The Effects of a Phenothiazine Derivative (RP.3300) on Red Cell Preservation

The Effects of Phenothiazines on Human Erythrocytes: An Ultrastructural Study

“…The in vitro effects of various phenothiazine derivatives on normal erythrocytes have been extensively studied (Chaplin et al, 1952; Ahtee, 1966; van Steveninck et al, 1967). The antihaemolytic effect of the phenothiazines prompted Laforet & Gardner (1968) to examine the possibility of using these drugs in the treatment of paroxysmal nocturnal haemoglobinuria (PNH).…”

“…A number of workers have studied the effects of phenothiazine derivatives on red blood cells (Chaplin et al, 1952; Ahtee, 1966; van Steveninck et al, 1967). The majority of these have emphasized the reduction which occurs in osmotic fragility following incubation of erythrocytes with these drugs (Chaplin et al, 1952;Freeman & Spirtes, 1963). Although the exact nature of this apparently protective mechanism is not understood it is possibly mediated through the effects of the drugs on the cell surface membrane.…”

Antihemolytic and Antiproteolytic Properties of Substituted Thiosemicarbazidophenothiazines and Thiazolidonylphenothiazines

“…The in vitro inhibition of trypsin-induced hydrolysis of bovine serum albumin by these phenothiazines was concentration dependent and competitive in nature; the degree of inhibition ranged from 30 to 50 and 32 to 79% for substituted thiosemicarba-It has been shown that the membrane-stabilizing effects of phenothiazine derivatives are reflected by delayed spontaneous hemolysis of red blood cells (1,2). , and VIRGIL I. STENBERG * Abstract Antihemolytic and antiproteolytic properties of several 10-(1-acetyl-4-arylthiosemicarbazido)phenothiazines and their corresponding cyclized l0-(2-arylimino-3-acetylamino-4-thiazoli-dony1)phenothiazines were investigated.…”

“…Keyphrases Diphenhydramine hydrochloride-spectrophotometric analysis, color complex with dipicrylamine, pharmaceutical formulations Dipicrylamine-color complex formation for spectrophotometric analysis of diphenhydramine hydrochloride, pharmaceutical formulations Spectrophotometry-analysis, diphenhydramine hydrochloride, pharmaceutical formulations Antihistaminic agents-diphenhydramine hydrochloride, spectrophotometric analysis, pharmaceutical formulations 5-(Hydroxymethyl)-2-furaldehyde is a well-known product of the Maillard reaction (1)(2)(3). It is frequently present in pharmaceutical preparations, particularly in syrups, and can interfere in the chemical analysis for active components.…”

Effect of Additions to ACD Blood on Erythrocyte Preservation.

“…Chaplin et aZ. (10) investigated the effect of a related phenothiazine derivative (R.P. 3300) on red cell preservation, and found that their compound interfered with the hemolysis of fresh red cells by hypotonic NaC1 solution.…”