The electrical pulse application enhances intra-cellular localization and potentiates cytotoxicity of curcumin in breast cancer cells

Bazzolo, Bianca; Mittal, Lakshya; Sieni, Elisabetta; Piovan, Anna; Filippini, Raffaella; Conconi, Maria Teresa; Camarillo, Ignacio G.; Sundararajan, Raji

doi:10.1016/j.bioelechem.2021.107817

Cited by 5 publications

(2 citation statements)

References 54 publications

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“…Reintgen et al observed that basal cell carcinoma had an obvious response to EIC which could greatly reduce the dose of chemotherapy drugs [ 15 ]. In 2021, Sundararajan et al found that electrical impulses could increase the cell uptake of curcumin and thus enhanced the drug toxicity to cancer cells [ 16 ]. EIC has unique advantages in cancer therapy, which can reach the targeted tissue and make it easier to operate [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Self-Powered Electrical Impulse Chemotherapy for Oral Squamous Cell Carcinoma

et al. 2022

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Oral squamous cell carcinoma (OSCC) is a common oral cancer of the head and neck, which causes tremendous physical and mental pain to people. Traditional chemotherapy usually results in drug resistance and side effects, affecting the therapy process. In this study, a self-powered electrical impulse chemotherapy (EIC) method based on a portable triboelectric nanogenerator (TENG) was established for OSCC therapy. A common chemotherapeutic drug, doxorubicin (DOX), was used in the experiment. The TENG designed with zigzag structure had a small size of 6 cm × 6 cm, which could controllably generate the fixed output of 200 V, 400 V and 600 V. The electrical impulses generated by the TENG increased the cell endocytosis of DOX remarkably. Besides, a simply and ingeniously designed microneedle electrode increased the intensity of electric field (EF) between two adjacent microneedle tips compared with the most used planar interdigital electrode at the same height, which was more suitable for three-dimensional (3D) cells or tissues. Based on the TENG, microneedle electrode and DOX, the self-powered EIC system demonstrated a maximal apoptotic cell ratio of 22.47% and a minimum relative 3D multicellular tumor sphere (MCTS) volume of 160% with the drug dosage of 1 μg mL−1.

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Section: Introductionmentioning

confidence: 99%

Self-Powered Electrical Impulse Chemotherapy for Oral Squamous Cell Carcinoma

et al. 2022

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“…Breast cancer (BC) is the most common cancer and the fifth leading cause of cancer deaths worldwide [1]. Although the mortality rate of BC has decreased due to the development of treatment methods such as surgery, radiation, chemotherapy, endocrine therapy, and targeted therapy, systemic treatment to prevent metastasis is less effective [2].…”

Section: Introductionmentioning

confidence: 99%

A Novel Necroptosis-Related miRNA Signature for Predicting the Prognosis of Breast Cancer Metastasis

Wang

Jiang

et al. 2022

Disease Markers

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Objective. Necroptosis was recently identified as a form of programmed cell death that plays an essential role in breast cancer metastasis. MicroRNAs (miRNAs) have long been recognized to affect cell death and tumor growth. In this study, we aimed to screen for necroptosis-associated miRNAs that predict breast cancer metastasis. Method. This study used The Cancer Genome Atlas (TCGA) public database to obtain miRNA expression data and associated clinical data from breast cancer patients and then retrieved miRNA data related to necrosis and apoptosis. Next, using Cox regression model analysis (univariate or multivariate) as well as a comparison analysis (differential analysis), a prognostic multi-miRNA molecular marker was established. Finally, prognosis-related miRNAs were utilized to identify target genes, and the functions of the target genes were analyzed for enrichment to investigate the probable mechanisms of the miRNAs. Results. Ten miRNAs were screened through differential analysis to build models: hsa-miR-148a-3p, hsa-miR-223-3p, hsa-miR-331-3p, has-miR-181a-5p, hsa-miR-181b-5p, hsa-miR-181c-5p, hsa-miR-181d-5p, hsa-miR-200a-5p, hsa-miR-141-3p, and hsa-miR-425-5p. The multivariate Cox regression model was an independent prognostic factor (univariate Cox regression results: HR = 3.2642 , 95 % CI = 1.5773 − 6.7554 , P = 0.0014 ; multivariate Cox regression results: HR = 3.1578 , 95 % CI = 1.5083 − 6 , P = 0.0023 ). The survival curve of the risk score also revealed that patients with a high risk score had a poor prognosis ( P = 2 e − 04 ). The receiver operating characteristic (ROC) curve showed that the model has a certain prediction ability. Batch survival analysis of the miRNAs in the model was conducted and showed that hsa-miR-331-3p ( P = 0.0182 ) was strongly associated with prognosis. Twenty-three predicted target genes were obtained, and Gene Ontology (GO) enrichment analysis showed that these target genes were strongly enriched in transcriptional initiation and cell membrane trafficking. Conclusion. Our research identified a novel miRNA marker for predicting breast cancer patient prognosis and lays the groundwork for future research on necroptosis-related genes.

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