The electrophoretic pattern of normal human gastric juice and of the gastric juice of patients with gastric ulcer and gastric cancer

Piper, D. W.; Stiel, Mirjam C.; Builder, Janet E.

doi:10.1136/gut.4.3.236

Cited by 13 publications

(4 citation statements)

References 13 publications

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“…Several collections of gastric juice were made by using the technique of intragastric neutralization, in which sodium bicarbonate solution (600 mmol/l) was instilled through the nasogastric tube in sufficient volume to maintain the pH of gastric aspirate between 7 and 8. By this means, acid-mediated autodigestion of the proteins in gastric secretion was prevented (Piper, Stiel & Builder, 1963). The samples were freeze-dried, stored at -15°C and reconstituted with deionized water as required.…”

Section: Collection Of Gastrointestinal Secretionsmentioning

confidence: 99%

Studies on the Nature of Complexes Formed by Copper with Human Alimentary Secretions and Their Influence on Copper Absorption in the Rat

Gollan

1975

Clinical Science

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1. Human gastrointestinal secretions formed soluble copper complexes when labelled in vitro with 64Cu. 2. Copper-binding substances of low molecular weight were demonstrated in the saliva, gastric juice and secretin-stimulated duodenal aspirate of nomal subjects by dialysis and gel-chromatography studies. 3. The nature of the copper complexes formed by secretions obtained from patients with Wilson's disease was similar to that oc complexes formed by secretions of normal subjects. 4. Bile contained a copper-binding fraction of high molecular weight which was more concentrated in gall-bladder than hepatic bile. Between pH 5 and pH 8, this component had a greater binding affinity EDTA at a concentration of 10 mmol/1. 5. Absorption of 64Cu from 64Cu-labelled saliva, gastric juice or L-histidine solution (100 mmol/1) administered intraduodenally into groups of rats was similar to that observed in a control series given [Cu]cupric acetate in sodium chloride solution. In contrast, the absorption of 64Cu from labelled hepatic and gall-bladder bile was significantly reduced. 6. The results suggest that dietary copper forms soluble complexes with the alimentary secretions and that these complexes influence absorption of the metal according to their molecular size. The net uptake of ingested copper from the gut lumen ms, low-molecular-weight ligands in the alimentary secretions and a macromolecular copper-binding complex of bile.

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Section: Collection Of Gastrointestinal Secretionsmentioning

confidence: 99%

Studies on the Nature of Complexes Formed by Copper with Human Alimentary Secretions and Their Influence on Copper Absorption in the Rat

Gollan

1975

Clinical Science

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“…Electrophoresis was performed for 2 h at 4 °C and 30 V/cm in 0.06 M sodium borate buffer, pH 9.0 Resolution of protein bands with mucosal preparations was similar to that obtained previously in these laboratories with gastric juice samples (22). It was not improved by reducing the amount of material applied to the strips.…”

Section: Electrophoretic Separation O F Dmct-labelled Componentsmentioning

confidence: 89%

Demethylchlortetracycline-Binding Proteins in Uninvolved Gastric Mucosa of Gastric Carcinoma and Gastric Ulcer Patients

Lo¹,

Thornton²,

Orwell³

et al. 1976

Digestion

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The uninvolved gastric mucosa of gastric ulcer and gastric carcinoma patients has been compared in in vitro studies as regards their capacity to bind demethylchlortetracycline (DMCT). Dialysis experiments demonstrated excessive binding of DMCT in gastric cancer. Several electrophoretic fractions were observed that bound DMCT; it was demonstrated that these fractions differed in the uninvolved mucosa of gastric ulcer and gastric cancer patients.

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“…Moreover, proteins secreted by tumor cells will be at much higher concentration in gastric fluid compared to systemic body fluids (e.g., blood). The diagnostic potential of gastric fluid was recognized in the early 1900s when alterations in its composition and pH were linked to clinical syndromes. − This led to intense efforts to study the protein constituents in gastric fluid and the relationship between their levels and stomach diseases. − However, only a few gastric fluid proteins were identified owing to the limited analytical methods available at the time.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Human Gastric Fluid Proteome Reveals Distinct pH-Dependent Protein Profiles: Implications for Biomarker Studies

Kam

Hennessy²,

Chua

et al. 2011

J. Proteome Res.

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Gastric fluid is a source of gastric cancer biomarkers. However, very little is known about the normal gastric fluid proteome and its biological variations. In this study, we performed a comprehensive analysis of the human gastric fluid proteome using samples obtained from individuals with benign gastric conditions. Gastric fluid proteins were prefractionated using ultracentrifuge filters (3 kDa cutoff) and analyzed by two-dimensional gel electrophoresis (2-DE) and multidimensional LC-MS/MS. Our 2-DE analysis of 170 gastric fluid samples revealed distinct protein profiles for acidic and neutral samples, highlighting pH effects on protein composition. By 2D LC-MS/MS analysis of pooled samples, we identified 284 and 347 proteins in acidic and neutral samples respectively (FDR ≤1%), of which 265 proteins (72.4%) overlapped. However, unlike neutral samples, most proteins in acidic samples were identified from peptides in the filtrate (i.e., <3 kDa). Consistent with this finding, immunoblot analysis of six potential gastric cancer biomarkers rarely detected full-length proteins in acidic samples. These findings have important implications for biomarker studies because a majority of gastric cancer patients have neutral gastric fluid compared to noncancer controls. Consequently, sample stratification, choice of proteomic approaches, and validation strategy can profoundly affect the interpretation of biomarker findings. These observations should help to refine gastric fluid biomarker studies.

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The electrophoretic pattern of normal human gastric juice and of the gastric juice of patients with gastric ulcer and gastric cancer

Cited by 13 publications

References 13 publications

Studies on the Nature of Complexes Formed by Copper with Human Alimentary Secretions and Their Influence on Copper Absorption in the Rat

Studies on the Nature of Complexes Formed by Copper with Human Alimentary Secretions and Their Influence on Copper Absorption in the Rat

Demethylchlortetracycline-Binding Proteins in Uninvolved Gastric Mucosa of Gastric Carcinoma and Gastric Ulcer Patients

Characterization of the Human Gastric Fluid Proteome Reveals Distinct pH-Dependent Protein Profiles: Implications for Biomarker Studies

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