The Efficiency of Bone Marrow Aspiration for the Harvest of Connective Tissue Progenitors from the Human Iliac Crest

Abstract

Bone marrow aspiration is an effective means of harvesting CTP-Os but is associated with dilution with peripheral blood. Overall, we found that 63.5% of all CTP-Os within iliac cancellous bone resided on the trabecular surface; however, 48% of the patients had more CTP-Os contributed by the IC-MS than the IC-TS fraction.

Analysis of Time to Form Colony Units for Connective Tissue Progenitor Cells (Stem Cells) Harvested From Concentrated Bone Marrow Aspirate and Subacromial Bursa Tissue in Patients Undergoing Rotator Cuff Repair

“…A colony was defined as a group of 8 or more cells in a culture. 21 , 22 CTPs must meet 3 criteria: (1) express surface markers CD 73, CD90, and CD105; (2) differentiate into fat, bone, or cartilage; and (3) adhere to tissue culture plastic. 21 Once colonies were identified, dishes were systematically examined at 10× magnification to determine total number of colonies (CFU) present ( Fig 1 ).…”

“… 21 Once a group of cells has formed in a unit together (8 cells), one colony has formed. 21 , 22 , 23 The number of CFU fibroblasts per volume in a given sample of harvested CTPs has been described as a measurable factor impacting CTP “quality” previously in the orthopaedic literature. 24 Although CFU count has been described as a metric to assess CTPs, there is still significant debate regarding optimal in vitro determinants of in vivo CTP viability.…”

Proceedings of the signature series symposium “cellular therapies for orthopaedics and musculoskeletal disease proven and unproven therapies—promise, facts and fantasy,” international society for cellular therapies, montreal, canada, may 2, 2018

“…The hierarchy of stem and progenitor populations in BM began with Friedenstein and Owen and others [109,122À125]. CTPs with different biological properties can be found in the marrow space and the trabecular surface [41]. Some CTPs are derived from aspirates of BM that retain hyaluronan (HA) on their surface, suggesting that they are derived from a niche characterized by an HA-rich extracellular matrix [43].…”

“…The prevalence of CTPs in native tissues varies widely from patient to patient and from tissue to tissue, from a mean of nearly one in 20 000 in native BM to one in 2000 in healthy fat, synovium and cartilage [41,89,129,130]. CTPs, even in low numbers, represent an available target cell population, with biological potential that may be elicited or induced by local or systemic drug delivery or local bioactive biomaterials [4].…”

Cell therapy for orofacial bone regeneration: A systematic review and meta‐analysis

“…Bone marrow is known to contain a heterogeneous population of progenitor cells (including MSCs, HSCs, and endothelial progenitor cells), and supporting growth factors and cytokines (Chahla et al., ; Patterson et al., ). Concentration of the mononuclear cell fraction (MNC) of bone marrow (which includes MSCs) via density gradient centrifugation steps to remove red blood cells, granulocytes, immature myeloid precursors, and platelets, represents an attractive clinical strategy, since it is currently FDA‐approved and shown to be efficacious as a point‐of‐care method of autologous cell delivery (Chahla et al., ; Jager et al., ).…”