The effects of cold, light and time of day during low-temperature shift on the expression of CBF6, FpCor14b and LOS2 in Festuca pratensis

Jurczyk, Barbara; Rapacz, Marcin; Budzisz, Katarzyna; Barcik, Weronika; Sasal, Monika

doi:10.1016/j.plantsci.2011.08.004

Cited by 24 publications

(12 citation statements)

References 28 publications

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“…It is interesting to find down-regulated genes in the AP2/ERF family, as these suggest that the interaction of light and temperature is of special importance for plants during the CA process. Catalá et al [50] and Jurczyk et al [51] have also reported that light is required for full CA in Arabidopsis and Festuca pratensis .…”

Section: Resultsmentioning

confidence: 99%

Global transcriptome profiles of Camellia sinensis during cold acclimation

et al. 2013

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BackgroundTea is the most popular non-alcoholic health beverage in the world. The tea plant (Camellia sinensis (L.) O. Kuntze) needs to undergo a cold acclimation process to enhance its freezing tolerance in winter. Changes that occur at the molecular level in response to low temperatures are poorly understood in tea plants. To elucidate the molecular mechanisms of cold acclimation, we employed RNA-Seq and digital gene expression (DGE) technologies to the study of genome-wide expression profiles during cold acclimation in tea plants.ResultsUsing the Illumina sequencing platform, we obtained approximately 57.35 million RNA-Seq reads. These reads were assembled into 216,831 transcripts, with an average length of 356 bp and an N50 of 529 bp. In total, 1,770 differentially expressed transcripts were identified, of which 1,168 were up-regulated and 602 down-regulated. These include a group of cold sensor or signal transduction genes, cold-responsive transcription factor genes, plasma membrane stabilization related genes, osmosensing-responsive genes, and detoxification enzyme genes. DGE and quantitative RT-PCR analysis further confirmed the results from RNA-Seq analysis. Pathway analysis indicated that the “carbohydrate metabolism pathway” and the “calcium signaling pathway” might play a vital role in tea plants’ responses to cold stress.ConclusionsOur study presents a global survey of transcriptome profiles of tea plants in response to low, non-freezing temperatures and yields insights into the molecular mechanisms of tea plants during the cold acclimation process. It could also serve as a valuable resource for relevant research on cold-tolerance and help to explore the cold-related genes in improving the understanding of low-temperature tolerance and plant-environment interactions.

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Section: Resultsmentioning

confidence: 99%

Global transcriptome profiles of Camellia sinensis during cold acclimation

et al. 2013

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“…The concentration and quality of the RNA and cDNA were assessed using a Q5000 UV-Vis spectrophotometer (Quawell, San Jose, CA, United States). The PCR amplifications of the HSP90, HSP70, HSP18, and HSP17 transcripts were run in triplicate as described by Jurczyk et al [52]. The primers and probes were designed using Primer Express Software v 3.0.1 (Applied Biosystems by Life Technologies, Foster City, CA, USA).…”

Section: Isolation Of the Membrane And Cytosolic Fractionsmentioning

confidence: 99%

HSP Transcript and Protein Accumulation in Brassinosteroid Barley Mutants Acclimated to Low and High Temperatures

Sadura

Libik-Konieczny

Jurczyk

et al. 2020

IJMS

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In temperature stress, the main role of heat-shock proteins (HSP) is to act as molecular chaperones for other cellular proteins. However, knowledge about the hormonal regulation of the production of the HSP is quite limited. Specifically, little is known about the role of the plant steroid hormones—brassinosteroids (BR)—in regulating the HSP expression. The aim of our study was to answer the question of how a BR deficit or disturbances in its signaling affect the accumulation of the HSP90, HSP70, HSP18, and HSP17 transcripts and protein in barley growing at 20 °C (control) and during the acclimation of plants at 5 °C and 27 °C. In barley, the temperature of plant growth modified the expression of HSPs. Furthermore, the BR-deficient mutants (mutations in the HvDWARF or HvCPD genes) and BR-signaling mutants (mutation in the HvBRI1 gene) were characterized by altered levels of the transcripts and proteins of the HSP group compared to the wild type. The BR-signaling mutant was characterized by a decreased level of the HSP transcripts and heat-shock proteins. In the BR-deficient mutants, there were temperature-dependent cases when the decreased accumulation of the HSP70 and HSP90 transcripts was connected to an increased accumulation of these HSP. The significance of changes in the accumulation of HSPs during acclimation at 27 °C and 5 °C is discussed in the context of the altered tolerance to more extreme temperatures of the studied mutants (i.e., heat stress and frost, respectively).

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“…Short photoperiods and low R:FR ratios can interact with temperature to induce freezing resistance in A. thaliana, and this effect is mediated by the circadian clock through its control of CBF expression [42,43]. In wheat and barley, low R:FR ratios upregulate expression of CBF14 as well as freezing resistance [44], and in meadow fescue CBF6 expression is affected by interactions between temperature, light quality, and irradiance [94]. In A. thaliana, CBFs can down-regulate leaf growth by down-regulating the content of gibberellic acid (GA), thereby allowing the accumulation of DELLA proteins which inhibits growth, and in addition increases freezing resistance by a reduction of ROS [95,96].…”

Section: Regulation Of Leaf Growth Versus Cold Acclimationmentioning

confidence: 99%

Optimal Regulation of the Balance between Productivity and Overwintering of Perennial Grasses in a Warmer Climate

Ergon

2017

Agronomy

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Seasonal growth patterns of perennial plants are linked to patterns of acclimation and de-acclimation to seasonal stresses. The timing of cold acclimation (development of freezing resistance) and leaf growth cessation in autumn, and the timing of de-acclimation and leaf regrowth in spring, is regulated by seasonal cues in the environment, mainly temperature and light factors. Warming will lead to new combinations of these cues in autumn and spring. Extended thermal growing seasons offer a possibility for obtaining increased yields of perennial grasses at high latitudes. Increased productivity in the autumn may not be possible in all high latitude regions due to the need for light during cold acclimation and the need for accumulating a carbohydrate storage prior to winter. There is more potential for increased yields in spring due to the availability of light, but higher probability of freezing events in earlier springs would necessitate a delay of de-acclimation, or an ability to rapidly re-acclimate. In order to optimize the balance between productivity and overwintering in the future, the regulation of growth and acclimation processes may have to be modified. Here, the current knowledge on the coordinated regulation of growth and freezing resistance in perennial grasses is reviewed.

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The effects of cold, light and time of day during low-temperature shift on the expression of CBF6, FpCor14b and LOS2 in Festuca pratensis

Cited by 24 publications

References 28 publications

Global transcriptome profiles of Camellia sinensis during cold acclimation

Global transcriptome profiles of Camellia sinensis during cold acclimation

HSP Transcript and Protein Accumulation in Brassinosteroid Barley Mutants Acclimated to Low and High Temperatures

Optimal Regulation of the Balance between Productivity and Overwintering of Perennial Grasses in a Warmer Climate

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