The effects on in-vitro fertilization of autoantibodies to spermatozoa in subfertile men

Rajah, Shantil V.; Parslow, J. M.; Howell, R. J. S.; Hendry, W. F.

doi:10.1093/oxfordjournals.humrep.a138196

Cited by 64 publications

(36 citation statements)

References 9 publications

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“…Antisperm antibodies may have heterogeneous effects, depending on their cognate antigens [40]. They have been found to affect sperm motility [6,19], the acrosome reaction [8], penetration of cervical mucus [14], sperm binding to the zona pellucida [18], and sperm-egg fusion [9,42]. In addition, in the present study, sperm granulomas were histologically observed in 4 of 35 rats, and degenerated spermatids were found within the lumen of the cauda epididymis.…”

Section: Discussionsupporting

confidence: 48%

Effect of Short Period Vasectomy on FSH, LH, Inhibin and Testosterone Secretions, and Sperm Motility in Adult Male Rats

et al. 2011

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The present study was undertaken to clarify changes in secretions of FSH, LH, inhibin and testosterone, and sperm motility after bilateral vasectomy in adult male rats. Bilateral vasectomy was created surgically (treated group) and intact rats were used as control (control group). On days 3,5,7,14,30, 60, and 90 after surgery, plasma concentrations of FSH, LH, inhibin, and testosterone were measured by radioimmunoassay, and sperm motility characteristics were measured by computer-assisted sperm analysis (CASA). The results show that weights of epididymides significantly increased in vasectomized rats as compared to control rats. Histologically, damage to spermatogenesis was observed in vasectomized rats. Multinucleated giant cells were observed in the lumen of some seminiferous tubules, and there were degenerative spermatids in the epididymides of vasectomized rats. Plasma levels of LH, FSH, and testosterone only decreased on day 3 after vasectomy; however, plasma levels of ir-inhibin significantly increased on day 3 after vasectomy. In addition, the sperm motility parameters, straight-line velocity, curvilinear velocity, deviation of the sperm head from the mean trajectory and the maximum amplitude of lateral head displacement were decreased from day 60 after vasectomy. These results suggest that vasectomy reduces sperm motility starting from day 60 after vasectomy, and early bilateral vasectomy does not strongly affect the endocrine function of the testis, though it may result in damage to spermatogenesis in vasectomized rats.

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Section: Discussionsupporting

confidence: 48%

Effect of Short Period Vasectomy on FSH, LH, Inhibin and Testosterone Secretions, and Sperm Motility in Adult Male Rats

et al. 2011

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“…6,29 Conventional in vitro fertilization (IVF) usually results in lower fertilization and cleavage rates in cases involving ASA-mediated infertility. 10,11,21,30 Intracytoplasmic sperm injection, on the other hand, has been shown to successfully overcome any deleterious effects of ASA. ICSI has yielded excellent results irrespective of the ASA levels, and results in fertilization rates, embryo development, and pregnancy rates similar to that which has been observed in other categories of male infertility.…”

Section: B Discussionmentioning

confidence: 99%

Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane

Schneider¹,

Feijó²,

Verza³

et al. 2014

Medical Express

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OBJECTIVE:We evaluated the effectiveness of sperm washing using the discontinuous two-layer density gradient centrifugation method to remove antisperm antibodies attached to the sperm surface. METHOD: We prospectively enrolled sixty-six men with unexplained infertility who were seeking evaluation. Each patient delivered one semen specimen for the study. We determined antisperm antibody levels using the direct immunobeads test. Specimens were classified into two groups according to the pre-washing levels of antibody-bound spermatozoa: group 1 (low antisperm antibodies levels, immunobeads test , 20%; n ¼ 54), and group 2 (high antisperm antibodies levels, immunobeads test $ 20%; n ¼ 12). Sperm washing was carried out using the discontinuous two-layer colloidal density gradient centrifugation method. Pre-and post-wash levels of antisperm antibodies were compared in the groups. RESULTS: The pre-and post-wash percentage of spermatozoa with antisperm antibodies attached to their surface was 11% and 6.5% in group 1 (mean difference ¼ 40%; p , 0.01), and 30% and 19.5% in group 2 (mean difference ¼ 36.8%, p ¼ 0.02), respectively. The effectiveness of density gradient centrifugation in removing antisperm antibodies was not different between the groups, but individual variations from 252.3% to 23.9% were observed. CONCLUSIONS: Sperm washing by density gradient centrifugation is an overall effective method to remove antibodies bound to sperm membranes, regardless of the levels of antisperm antibodies in the neat semen. Due to an inter-individual variation in the effectiveness of the method, we recommend that each patient be tested before applying sperm washing by density gradient centrifugation in intrauterine insemination.

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“…Indeed, when the degree of sperm autoimmunization was taken into account, it was always inversely correlated with the overall fertilization rate (130-132, and table 2). But, noteworthy, in some individual patients, a high fertilization rate was achieved even in the presence of a high degree of sperm autoimmunization (121,123,124,128).…”

Section: In Vitro Fertilization (Ivf) As Model Of Studymentioning

confidence: 99%

“…Table 2 shows the fertilization rates reported in series including couples with sperm autoimmunization in the male partner. In most reports the fertilization rate was significantly lower in the presence of sperm-bound antibodies than in the case of other indications for IVF (119)(120)(121)(122)(123)(124). However, in some other reports no significant difference was found (125-127).…”

Section: Interference With the Fertilization Processmentioning

confidence: 99%

“…Only in some reports the conventional semen parameters were taken into account in the comparison between patients with and without ASA (121,122,124,131). In these series, an independent impairment by sperm-bound antibodies was reported in the presence of normal semen parameters (121,124,131) as well as in the presence of asthenozoospermia (131) or teratozoospermia (122). Secondly, the criteria employed to define the occurrence of immunological infertility were different among series, and often inadequate.…”

Section: Interference With the Fertilization Processmentioning

confidence: 99%

See 1 more Smart Citation

Naturally-occurring antisperm antibodies in men: interference with fertility and implications for treatment

Francavilla¹

1999

Front Biosci

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The effects on in-vitro fertilization of autoantibodies to spermatozoa in subfertile men

Cited by 64 publications

References 9 publications

Effect of Short Period Vasectomy on FSH, LH, Inhibin and Testosterone Secretions, and Sperm Motility in Adult Male Rats

Effect of Short Period Vasectomy on FSH, LH, Inhibin and Testosterone Secretions, and Sperm Motility in Adult Male Rats

Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane

Naturally-occurring antisperm antibodies in men: interference with fertility and implications for treatment

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