The effects on oxidative DNA damage of lapa roscopic gastric band applications in morbidly obese patients

Abstract

Background: Obesity may induce oxidative stress, causing oxidative damage of DNA. We examined associations between decreasing serum and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels and weight loss in morbidly obese patients before and 6 months after laparoscopic adjustable gastric banding (LAGB). Methods:We compared patients who had surgery for morbid obesity with healthy, nonobese controls. Urine and fasting blood samples were collected once from the controls and from the morbidly obese patients before and 6 months after the LAGB. The serum and urinary 8-OHdG levels were evaluated in these groups using an enzyme-linked immunosorbent assay kit. Results:We included 20 patients who had surgery for morbid obesity (8 men, 12 women, mean body mass index [BMI] 46.82 ± 4.47) and 20 healthy, nonobese people (10 men, 10 women, mean BMI 22.52 ± 2.08) in our study. There was no significant difference in serum 8-OHdG levels between the groups, whereas urinary 8-OHdG levels were significantly higher in morbidly obese patients than in controls. Weight, BMI and serum and urinary 8-OHdG levels were significantly decreased in morbidly obese patients 6 months after LAGB. Conclusion:The LAGB provides efficient weight loss in patients with morbid obesity. The systemic oxidative DNA damage was increased by the morbid obesity, but this increase was not related to weight gain, and it was more evident in serum than urine samples. After LAGB for morbid obesity, the oxidative DNA damage declined both in serum and urine.

Gene polymorphisms and increased DNA damage in morbidly obese women

“…Similarly, high levels of 8-OHdG were detected in the skeletal muscle of overweight patients, suggesting obesity as a contributing factor to increased oxidative stress [51]. In addition, a recent study suggested that DNA damage caused by ROS occurs more frequently in morbidly obese patients than in age-matched, healthy non-obese controls [48].…”

“…It is well known that oxidative stress can lead to oxidative DNA damage, causing strand breaks, altered gene expression and ultimately mutagenesis [48]. A recent study showed that both lipid peroxidation and 8-OHdG (8-hydroxydeoxyguanosine, a biomarker for oxidative damage) were positively correlated with BMI, blood pressure, waist/hip circumference and C-reactive protein [49].…”

Oxidative damage to DNA in independent-living elderly persons and their correlations with sociodemographic, anthropometric, and functional parameters

“…In addition, data from literature shows that rapid weight loss followed by surgeries for the introduction of gastric banding resulted in a decline in 8-OHdG concentrations excreted in the urine of patients undergoing this procedure 11 .…”

“…Although literature requires reviews that directly address the issue of level of education and oxidative stress, different authors have shown a positive correlation between low levels of schooling, smoking, a sedentary lifestyle, low consumption of antioxidant foods, and high levels of oxidative stress 19,11 .…”

“…A study by Kocael et al found that serum levels of 8-OHdG in patients with morbid obesity declined after surgery for the implantation of a gastric band, evidencing a link between obesity and oxidative DNA damage 11 .…”