), which was biosynthesized, being examined on six hospital strains of PA alongside a reference strain (ATCC 27853). A chemical approach was applied to biosynthesize the ZnO NPs from Olea europaea was performed, and confirmed by using X-ray diffraction and Scanning Electron Microscopes. The nanoparticles then applied their antibacterial properties to examine them against six clinically isolated PA strains alongside the reference strain. This process tested for the results of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The Growth, biofilm formation and eradication were analyzed. The influence of the differentiating degrees ZnO NPs in regard to Quorom sensing gene expression were further examined. The ZnO NPs exhibited a crystalline size and diameter (Dc) of 40–60 nm and both the MIC and MBC tests revealed positive outcomes of concentrations of 3 and 6 mg/ml for each PA strain, respectively. At sub inhibitory concentration, The ZnO NPs were found to significantly inhibit the growth and biofilm formation of all PA strains and decreases in the biomass and metabolic behavior of PA established biofilms; these decreases varied depending on the dosage. At ZnO NPs concentrations of 900 µg/ml, the expression of majority of quorum sensing genes of all strains were significantly reduced, at ZnO NPs concentrations of 300 µg/ml, few genes were significantly impacted. In conclusion, the treatment of PA and could be other antibiotic resistant bacteria can therefore be approached by using ZnO NPs as it has been uncovered that they withhold advanced antibacterial properties.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

The impact of biosynthesized ZnO nanoparticles from Olea europaea (Common Olive) on Pseudomonas aeruginosa growth and biofilm formation

Al-Momani

Balawi

Hamed

et al. 2023

Sci Rep

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“…The results revealed that ZnONPs showed very potent activity against the tested microbial biofilms. Also, Abdelraheem and Mohamed [70] ) and the release of Zn 2+ and Cd 2+ [205] ZnO nanostructures (ZnO-NSs) 70-80 S. aureus, S. typhimurium, P. vulgaris, and K. pneumoniae ROS damage to cell membranes [206] confirmed that ZnONPs significantly down-regulated the expression level of all biofilms and virulence genes of P. aeruginosa clinical isolates except the toxA gene.…”

Section: Antimicrobial Activity Of Znonpsmentioning

confidence: 88%

Microbial Mediated Synthesis of Zinc Oxide Nanoparticles, Characterization and Multifaceted Applications

Gomaa

2022

J Inorg Organomet Polym

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Nanoparticles have gained considerable importance compared to bulk counterparts due to their unique properties. Due to their high surface to volume ratio and high reactivity, metallic and metal-oxide nanostructures have shown great potential applications. Among them, zinc oxide nanoparticles (ZnONPs) have gained tremendous attention attributed to their unique properties such as low toxicity, biocompatibility, simplicity, easy fabrication, and environmental friendly. Remarkably, ZnONPs exhibit optical, physical, antimicrobial, anticancer, anti-inflammatory and wound healing properties. These nanoparticles have been applied in various fields such as in biomedicine, biosensors, electronics, food, cosmetic industries, textile, agriculture and environment. The synthesis of ZnONPs can be performed by chemical, physical and biological methods. Although the chemical and physical methods suffer from some disadvantages such as the involvement of high temperature and pressure conditions, high cost and not environmentally friendly, the green synthesis of ZnONPs offers a promising substitute to these conventional methods. On that account, the microbial mediated synthesis of ZnONPs is clean, eco-friendly, nontoxic and biocompatible method. This paper reviews the microbial synthesis of ZnONPs, parameters used for the optimization process and their physicochemical properties. The potential applications of ZnONPs in biomedical, agricultural and environmental fields as well as their toxic aspects on human beings and animals have been reviewed.

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“…Also, ZnO NPs downregulated virulence genes lasA and exoS by 5.2 and 3.7-fold, respectively ( p-value < 0.008). In addition, the toxA gene was downregulated by 1.9-fold (p-value = 0.37) after ZnO NPs remediation, thus no statistically considerable upregulation was recorded 98 .…”

Section: Resultsmentioning

confidence: 95%

Green synthesis of cellulose nanocrystal/ZnO bio-nanocomposites exerting antibacterial activity and downregulating virulence toxigenic genes of food-poisoning bacteria

Dawwam

Al–Shemy

El-Demerdash³

2022

Sci Rep

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Recently, cellulose nanocrystals (CNs) have attracted wide attention owing to their superior properties compared to their bulk materials. For example, they represent an outstanding model for fabricating green metallic/metal oxide nanoparticles (NPs). In this study, two CNs (carboxylated CNs and sulfated CNs) extracted from agro-wastes of palm sheath fibers were used as templates for the facile and green synthesis of ZnO NPs by employing the sono-co-precipitation method. The obtained nanomaterials were characterized using TEM, EDX, UV–visible, DLS, FT-IR, and XRD analysis. As a result, the size and concentration of synthesized ZnO NPs were inversely proportional to one another and were affected by the CNs utilized and the reaction temperature used. Contagious diseases incited by multifarious toxigenic bacteria present severe threats to human health. The fabricated bio-nanocomposites were evaluated in terms of their antimicrobial efficacy by agar well diffusion method and broth microdilution assay, showing that CN–ZnO bio-nanocomposites were effective against the tested Gram-negative (Escherichia coli and Salmonella) and Gram-positive (Listeria monocytogenes and Staphylococcus aureus) bacteria. The influence of the subinhibitory concentrations of these suspensions on the expression of the most critical virulence toxin genes of the tested strains was effective. Significant downregulation levels were observed through toxigenic operons to both fabricated CN–ZnO bio-nanocomposites with a fold change ranging from 0.004 to 0.510, revealing a decline in the capacity and virulence of microorganisms to pose infections. Therefore, these newly fabricated CNS–ZnO bio-nanocomposites could be employed rationally in food systems as a novel preservative to inhibit microbial growth and repress the synthesis of exotoxins.

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The effect of Zinc Oxide nanoparticles on Pseudomonas aeruginosa biofilm formation and virulence genes expression

Cited by 22 publications

References 21 publications

The impact of biosynthesized ZnO nanoparticles from Olea europaea (Common Olive) on Pseudomonas aeruginosa growth and biofilm formation

The impact of biosynthesized ZnO nanoparticles from Olea europaea (Common Olive) on Pseudomonas aeruginosa growth and biofilm formation

Microbial Mediated Synthesis of Zinc Oxide Nanoparticles, Characterization and Multifaceted Applications

Green synthesis of cellulose nanocrystal/ZnO bio-nanocomposites exerting antibacterial activity and downregulating virulence toxigenic genes of food-poisoning bacteria

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