The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees

Quainoo, A. K.; Wetten, A.; Allainguillaume, Joël

doi:10.1016/j.jviromet.2008.01.007

Cited by 28 publications

(20 citation statements)

References 15 publications

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“…The primary embryos infected by GFLV may have a limited attitude to secondary embryogenesis and/or young secondary embryos regenerated from the primary GFLV-infected embryos may escape virus invasion. A similar situation was indeed observed by Quainoo et al (2008) in somatic embryos of cacao: the Cocoa swollen shoot virus was gradually excluded from primary to secondary embryos.…”

Section: Discussionsupporting

confidence: 77%

“…Meristem tip culture is particularly effective in eliminating phloem-limited viruses, while thermotherapy is normally required for the elimination of viruses that readily invade plant meristems, such as nepovirus. Somatic embryogenesis, usually adopted to regenerate plantlets in biotechnological breeding programs, successfully eradicated viruses from Citrus (D' Onghia et al 2001), sugarcane (Parmessur et al 2002) and cacao (Quainoo et al 2008). In grapevine it efficiently eliminated several phloem-limited viruses (Goussard et al 1991;) and recently two nepovirus, GFLV (Gambino et al 2009) and Arabis mosaic virus (Borroto-Fernandez et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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In situ localization of Grapevine fanleaf virus and phloem-restricted viruses in embryogenic callus of Vitis vinifera

Gambino¹,

Vallania²,

Gribaudo³

2010

Eur J Plant Pathol

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In grapevine, somatic embryogenesis is particularly effective in eliminating several important virus diseases. However, the mechanism whereby regenerated somatic embryos are freed of the viruses is not clear. The distribution of Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus-3 (GLRaV-3) and Grapevine virus A (GVA) in embryogenic callus of grapevine was investigated by in situ hybridization using digoxygenin-labelled oligonucleotide probes. Four months after culture initiation, in callus originated by GFLV-infected explants we observed a mosaic of infected and uninfected cells, with high concentrations of viruses in some cell groups in peripheral zones of the callus. In addition some abnormal somatic embryos showed a high hybridization signal. In callus originated by GVAand GLRaV-3-infected explants the viruses were concentrated in few cells surrounded by areas of virus-free cells. The two viruses were generally localized in different clusters of cells inside the callus and the levels of infection were lower than those observed in GFLV-infected callus. No virus was detected in callus nor in somatic embryos after 6 months of culture. The results highlight the difficulties of some viruses at stably invading callus tissues and the differential ability of GFLV to spread in the callus cells compared to the phloem-limited viruses.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

In situ localization of Grapevine fanleaf virus and phloem-restricted viruses in embryogenic callus of Vitis vinifera

Gambino¹,

Vallania²,

Gribaudo³

2010

Eur J Plant Pathol

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“…Thermotherapy, even in association with other techniques such as tissue culture, represents the most fre- Goussard & Wiid, 1992;Popescu et al, 2003Popescu et al, , 2010Gambino et al, 2006Gambino et al, , 2010Gribaudo et al, 2006;Borroto-Fernández et al, 2009;Youssef et al, 2009Ramos & Zamora, 1999D'Onghia et al, 2001;Golino et al, 2007;Quainoo et al, 2008Fitch et al, 2001Parmessur et al, 2002;Ramgareeb et al, 2010Ebi et al, 2000Ayabe & Sumi, 2001;Ramírez-Malagón et al, 2006Truskinov & Rogozina, 1997Mahmoud et al, 2009Wangai & Bock, 1996Morris et al, 1997;S edivá et al, 2006;Kumar et al, 2009;Kabir et al, 2010 quently used technique for sanitation over the last 20 years, while applications of methods such as chemotherapy and tissue culture represent the main topic in plant sanitation research.…”

Section: Comparison Of Techniquesmentioning

confidence: 99%

Review. Elimination of viruses in plants: twenty years of progress

Panattoni

Luvisi

Triolo

2013

Span. j. agric. res.

145

112

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To shed light on trends about elimination of viruses from plants, a bibliographic research was conducted to identify thermotherapy, chemotherapy and tissue culture trials published from 1991 through 2010. Among woody plants, grapevine, apple and peach are the most frequent targets of sanitation protocols because their health status is strictly regulated. Even if thermotherapy represents the preferred method for the host, grapevine viruses can also be eliminated with chemotherapy and tissue culture; apple viruses respond to chemotherapy as well. Although a similar trend was reported among herbaceous plants, chemotherapy was the most frequently used technique in potato. With regard to virus, thermotherapy was successfully applied against viruses belonging to 13 families and an unassigned genus. Instead, chemotherapy and tissue culture techniques eradicated viruses belonging to fewer families (nine). An interpretation of thermotherapy effects considers the new metabolic “pathways” triggered by the natural antiviral response emitted by the infected plant, with particular reference to virus-induced gene silencing. With regard to chemotherapy, several groups of antiviral drugs belong to inosine monophosphate dehydrogenase inhibitors, S-adenosylhomocysteine hydrolase inhibitors, neuraminidase inhibitors. Tissue culture, usually adopted to regenerate plantlets in biotechnological breeding programs, represents the less used tool for eliminate viruses from plants.

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“…In cacao, in vitro embryogenesis or somatic embryogenesis (SE) is an alternative to traditional propagation methods and allows rapid clonal propagation of true‐to‐type plants with normal dimorphic architecture and taproot formation. Importantly, this system has shown to be an effective method in propagation of CSSV disease‐free plantlets (Quainoo et al ., ). Moreover, a study conducted by López and co‐workers in 2010 found that de novo genetic mutations and epigenetic modifications do not accumulate with ageing of in vitro induced cacao calli (López et al ., ); this conclusion is also relevant to a recent study of long‐term SE (Quinga et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Cacao biotechnology: current status and future prospects

Wickramasuriya

Dunwell

2017

Plant Biotechnology Journal

108

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Summary Theobroma cacao—The Food of the Gods, provides the raw material for the multibillion dollar chocolate industry and is also the main source of income for about 6 million smallholders around the world. Additionally, cocoa beans have a number of other nonfood uses in the pharmaceutical and cosmetic industries. Specifically, the potential health benefits of cocoa have received increasing attention as it is rich in polyphenols, particularly flavonoids. At present, the demand for cocoa and cocoa‐based products in Asia is growing particularly rapidly and chocolate manufacturers are increasing investment in this region. However, in many Asian countries, cocoa production is hampered due to many reasons including technological, political and socio‐economic issues. This review provides an overview of the present status of global cocoa production and recent advances in biotechnological applications for cacao improvement, with special emphasis on genetics/genomics, in vitro embryogenesis and genetic transformation. In addition, in order to obtain an insight into the latest innovations in the commercial sector, a survey was conducted on granted patents relating to T. cacao biotechnology.

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The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees

Cited by 28 publications

References 15 publications

In situ localization of Grapevine fanleaf virus and phloem-restricted viruses in embryogenic callus of Vitis vinifera

In situ localization of Grapevine fanleaf virus and phloem-restricted viruses in embryogenic callus of Vitis vinifera

Review. Elimination of viruses in plants: twenty years of progress

Cacao biotechnology: current status and future prospects

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