The efficacy and neuroprotective effects of edaravone-loaded mPEG-b-PLGA polymeric nanoparticles on human neuroblastoma SH-SY5Y cell line as in vitro model of ischemia

Sharifyrad, Motahare; Gohari, Sepehr; Fathi, Mojtaba; Danafar, Hossein; Hosseini, Mir-Jamal; Mostafavi, Hossein; Manjili, Hamidreza Kheiri

doi:10.1016/j.jddst.2022.103378

Cited by 7 publications

(5 citation statements)

References 58 publications

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“…GSH-PMAA-EDV has not induced any toxic histopathological adverse effect in CA1 site. In our previous study, mPEG-b-PLGA EDV nanoparticles also have not induced any toxicity in human neuroblastoma cell line while suppressing the expression of antiapoptotic genes [ 42 ]. The edaravone dosage used in this study was 100 times less than that of the calculated equal dose for rats, yet showing effectiveness and inducing no toxicity.…”

Section: Discussionmentioning

confidence: 99%

Enhancing the Neuroprotection Potential of Edaravone in Transient Global Ischemia Treatment with Glutathione- (GSH-) Conjugated Poly(methacrylic acid) Nanogel as a Promising Carrier for Targeted Brain Drug Delivery

Mozafari

Rashidzadeh

Bijani

et al. 2023

Oxidative Medicine and Cellular Longevity

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Ischemic stroke is the most common among various stroke types and the second leading cause of death, worldwide. Edaravone (EDV) is one of the cardinal antioxidants that is capable of scavenging reactive oxygen species, especially hydroxyl molecules, and has been already used for ischemic stroke treatment. However, poor water solubility, low stability, and bioavailability in aqueous media are major EDV drawbacks. Thus, to overcome the aforementioned drawbacks, nanogel was exploited as a drug carrier of EDV. Furthermore, decorating the nanogel surface with glutathione as targeting ligands would potentiate the therapeutic efficacy. Nanovehicle characterization was assessed with various analytical techniques. Size (199 nm, hydrodynamic diameter) and zeta potential (-25 mV) of optimum formulation were assessed. The outcome demonstrated a diameter of around 100 nm, sphere shape, and homogenous morphology. Encapsulation efficiency and drug loading were determined to be 99.9% and 37.5%, respectively. In vitro drug release profile depicted a sustained release process. EDV and glutathione presence in one vehicle simultaneously made the possibility of antioxidant effects on the brain in specific doses, which resulted in elevated spatial memory and learning along with cognitive function in Wistar rats. In addition, significantly lower MDA and PCO and higher levels of neural GSH and antioxidant levels were observed, while histopathological improvement was approved. The developed nanogel can be a suited vehicle for drug delivery of EDV to the brain and improve ischemia-induced oxidative stress cell damage.

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Section: Discussionmentioning

confidence: 99%

Enhancing the Neuroprotection Potential of Edaravone in Transient Global Ischemia Treatment with Glutathione- (GSH-) Conjugated Poly(methacrylic acid) Nanogel as a Promising Carrier for Targeted Brain Drug Delivery

Mozafari

Rashidzadeh

Bijani

et al. 2023

Oxidative Medicine and Cellular Longevity

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“…Sharifyrad et al used the human neuroblastoma SH-SY5Y cell line to create an in vitro ischemia model. In this model, edaravone micelles were more effective than free edaravone at lowering the lactate dehydrogenase (LDH), nitric oxide (NO) activity, intracellular reactive oxygen species (ROS), cell apoptosis rate, and pro-apoptotic gene (Bax) expression and raising anti-apoptotic gene (HSP70 and Bcl-2) expression [ 67 ]. Several traditional Chinese remedies or their components possess anti-oxidation and anti-apoptosis properties, which are advantageous in the treatment of ischemic cerebrovascular disorders.…”

