The effectiveness of polyethylene versus titanium particles in inducing osteolysis in vivo

Knoch, Marius von; Jewison, Donna E; Sibonga, Jean D.; Sprecher, Christoph M.; Morrey, B.F.; Löer, F.; Berry, Daniel J.; Scully, Sean P.

doi:10.1016/j.orthres.2003.08.013

Cited by 106 publications

(106 citation statements)

References 25 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…Small animal microCT scanning is widely used for evaluating the outcome of bone remodeling. Our data were consistent with previous studies that UHMWPE particles and other particles types will induce osteolysis [4,45]. We also observed that bone density was also decreased in the contralateral nonoperated femora in the UHMWPE groups compared with the saline group.…”

Section: Discussionsupporting

confidence: 93%

“…By using an advanced fluorescence imaging system, we reduced/erased autofluorescence originating from collagen [27,28], which previously was a major obstacle for fluorescence techniques in bone-related studies. We also detected an increased level of bone remodeling and osteolysis locally in the particle-treated femora similar to the processes of bone remodeling in TJA [1,4,45]. Enhanced bone remodeling stimulated by the infusion of UHMWPE particles is in agreement with the report of Kadoya et al [19] who described similar findings in the bone bed of revised hip arthroplasties.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Continuous Infusion of UHMWPE Particles Induces Increased Bone Macrophages and Osteolysis

Ren

Irani

Huang

et al. 2011

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

Background Aseptic loosening and periprosthetic osteolysis resulting from wear debris are major complications of total joint arthroplasty. Monocyte/macrophages are the key cells related to osteolysis at the bone-implant interface of joint arthroplasties. Whether the monocyte/macrophages found at the implant interface in the presence of polyethylene particles are locally or systemically derived is unknown. Questions/purposes We therefore asked (1) whether macrophages associated with polyethylene particle-induced chronic inflammation are recruited locally or systemically and (2) whether the recruited macrophages are associated with enhanced osteolysis locally.Methods Noninvasive in vivo imaging techniques (bioluminescence and microCT) were used to investigate initial macrophage migration systemically from a remote injection site to polyethylene wear particles continuously infused into the femoral canal. We used histologic and immunohistologic staining to confirm localization of migrated macrophages to the polyethylene particle-treated femoral canals and monitor cellular markers of bone remodeling.Results The values for bioluminescence were increased for animals receiving UHMWPE particles compared with the group in which the carrier saline was infused. At Day 8, the ratio of bioluminescence (operated femur divided by nonoperated contralateral femur of each animal) for the UHMWPE group was 13.95 ± 5.65, whereas the ratio for the saline group was 2.60 ± 1.14. Immunohistologic analysis demonstrated the presence of reporter macrophages in the UHMWPE particle-implanted femora only. MicroCT scans showed the bone mineral density for the group with both UHMWPE particles and macrophage was lower than the control groups. Conclusions Infusion of clinically relevant polyethylene particles, similar to the human scenario, stimulated systemic migration of remotely injected macrophages and local net bone resorption.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Continuous Infusion of UHMWPE Particles Induces Increased Bone Macrophages and Osteolysis

Ren

Irani

Huang

et al. 2011

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

show abstract

“…Compared to our study these discrepancies are due to several differences, including particle concentration ( 1 x lo7 particles), THP-1 differentiation protocol and incubation time (3 h). A recent in vivo study by von Knoch et al [49] supports our notion, because they observed osteolysis, which was induced by detoxified wear particles. In our opinion the effect of LPS-detoxified particles consists particularly in a slight, but significant induction of NF-KB signalling and TNFa-promoter activation leading to a slight release of TNFa.…”

