The efficacy of photodynamic therapy and sodium hypochlorite in root canal disinfection by a single-file instrumentation technique

Oliveira, Bruna Paloma de; Aguiar, Carlos; Câmara, Andréa Cruz; Albuquerque, Manoel Bandeira de; Correia, Ana Cristina Regis de Barros; Soares, Mônica Felts de La Roca

doi:10.1016/j.pdpdt.2015.05.004

Cited by 34 publications

(33 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The study demonstrated relatively similar results, as the lowest survival rate was observed in the group of combined PDT with NaOCl. However, the survival rates were relatively lower in the 5.25% NaOCl group, PDT group, and combined PDT with NaOCl group when compared to the survival rates of our study groups [23].…”

Section: Discussioncontrasting

confidence: 76%

“…The heterogeneity of outcomes may be due to the presence of several sensitive factors in the PDT protocol that may affect the outcomes, including the type of PS, its concentration, its pre-irradiation time, the laser's wavelength, the irradiance time, the type of irrigant, its exposure time, the bacterial biofilms, and the utilized microbiological technique to evaluate the colony counts [14,15,23].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Photodynamic Therapy Using a Diode Laser 635 nm as an Adjunct to Conventional Chemo-Mechanical Endodontic Procedures against Enterococcus faecalis Biofilm: Ex-Vivo Study

et al. 2020

View full text Add to dashboard Cite

The study aimed to evaluate the antimicrobial effect of photodynamic therapy (PDT) with the use of Toluidine Blue (TB) on extracted teeth infected with biofilms of Enterococcus faecalis. Fifty-four extracted teeth with single-roots and single canals were mechanically shaped, autoclaved, and contaminated with E. faecalis. They were randomly divided into six groups: two groups were negative and positive control groups, two groups were subjected to mechanical instrumentation and PDT with different pre-irradiation times and irradiation times, and two groups were subjected to chemo-mechanical endodontic treatment and PDT with different pre-irradiation times and irradiation times. In PDT groups, after the application of TB, the canals were irradiated with a diode laser of wavelength 635 nm, with a fiber diameter of 200 μm and 100 mW of power in continuous mode. The bacterial load was evaluated using a BioTimer Assay protocol. The greatest reduction of bacterial load was observed in groups of combined PDT with chemo-mechanical treatment. The reductions of bacterial load in groups of combined PDT with chemo-mechanical treatment, and in the positive control group, were significant (p < 0.01) when compared to that of the negative control group. Photodynamic therapy as an adjunctive modality may improve the disinfection capacity of conventional endodontic treatment against E. faecalis.

show abstract

Section: Discussioncontrasting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Evaluation of Photodynamic Therapy Using a Diode Laser 635 nm as an Adjunct to Conventional Chemo-Mechanical Endodontic Procedures against Enterococcus faecalis Biofilm: Ex-Vivo Study

et al. 2020

View full text Add to dashboard Cite

show abstract

“…6,7 Evidence shows that fungi are often present in infections resistant to conventional RCT and are partially responsible for treatment failure of PA lesions despite the debridement and irrigation of the root canal system. 8 Candida albicans and some other microorganisms have been isolated from root canals of teeth with and without PA lesions. 9 Many studies reported that these organisms can resist the antibacterial effect of calcium hydroxide.…”

Section: Comparison Of the Antimicrobial Efficacy Of Calcium Hydroxidmentioning

confidence: 99%

Comparison of the Antimicrobial Efficacy of Calcium Hydroxide and Photodynamic Therapy Against Enterococcus faecalis and Candida albicans in Teeth With Periapical Lesions; An In Vivo Study