Section: Applications Of Plga-based Nanostructures In Drug Delivery A...mentioning

confidence: 99%

The Recent Applications of PLGA-Based Nanostructures for Ischemic Stroke

Yan,

Huang,

Feng

et al. 2023

Pharmaceutics

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With the accelerated development of nanotechnology in recent years, nanomaterials have become increasingly prevalent in the medical field. The poly (lactic acid–glycolic acid) copolymer (PLGA) is one of the most commonly used biodegradable polymers. It is biocompatible and can be fabricated into various nanostructures, depending on requirements. Ischemic stroke is a common, disabling, and fatal illness that burdens society. There is a need for further improvement in the diagnosis and treatment of this disease. PLGA-based nanostructures can facilitate therapeutic compounds’ passage through the physicochemical barrier. They further provide both sustained and controlled release of therapeutic compounds when loaded with drugs for the treatment of ischemic stroke. The clinical significance and potential of PLGA-based nanostructures can also be seen in their applications in cell transplantation and imaging diagnostics of ischemic stroke. This paper summarizes the synthesis and properties of PLGA and reviews in detail the recent applications of PLGA-based nanostructures for drug delivery, disease therapy, cell transplantation, and the imaging diagnosis of ischemic stroke.

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“…[12,13] In general, PLGA is a biodegradable synthetic polymer and is readily able to be metabolized through the Krebs cycle. [14] Moreover, due to its complete biocompatibility, high drug loading capacity, drug site-specific targeting, and sustained drug release by scheduling the degradation time, it is considerably used in a great number of drug delivery systems. [15,16] Curcumin (CUR), the natural phenolic organic compound derived from the dried rhizomes of Curcuma longa, is the main ingredient in turmeric.…”

Section: Introductionmentioning

confidence: 99%

Curcumin‐Encapsulated Poly(lactic‐co‐glycolic acid) Nanoparticles: A Comparison of Drug Release Kinetics from Particles Prepared via Electrospray and Nanoprecipitation

Roshan,

Haddadi‐Asl,

Ahmadi

et al. 2024

Macro Materials & Eng

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Controlled drug release (CDR) is a significant field of research in medical sciences due to its numerous clinical advantages over traditional methods. Encapsulation of a drug in a polymeric matrix is common technique to achieve CDR. In this study, drug‐polymer particles are prepared using poly(lactic‐co‐glycolic acid) (PLGA) as the polymer and curcumin (CUR) as model drug. Two different methods, electrospray and nanoprecipitation, are used to prepare the particles, and optimal samples in each process are selected based on size and polydispersity index (PDI). Samples are characterized using various tests, and entrapment efficiency (EE%) and drug loading (DL%) are calculated using UV spectroscopy. The results showed that nanoprecipitated and electrosprayed PLGA particles successfully encapsulated CUR, with higher encapsulation efficiency (93.2%) and loading capacity (7.2%) for electrosprayed particles. The in vitro drug release showed that electrospray particles have a slower release rate due to higher encapsulation efficiency. The electrospray method turned out to be more viable for synthesizing these polymer‐drug particles due to smaller particle size, lower PDI, higher entrapment efficiency, and drug loading percentage. Finally, the antibacterial behavior of the particles proved that prepared particles provide excellent antibacterial efficacy (99.9%) and can be used as drug delivery systems.

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The efficacy and neuroprotective effects of edaravone-loaded mPEG-b-PLGA polymeric nanoparticles on human neuroblastoma SH-SY5Y cell line as in vitro model of ischemia

Cited by 7 publications

References 58 publications

Enhancing the Neuroprotection Potential of Edaravone in Transient Global Ischemia Treatment with Glutathione- (GSH-) Conjugated Poly(methacrylic acid) Nanogel as a Promising Carrier for Targeted Brain Drug Delivery

Enhancing the Neuroprotection Potential of Edaravone in Transient Global Ischemia Treatment with Glutathione- (GSH-) Conjugated Poly(methacrylic acid) Nanogel as a Promising Carrier for Targeted Brain Drug Delivery

The Recent Applications of PLGA-Based Nanostructures for Ischemic Stroke

Curcumin‐Encapsulated Poly(lactic‐co‐glycolic acid) Nanoparticles: A Comparison of Drug Release Kinetics from Particles Prepared via Electrospray and Nanoprecipitation

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