The effects of salinity on the Manila clam (Ruditapes philippinarum) using the neutral red retention assay with adapted physiological saline solutions

Coughlan, B.M.; Moroney, G.A.; Pelt, Frank N.A.M. van; O’Brien, Nora M.; Davenport, John; O’Halloran, John

doi:10.1016/j.marpolbul.2009.06.020

Cited by 55 publications

(22 citation statements)

References 44 publications

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“…Several authors have reported baseline haemocyte DNA damage in M. edulis (Large et al 2002;Mamaca et al 2005) and M. galloprovincialis (Nigro et al 2006) that are much higher than those found in the present study and in previous work published by the authors (Coughlan et al, 2002;Hartl et al, 2004;Hartl et al, 2010). Although not explicitly indicated, this may be due to the application of uncorrected tissue culture media, an issue that has also plagued other ecotoxicity biomarker tests (Coughlan et al 2009). …”

Section: Discussioncontrasting

confidence: 69%

Fluctuating estuarine conditions are not confounding factors for the Comet assay assessment of DNA damage in the mussel Mytilus edulis

Singh¹,

Hartl²

2012

Ecotoxicology

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The Comet assay is finding increasing application as a biomarker assay for the genotoxic potential of contaminants in field transplantation experiments involving mussels. Especially in estuaries, habitats that are of particular concern, environmental variables, such as salinity, can vary significantly. Although hinted at in the literature, there is a lack of clarification as to whether changes in salinity or emersion-induced hypoxia have the potential to alter background DNA damage in mussels, thus masking the extent of potential genotoxic effects following exposure to environmental contaminants. The present study exposed Mytilus edulis in the laboratory to static salinities (25, 50, 75, and 100 %) for 72 h. Mussels were also subjected to simulated tidal cycles, including periods of emersion, for 72 h. None of these treatments resulted in a significant change in the level of DNA damage expressed as % tail DNA. These experiments demonstrate that salinity, within the limits of the concentrations tested, and temporary emersion are not confounding factors for Comet assay data derived from M. edulis.

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Section: Discussioncontrasting

confidence: 69%

Fluctuating estuarine conditions are not confounding factors for the Comet assay assessment of DNA damage in the mussel Mytilus edulis

Singh¹,

Hartl²

2012

Ecotoxicology

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“…Many studies used transplanted clams to assess sediment quality, but NRR assay was performed using other species, e.g. R. philippinarum (Nigro et al 2006;Coughlan et al 2009) or Anomalocardia brasiliana (Martins et al 2005). On the contrary, NRR assay was previously used in field trials with C. maenas in order to demonstrate differences between polluted and non-polluted sites (Wedderburn et al 1998;Brown et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless improvements and modifications are needed in order to obtain a still more functional and accurate screening tool and to increase its capability as a low-cost, quick, reliable and sensitive biomarker for future studies (Coughlan et al 2009). The methodology could be improved by reducing interval times between slides observation at the microscope from 15 to 10 min, in order better appreciate differences from stressed and healthy organisms.…”

Section: Discussionmentioning

confidence: 99%

Application of neutral red retention assay to caged clams (Ruditapes decussatus) and crabs (Carcinus maenas) in the assessment of dredged material

et al. 2011

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Dredged material management is a key issue for the protection of aquatic environments. The in situ approach using caged bioindicator species has been chosen lately as a new methodology for the assessment of dredged material. In a tier testing approach, neutral red retention (NRR) assay has been applied as a screening tool to detect adverse changes in health status associated with contamination. Nevertheless, to authors' knowledge, little is known about the application and validation of this technique in sediment bioindicator species and under field conditions. Caged Ruditapes decussatus and Carcinus maenas were exposed during 28 days to potentially contaminated sediments at three sites in Algeciras Bay (SW Spain) and one site in Cádiz Bay (SW Spain). Lysosomal membrane stability was measured over time in haemolymph samples of exposed clams and crabs using the NRR assay. Sediment characterization of the study sites was performed in parallel. NRR time did not vary significantly (p > 0.05) over time in organisms from Cádiz Bay. Conversely, significant differences (p < 0.05) in NRR time were found in clams and crabs exposed to sediments from Algeciras Bay, which exhibited a 30-70% decrease in haemocyte lysosome membrane stability compared to day 0. Statistical analysis showed a strong correlation between the drop of haemocyte lysosome membrane stability, in both crabs and clams, and the presence of metals (p < 0.05) and PAHs (p < 0.01) in the studied sediments. The results obtained confirmed the use of NRR assay as a suitable and sensitive method to be used in the assessment of sediment quality using as bioindicator species the clam R. philippinarum and the crab C. maenas.

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“…의 생식소발달과 연령 및 성장 연구 (Chung et al, 1994), 진해 연안산 바지락의 자원생태학적 연구 (Kim and Zhang, 1999), 용존산소의 변화에 따른 바지락의 생리적 반응 연구 (Shin et al, 2001), 한산도 진두해역 양식 바지락의 자원생 태학적 연구 (Cho and Jeong, 2007), 통영 연기해역의 바지 락자원 적정관리에 관한 연구 (Cho et al, 2008) 등이 있고, 국외에서는 러시아 Amursky 만의 바지락의 개체군 구조와 성장연구 (Ponurovskii, 2008), 중국 Jiaozhou만에 이식한 바지락 성장과 관련한 연구 (Ren et al, 2008), 바지락의 서 식환경에 대한 실험연구 (Coughlan et al, 2009) …”

Section: 바지락에 관한 국내 연구로는 김제연안에 서식하는 바지락unclassified

The study of stock assessment and management implications of the Manila clam, Ruditapes philippinarum in Taehwa river of Ulsan

Choi¹,

Yoon²,

Lee³

et al. 2011

The Korean Journal of Malacology

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The manila clam (Ruditapes philippinarum) is mainly distributed in the coastal area which consist of mud, sand and gravel, but they rarely live on the upper and down reaches of river. For a long time the manila clam has been inhabited in Taehwa river which has been exploited as a traditional earning resources and has become as a major object by neighborhood fishermen. This study was undertaken to evaluate stock assessment and to build management implications with the ecological parameters in Taehwa river from June 2009 to June 2010. The maximum age of manila clam was determined to 6 years old from observing ring radius of shell, the length and weight relationship was TW = 0.0002SL 3.063 (R 2 = 0.925). K and L∞ were respectively estimated 46.64 mm and 0.341/year by von Bertalanffy growth. The instantaneous total mortality was estimated to be 1.171/year and the age at first capture was 1.37 years by the Pauly's method using shell length composition. The current total biomass of manila clam was calculated 1,483 mt over study area 1.46 km 2 by swept area method. ABC (Acceptable Biological Catch) estimates of manila clam showed 512 mt with using F0.1. It's desirable to determine the optimum harvesting time as after main spawning season, as well as it's required to manage fisheries resources considering capture age and biomass through adjusting a first age at capture.

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The effects of salinity on the Manila clam (Ruditapes philippinarum) using the neutral red retention assay with adapted physiological saline solutions

Cited by 55 publications

References 44 publications

Fluctuating estuarine conditions are not confounding factors for the Comet assay assessment of DNA damage in the mussel Mytilus edulis

Fluctuating estuarine conditions are not confounding factors for the Comet assay assessment of DNA damage in the mussel Mytilus edulis

Application of neutral red retention assay to caged clams (Ruditapes decussatus) and crabs (Carcinus maenas) in the assessment of dredged material

The study of stock assessment and management implications of the Manila clam, Ruditapes philippinarum in Taehwa river of Ulsan

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