et al. 2017

View full text Add to dashboard Cite

IntroductionMicrobial infection plays an important role in the development of periapical (PA) lesions.1 Elimination of the pathological microflora of the root canal system is a major goal in endodontic treatment. Inadequate disinfection of the root canal system may lead to treatment failure and development of PA lesions.2 Killing the bacteria of root canal system is not always achievable with current root canal treatment (RCT) techniques (mechanical debridement associated with chemical irrigation).3,4 The anatomical complexity of the root canal system makes it almost impossible to completely eliminate the bacteria using conventional mechanical and chemical techniques, even with the highest technical standards. 5A 94% treatment success rate has been reported in cases with a negative culture before the obturation of root canal system. This rate decreases to 68% in cases undergoing root canal filling despite the cultures being positive. There is a challenge for the clinician in treatment of teeth with PA lesions, all efforts and attempts have been made to eliminate irritating agents from the root canal system in order to provide healing in the periradicular tissues. Methods: This in vivo study was conducted on 20 patients with single-rooted mandibular premolar with previously failed endodontic treatment. This study was performed as a clinical trial (IRCTID: IRCT2016090429686N1). After conventional chemo-mechanical root canal preparation (hand and rotary instruments and 2.5% NaOCl), microbiological samples were obtained using sterile paper points, then stored in thioglycolate solution and transferred to a microbiology laboratory. Group 1 (n = 10) specimens underwent aPDT (diode laser 808 nm + 50 mg/mL methylene blue), while creamy calcium hydroxide paste was used in group 2 for a duration of 1 week. A control sample was taken with sterile paper points and F3 Protaper rotary file. The samples were dispersed in transport medium, serially diluted, and cultured on selective mediums to determine the number of colony forming units (CFUs). Data were analyzed by Mann-Whitney U test at 5% significance level. The significance level for all analyses was set at P < 0.05. Results: Number of CFU significantly decreased in both groups after the interventions (P < 0.001); however, there was no significant difference in the colony count between the 2 groups. Conclusion: aPDT and calcium hydroxide therapy showed the same antimicrobial efficacy on E. faecalis and C. albicans.

show abstract

“…2 In recent years, photodynamic therapy (PDT) has been shown to be a promising antimicrobial therapy capable of eliminating microorganisms presenting in endodontic infections. 3,4 This treatment is based on the interaction of three components: a photosensitizer dye, a light source and oxygen. The mechanism of action of PDT occurs when the photosensitizer absorbs photons from the light source, and their electrons go into an excited state.…”

Section: Introductionmentioning

confidence: 99%

“…Upon returning to its basic state, the photosensitizer transfers energy to a substrate, such as oxygen, forming free radicals of high cytotoxicity, which can cause the death of microorganisms. 5 Several in vitro 4 and in vivo 6 studies have demonstrated that PDT is presented as an important antimicrobial tool for conventional endodontic treatment. Nevertheless, to date, only a few investigations 7 have evaluated the use of this therapy in surgical endodontic treatment.…”

Section: Introductionmentioning

confidence: 99%

Photodynamic Therapy: A Novel Ally for Surgical Endodontic Treatment? Case Report

Oliveira

Câmara

Ferreira³

et al. 2018

J Lasers Med Sci

Self Cite

View full text Add to dashboard Cite

Introduction: Photodynamic therapy (PDT) has emerged as a powerful tool for conventional endodontic treatment, capable of eradicating microorganisms present in endodontic infections. Despite this, the use of PDT in the surgical endodontic treatment is not well-known. Case Report: This paper describes the case of a patient with asymptomatic apical periodontitis in a mandibular incisor submitted to surgical endodontic treatment associated with PDT. After conventional procedures, photosensitizer methylene blue (0.01%) was applied to the retrograde cavity for 3 minutes, followed by irradiation with diode laser (100 mW and 660 nm) for 3 minutes. Six months after the procedure, the patient was asymptomatic, and the radiographic examination showed healthy periradicular tissues. Conclusion: The association of PDT with surgical endodontic treatment was effective, suggesting that this therapy may provide additional benefits to patients when compared to the conventional surgical technique.

show abstract

The efficacy of photodynamic therapy and sodium hypochlorite in root canal disinfection by a single-file instrumentation technique

Cited by 34 publications

References 45 publications

Evaluation of Photodynamic Therapy Using a Diode Laser 635 nm as an Adjunct to Conventional Chemo-Mechanical Endodontic Procedures against Enterococcus faecalis Biofilm: Ex-Vivo Study

Evaluation of Photodynamic Therapy Using a Diode Laser 635 nm as an Adjunct to Conventional Chemo-Mechanical Endodontic Procedures against Enterococcus faecalis Biofilm: Ex-Vivo Study

Comparison of the Antimicrobial Efficacy of Calcium Hydroxide and Photodynamic Therapy Against Enterococcus faecalis and Candida albicans in Teeth With Periapical Lesions; An In Vivo Study

Photodynamic Therapy: A Novel Ally for Surgical Endodontic Treatment? Case Report

Contact Info

Product

Resources

About