The effects on in vitro digestibility from different developmental stages of silkworm larvae, Bombyx mori (Lepidoptera: Bombycidae) and position of mulberry leaves, Morus alba (Rosales: Moraceae)

Chandang, Pipatpong; Thongprajukaew, Karun; Chotimanothum, Banthari; Kovitvadhi, Attawit; Kovitvadhi, Uthaiwan; Pakkong, Pannee

doi:10.1016/j.aspen.2017.08.005

Cited by 10 publications

(26 citation statements)

References 17 publications

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“…Extraction of crude digestive enzymes was performed as described by Chandang et al (2017). Briefly, the whole body of the larvae was extracted in 200 mM phosphate buffer (pH 8) (1:2 w/v) using a micro-homogenizer (TH 02, Omni International, Marietta, USA).…”

Section: Enzyme Extractionmentioning

confidence: 99%

“…The optimal conditions for each enzyme assay were chosen from our previous study on mulberry silkworm larvae (Chandang et al, 2017). Cellulase activity (EC 3.2.1.4) was assayed according to Vatanparast et al (2014) using carboxymethyl cellulose (CMC) as the substrate.…”

Section: Activities Of Cellulase Amylase and Trypsinmentioning

confidence: 99%

“…However, the leaves contain high amounts of the polymer building blocks of lignocellulosic materials (LCM), i.e. 14-28% lignin and 12-36% cellulose (Al-Kirshi et al, 2013;Chandang et al, 2017) on a dry matter basis, which reduce the efficiency of feed utilization in silkworms by physically protecting the carbohydrate and protein in the cell content of the leaves from enzymatic hydrolysis.…”

Section: Introductionmentioning

confidence: 99%

“…LCM content varies with leaf position. Generally, mature mulberry leaves contain a higher portion of LCM (17.3-18.85%) than younger leaves (14.4-14.6%) (Chandang et al, 2017). Therefore, the appearance of LCM may interfere with silkworm digestive enzymes since it acts as nonspecific enzyme inhibitor.…”

Section: Introductionmentioning

confidence: 99%

“…However, positive findings by lowering LCM and increasing amount of nitrogen-free extract (NFE) have been observed by a number of authors (Bamidele & Akanbi, 2013;Bhat et al, 2009;Tresina & Mohan, 2011). This change provides desirable characteristics in mulberry leaves since young silkworms require nutrients for energy rather than protein (Chandang et al, 2017), and the younger stage of larvae has a higher carbohydrate metabolism relative to older instars (B. Chen et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Improving the nutritive value of mulberry leaves, Morus spp. (Rosales: Moraceae) for silkworm larvae, Bombyx mori (Lepidoptera: Bombycidae) using gamma irradiation

Chundang

Thongprajukaew

Kovitvadhi

et al. 2020

Journal of Radiation Research and Applied Sciences

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The nutritive value and in vitro digestibility using the digestive enzyme extracts from different stages of silkworm larvae (Bombyx mori) were evaluated in different leaf positions (1, 2, 3, 4, or 5) of mulberry leaves (Morus spp.) with or without gamma irradiation treatment (native vs. irradiated). At least one of studied factors and their interaction had effects on the overall proximate composition and physicochemical properties (p < 0.05), in terms of water absorption index, water solubility, swelling power, pH, relative crystallinity, and FT-IR spectra. Cellulase activity was significantly decreased with larval stage, while a similar pattern was observed for amylase activity, but with relatively low activity in the first stage. The highest trypsin activity was observed in the fourth stage, followed by the second and fifth stages. These changes across the five larval stages could have significant effects on the in vitro digestibility of protein (IVPD) and carbohydrate (IVCD), as well as the effect of leaf position on IVCD. However, significant improvements in IVPD and IVCD were obtained for irradiated leaves relative to the control native leaves, independent of all studied factors.

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Section: Enzyme Extractionmentioning

confidence: 99%

Section: Activities Of Cellulase Amylase and Trypsinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%