The effects of tea polyphenol‐ozonated slurry ice treatment on the quality of large yellow croaker (Pseudosciaena crocea) during chilled storage

Abstract

BACKGROUND:The aim of the current study was to evaluate the synergistic effects of tea polyphenol-ozonated slurry ice on the quality, physicochemical and protein characteristics of large yellow croaker (Pseudosciaena crocea) during chilled (4 °C) storage. To 0.3% tea polyphenol combined with ozone water was added sodium chloride until the salt concentration reached 3.3% and with the use of an ice machine the mixture formed the tea polyphenol-ozonated slurry ice. Microbial [total viable count (TVC)], physicochemical [total volatile basic nitrogen (TVB-N), K value], myofibrillar fragmentation index (MFI), Ca 2+ -ATPase activity, total sulfhydryl content, intrinsic fluorescence intensity (IFI), Fourier-transform infrared (FTIR), scanning electron microscopy (SEM) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were analyzed during chilled (4 °C) storage for up to 20 days. RESULTS:The results showed that tea polyphenol-ozonated slurry ice could effectively inhibit the increase of TVC and TVB-N, reduce the degree of adenosine triphosphate (ATP) degradation. In addition, the tea polyphenol-ozonated slurry ice treatment could protect the integrity of myosin in myofibrillar proteins (MPs) by inhibiting the decrease of Ca 2+ -ATPase activity and the content of total sulfhydryl. Furthermore, the tea polyphenol-ozonated slurry ice presented a superiorly protective effect on protein structure in MPs as manifested by the results of IFI, FTIR and SDS-PAGE. It was possible that due to the addition of tea polyphenol, the antioxidant activity of this complex was significantly improved.CONCLUSION: The tea polyphenol-ozonated slurry ice treatment can maintain the quality of large yellow croaker by decreasing the damage of MP caused by the interaction between microorganisms and endogenous enzymes.

Enhancing the Shelf Life of Tilapia Surimi with Mosambi Peel Extract: Protection against Oxidative, Structural, and Textural Degradation during Frozen Storage

“…The present results showed that TBA increment in MPE samples was significantly lower over the storage period, which was 43.56% less than that of the control (818 ± 0.003 mg-malonaldehyde/kg) at the end of the storage period, which indicates that MPE could effectively retard lipid oxidation during frozen storage because of its high antioxidant activity. The result of this study is also supported by some previous studies, wherein it was reported that polyphenolic extracts from clove buds, thinned young apples, and grape seed extract showed strong antioxidant effects and significantly restrained increases of TBA in surimi. − …”

Stable chlorine dioxide combined with slightly acidic electrolyzed water: an effective way to delay protein deterioration of refrigerated large yellow croaker (Pseudosciaena crocea)

“…α‐helix and β‐sheet can manifest protein conformation, while its looseness degree can be demonstrated by a random coil and β‐turn (Lan et al ., 2022a). The fresh samples contained 28.37% β‐sheet, 15.18% random coil, 32.29% α‐helix, and 24.16% β‐turn.…”

“…The total ratio of α‐helices and β‐sheets (more organised secondary structures) in samples treated by ClO 2 , SAEW, and ClO 2 combined with SAEW (53.98–59.65%) was significantly increased at day 12 compared to that in CK group (46.10%). In addition, on the 12th day, samples in CK group formed a slightly loose conformation, which was caused by a larger proportion of random coils (22.11%) than those in other groups (15.24–21.57%) (Lan et al ., 2022a). At day 12, the total ratio of α‐helices and β‐sheets in ClO 2 + SAEW group was higher than that in ClO 2 or SAEW group, reaching 59.65%, with the lowest ratio of (15.24%), resulting in a more regular secondary structure.…”

Covalent and non‐covalent interaction of myofibrillar protein and cyanidin‐3‐O‐glucoside: focus on structure, binding sites and in vitro digestion properties

“…The method for performing SDS–PAGE was based on a method reported previously 15 . The diluted MP solution (2 mg·mL ‐1 ) was mixed with a quarter of its volume of SDS‐PAGE sample loading buffer with or without β ‐mercaptoethanol ( β ‐ME).…”

“…The method for performing SDS-PAGE was based on a method reported previously. 15 The diluted MP solution (2 mg•mL -1 ) was mixed with a quarter of its volume of SDS-PAGE sample loading buffer with or without ⊎-mercaptoethanol (⊎-ME). After heating at 90 °C for 3 min, 10 μL samples were added to a 5% stacking gel and 12% separating gel.…”