PurposeThis study investigates the impact of staff training on university performance (UP), focusing particularly on the mediating role of ISO 9001 implementation in the higher education (HE) sector.Design/methodology/approachEmploying a quantitative research design, data were collected via online surveys using purposive sampling techniques from academic and administrative staff affiliated with ISO 9001-certified universities. Subsequent analysis utilized SmartPLS-4 software.FindingsThe findings indicate that staff training significantly influences both ISO 9001 standard implementation and UP. Additionally, the mediating effect of ISO 9001 in the relationship between staff training and UP was found to be positive and significant.Research limitations/implicationsLimitations of the study include its cross-sectional design, reliance on purposive sampling and exclusive focus on academic and administrative staff from seven ISO-certified universities in Pakistan, potentially limiting generalizability. Nonetheless, the study enriches the discourse on quality management in HE by emphasizing the role of staff training in fostering knowledge creation and enhancing staff competencies within organizational learning theory (OLT), and by integrating ISO 9001 into the dynamic capability theory (DCT) framework.Practical implicationsThe study provides practical insights for policymakers, administrators and quality managers, emphasizing the importance of staff training, resource allocation for compliance and continuous improvement efforts to effectively implement ISO 9001 requirements and enhance overall UP.Originality/valueThis study contributes by introducing ISO 9001 adoption as a mediator between staff training and UP while integrating OLT and DCT theories within the university context.