Section: Discussionsupporting

confidence: 83%

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

et al. 2005

View full text Add to dashboard Cite

Wear particles are believed to induce periprosthetic inflammation which contributes to periprosthetic osteolysis. TNFu plays a pivotal role in the pathogenesis of this process. The molecular mechanisms leading to the development of periprosthetic inflammation with upregulated TNFa expression in monocytic cells in response to different wear particles have yet to be defined. 1n:this study we evaluated the effects of polyethylene-and TiAIV-particles on activation of NF-KB signalling pathways and TNFa biosynthesis and release in monocytic cells with respect to periprosthetic osteoclastogenesis. THP-1 monocytic cells were differentiated to macrophage-like cells and exposed to LPS-detoxified polyethylene and prosthesis-derived TiAlV-particles. TNFa release was analyzed in culture supernatant by ELISA. NF-KB activation was examined by electrophoretic mobility shift assay (EMSA), and NF-KB target promoter activities including transactivation of the TNFa promoter were determined by luciferase reporter gene assays. Differentiated THP-1 macrophages were exposed to increasing numbers of particles for 0, 60, 180 and 360 min. Both, polyethylene-and TiAIV-particles induced a significant activation of both NF-KB and TNFa promoters at 180 min. A significant TNFu release was detected after 360 min exposure to polyethylene-and TiAlV-particles in a dose dependent manner. In comparison, LPS induced a much greater activation of NF-KB and TNFa promoters, and TNFa secretion into the supernatant was strongly induced. These results provide evidence that induction of the NF-KB signal transduction pathway in macrophages plays a major role in initiating and mediating the inflammatory response leading to periprosthetic osteolysis.

show abstract

“…Thus, although these particles were larger than the titanium particles (90% C3.6 pm), they were within the critical size range (0.2-10 microns) for phagocytosis-induced macrophage activation by wear particles [44]. Moreover, Ceridust polyethylene particles induce biological effects that are similar to those induced by UHMWPE particles [ 14, 26,40]. Adherent endotoxin was undetectable on the polyethylene particles, which is similar to our previously described "endotoxin-free" titanium particles but at least 500-fold less than titanium particles with adherent endotoxin (Table 1).…”

Section: Characterization Of the Polyethylene Particlesmentioning

confidence: 99%

“…In one model, only a preliminary description has been published [40]. In the second model, osteolysis is studied in bones implanted into air pouches, which therefore lack a blood supply and are more analogous to bone organ culture experiments rather than to the in vivo situation [45,46].…”

Section: Introductionmentioning

confidence: 99%

Polyethylene and titanium particles induce osteolysis by similar, lymphocyte‐independent, mechanisms

Taki¹,

Tatro²,

Nalepka³

et al. 2005

Journal Orthopaedic Research

View full text Add to dashboard Cite

Periprosthetic osteolysis is a major clinical problem that limits the long-term survival of total joint arthroplasties. Osteolysis is induced by implant-derived wear particles, primarily from the polyethylene bearing surfaces. This study examined two hypotheses. First, that similar mechanisms are responsible for osteolysis induced by polyethylene and titanium particles. Second, that lymphocytes do not play a major role in particle-induced osteolysis. To test these hypotheses, we used the murine calvarial model that we have previously used to examine titanium-induced osteolysis. Polyethylene particles rapidly induced osteolysis in the murine calvaria 5-7days after implantation. The polyethylene-induced osteolysis was associated with large numbers of osteoclasts as well as the formation of a thick periosteal fibrous tissue layer with numerous macrophages containing phagocytosed polyethylene particles. Polyethylene-induced osteolysis was rapidly repaired and was undetectable by day 21 after implantation. Lymphocytes were noted in the fibrous layer of wild-type mice. However, the amount of osteolysis and cytokine production induced by polyethylene particles was not substantially affected by the lack of lymphocytes in PfplRag2 double knock out mice. All of these findings are similar to our observations of osteolysis induced by titanium particles. These results provide strong support for both of our hypotheses: that similar mechanisms are responsible for osteolysis induced by polyethylene and titanium particles and that lymphocytes do not play a major role in particle-induced osteolysis.

show abstract

The effectiveness of polyethylene versus titanium particles in inducing osteolysis in vivo

Cited by 106 publications

References 25 publications

Continuous Infusion of UHMWPE Particles Induces Increased Bone Macrophages and Osteolysis

Continuous Infusion of UHMWPE Particles Induces Increased Bone Macrophages and Osteolysis

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

Polyethylene and titanium particles induce osteolysis by similar, lymphocyte‐independent, mechanisms

Contact Info

Product

Resources

About