The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells

Jeong, Youngdan; Yang, Wenjun; Ko, Hyunjung; Kim, Miri

doi:10.5395/rde.2014.39.3.187

Cited by 18 publications

(15 citation statements)

References 35 publications

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“…Among them, BMP-2 is the most effective inducer of proliferation, differentiation, and mineralization in osteoblasts (Higuchi et al, 2002, Hiraki et al, 1991). Previous studies have shown that MTA promoted osteoblastic mineralization concomitant with increased BMP-2 mRNA expression (Ghasemi et al, 2014, Jeong et al, 2014). Here, we also investigated MTA-induced osteoblastic mineralization without BMP-2 mRNA elevation compared to controls.…”

Section: Discussionmentioning

confidence: 91%

Mineral trioxide aggregate induces osteoblastogenesis via Atf6

et al. 2015

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Mineral trioxide aggregate (MTA) has been recommended for various uses in endodontics. To understand the effects of MTA on alveolar bone, we examined whether MTA induces osteoblastic differentiation using MC3T3-E1 cells. MTA enhanced mineralization concomitant with alkaline phosphatase activity in a dose- and time-dependent manner. MTA increased production of collagens (Type I and Type III) and matrix metalloproteinases (MMP-9 and MMP-13), suggesting that MTA affects bone matrix remodeling. MTA also induced Bglap (osteocalcin) but not Bmp2 (bone morphogenetic protein-2) mRNA expression. We observed induction of Atf6 (activating transcription factor 6, an endoplasmic reticulum (ER) stress response transcription factor) mRNA expression and activation of Atf6 by MTA treatment. Forced expression of p50Atf6 (active form of Atf6) markedly enhanced Bglap mRNA expression. Chromatin immunoprecipitation assay was performed to investigate the increase in p50Atf6 binding to the Bglap promoter region by MTA treatment. Furthermore, knockdown of Atf6 gene expression by introduction of Tet-on Atf6 shRNA expression vector abrogated MTA-induced mineralization. These results suggest that MTA induces in vitro osteoblastogenesis through the Atf6–osteocalcin axis as ER stress signaling. Therefore, MTA in endodontic treatment may affect alveolar bone healing in the resorbed region caused by pulpal infection.

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Section: Discussionmentioning

confidence: 91%

Mineral trioxide aggregate induces osteoblastogenesis via Atf6

et al. 2015

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“…However, the varying ratio of the growth factors mobilized by MTA affects the nature of the tissue repair. In this regard, there have been several attempts at combining MTA with factors such as fibroblast growth factor‐2, bone morphogenetic protein‐2 (BMP‐2) and enamel matrix derivative, respectively . While these have been successful in enhancing the proliferation and differentiation of pulpal cells, at times, an unfavorable relationship between the released growth factors and the extracellular matrix exists which results in apoptosis (instead of repair).…”

Section: Endodontic Materialsmentioning

confidence: 99%

“…In this regard, there have been several attempts at combining MTA with factors such as fibroblast growth factor-2, bone morphogenetic protein-2 (BMP-2) and enamel matrix derivative, respectively. [48][49][50] While these have been successful in enhancing the proliferation and differentiation of pulpal cells, at times, an unfavorable relationship between the released growth factors and the extracellular matrix exists which results in apoptosis (instead of repair). In addition, these cell death pathways are also activated at high concentrations of MTA (200 mg/mL).…”

Section: 2637mentioning

confidence: 99%

Oroactive dental biomaterials and their use in endodontic therapy

et al. 2019

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Dental biomaterials have revolutionized modern therapies. Untreated dental caries remains the major etiological factor for endodontic treatment, and together with a decreasing rate of tooth loss escalates the importance of continuously improving the materials used for endodontic therapies. Endodontic biomaterials are used for vital pulp therapies, irrigation, intracanal medicaments, obturation and regenerative procedures. These materials offer several functions including: antimicrobial activity, mechanical reinforcement, aesthetics, and therapeutic effects. Vital pulp therapies have seen an improvement in clinical results with an incremental approach to build on the strengths of past materials such as calcium hydroxide and calcium silicates. While sodium hypochlorite remains the gold standard for canal irrigation, numerous nanoparticle formulations have been developed to promote sustained antimicrobial action. Gutta‐percha based bulk fillers remain the most common materials for root filling. However, while multiple studies focus on the development of novel formulations containing drugs, glass derivatives or ionic‐, polymeric‐, or drug‐ loaded nanoparticles, a lack of reliable and long‐term clinical evidence obligates further study as experienced clinicians prefer to use what has worked for decades. This review delves in to the biochemistry of the materials to scrutinize their shortcomings, and where opportunity lies to further enhance their efficacy in endodontic practice. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:201–212, 2020.

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“…One microgram RNA was reverse-transcribed with oligo (dT) 15 primers by using AccuPower RT PreMix (iNtRON Biotechnology, Gyeonggi-do, South Korea). Next, the generated cDNAs were amplified with AccuPower PCR PreMix (Bioneer Corporation, Daejeon, South Korea).…”

Section: Rna Isolation and Reverse Transcriptase Polymerase Chain Reamentioning

confidence: 99%

“…Moreover, we demonstrated that a combination of MTA and enamel matrix derivative promoted more rapid differentiation in HDPCs, as compared with pure MTA (14). In addition, the MTA/bone morphogenetic protein-2 (BMP-2) combination promoted more rapid differentiation in MC3T3-E1 cells than did MTA or enamel matrix derivative alone (15). MTA mixed with hydration accelerators such as calcium chloride and low-dose citric acid more upregulated osteogenic genes than that of MTA alone in mouse osteoblasts (5).…”

mentioning

confidence: 94%