The Effects of SNEDDS (Self-Nano Emulsifying Drug Delivery System) Black Cumin Oil Seeds (Nigella sativa L) to The Histopatology of Lung Organs of The Sprague Dawley Rats That Induced by DMBA

Abstract

Lung cancer is the cancer that caused death, especially for men in Indonesia. One of the compounds that causes cancer is 712-Dimethylben(a)anthracene (DMBA). Black cumin seed oils (BCSO) is able to reduce the damage of lung cells to the DMBA-induced rat. To improve total medicine that could arrive and react at the cancer cell, it's necessary to make selfnanoemulsifying drug delivery system (SNEDDS). The aim of this research is to know the effect of SNEDDS BCSO into of histopathological lung organ of the Sprague Dawley (SD) rat that's already DMBA-induced. The making of SNEDDS is by using BCSO, tween 80 and PEG 400. Treatment was done in vivo towards 25 SD male rats, which are divided to 4 groups. The first group act as the baseline, the second one are given with the DMBA, the third group was given BCSO as equivalent as thymoquinone 6.8 mg/KgBW, and group 4 was given BCSO SNEDDS as equivalent as 6.8 mg/KgBW of thymoquinone. Next, the rat discharged and lung organs are taken then being H & E painted. Based on results of histopathology lung in a descriptive manner, there's no specific differences of histopathology between the group treatment. Next, scoring is done based on alveolar category damage. After that, was done the Kruskal Wallis test, which obtained P value = 0.801. Because of the p value was bigger than 0.05, then this statistical test didn't show any significant difference at each of the group. This research concludes that there is no influence from BCSO SNEDDS to histopathology of DMBA-induced lung compared with BCSO administration only.

Formulation And Antioxidant Property Of Bitter Melon Seeds Oil Loaded Into SNEDDS Systems As A Nutraceutical

“…The % inhibition of non-SNEDDS black cumin seeds extract was about 2.6% and after being formulated into SNEDDS preparation increased to 73%. Wahyuningsih and Khoirunnisa (2019) showed no influence of SNEDDS formulation on the histopathology of DMBA-induced lung organs compared with Black cumin seed oil administration only. These different results might be influenced by the physical properties of the nanoemulsion, the stability of the molecule, and the partition of the antioxidant compounds in the lipid phase (Jusnita and Syurya, 2019).…”

Formulasi dan Karakterisasi SNEDDS (Self Nanoemulsifying Drug Delivery System) Mengandung Minyak Jintan Hitam dan Minyak Zaitun

“…Penelitian lainnya juga menunjukkan bahwa formulasi SNEDDS mampu meningkatan disolusi dan biovailabilitas oral dari beberapa senyawa aktif [11]. Penelitian lainnya berhasil memformulasi sediaan SNEDDS yang mengandung minyak jintan hitam tunggal [12]. Pada penelitian ini, dikembangkan sediaan SNEDDS mengandung kombinasi minyak jintan hitam dan minyak zaitun yang diketahui memiliki efek sinergis sebagai antihiperlipidemia.…”

PENGARUH PEMBERIAN EKSTRAK BAWANG PUTIH (Allium sativum) TERHADAP GAMBARAN HISTOPATOLOGI PARU-PARU TIKUS Sprague dawley YANG DIINDUKSI DENGAN 7,12 Dimetilbenz(a)antrasen (DMBA)

“…(4) Pengobatan kanker dengan kemoterapi masih memiliki kelemahan karena selain sel kanker juga mempengaruhi sel-sel normal dan menghasilkan efek samping fisik dan psikis Antara lain seperti kelelahan, mual, muntah, rambut rontok, kecemasan, rasa malu, ketidakberdayaan, depresi, bahkan pada kasus yang parah dapat menyebabkan kematian. (6) Dengan beragamnya efek samping yang timbul akibat pengobatan secara medis tersebut, maka dibutuhkan penemuan pengobatan baru yang selektif membunuh sel kanker tanpa mempengaruhi sel normal. Di masyarakat sendiri sudah banyak beredar isu tentang manfaat bawang putih sebagai antikanker tanpa landasan bukti ilmiah.…”