The embryonic stress response to in vitro culture: insight from genomic analysis

Cagnone, Gaël; Sirard, Marc‐André

doi:10.1530/rep-16-0391

Cited by 57 publications

(49 citation statements)

References 200 publications

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“…Despite the increase in BID levels, the expression of genes related to stress response GPX4 and HIF1A was increased in embryos derived from treated oocytes. Embryonic stress response is driven by an oxidative reaction triggered by glutathione peroxidase (GPX), in which reduced glutathione (GSH) acts as a hydrogen peroxide scavenger (Cagnone & Sirard, ). In this way, the upregulation of GPX4 demonstrates an embryonic response to oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Differences in embryonic gene expression and quality indicate the benefit of epidermal growth factor receptor inhibitor during prematuration to improve competence in bovine oocytes

Dall’Acqua

Nunes

Silva

et al. 2019

Reprod Domestic Animals

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ContentsThe epidermal growth factor receptor (EGFR) pathway is directly involved in oocyte meiotic resumption induced by a gonadotropic stimulus. Here, we used an EGFR inhibitor (AG1478) to inhibit spontaneous meiosis resumption in bovine oocytes (EGFR− group) during 8 hr prematuration and assessed the competence of such oo- cytes for embryonic development, apoptosis and gene expression in comparison withControl group which was not prematured. Data are presented as mean ± SEM.Blastocysts rate on day 7 (40.81%, averaged) and hatching rate on day 9 (77.35%, averaged) were unaffected by treatment (p > 0.05). Similarly, treatment did not affect (p > 0.05) the total cell number on day 7 (119.05, averaged) and on day 9 (189.5, averaged). Apoptosis was reduced (p < 0.05) in EGFR− group day 7-embryos compared to Control group (3.7% ± 1.0 vs. 5.2% ± 0.8). Abundance of several transcripts was upregulated (p < 0.05) in EGFR− group, including genes related to embryo development and quality (NANOG and RPLP0), epigenetic regulation (H2AFZ), apoptosis (BID) and stress response (GPX4 and HIF1A). Taken together, the results presented here demonstrated a reduction in the apoptosis index and upregulation of NANOG, H2AFZ and RPLP0 mRNA levels, which are related to embryonic development. Our data suggest that temporary meiosis blockage with EGFR inhibitor during prematuration culture of bovine oocytes may be an interesting strategy to improve embryo quality. K E Y W O R D S apoptosis, embryo development, in vitro maturation | 667 CHEDIEK DALL'ACQUA Et AL.

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Section: Discussionmentioning

confidence: 99%

Differences in embryonic gene expression and quality indicate the benefit of epidermal growth factor receptor inhibitor during prematuration to improve competence in bovine oocytes

Dall’Acqua

Nunes

Silva

et al. 2019

Reprod Domestic Animals

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“…However, multiple other factors such as temperature, osmolality, pH, and embryo density during culture are also known to affect embryo development, and these too may have epigenetic consequences. 19,94 Additionally, new technologies are constantly being developed to further improve IVF success rates. For example, time-lapse microscopy during embryo culture allows for minimal disruption during embryo culture, and is currently being studied to improve live birth rates.…”

Section: Discussionmentioning

confidence: 99%

Embryo Culture Conditions and the Epigenome

Mani

Mainigi

2018

Semin Reprod Med

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Assisted reproductive technologies (ARTs) lead to an increased risk for pregnancy complications, congenital abnormalities, and specific imprinting disorders. Epigenetic dysfunction is thought to be one common mechanism which may be affecting these outcomes. The timing of multiple ART interventions overlaps with developmental time periods that are particularly vulnerable to epigenetic change. In vitro embryo culture is known to impact blastocyst development, in vitro fertilization (IVF) success rates, as well as neonatal outcomes. Embryo culture, in contrast to other procedures involved in ART, is obligatory, and has the highest potential for causing alterations in epigenetic reprograming. In this review, we summarize progress that has been made in exploring the effects of embryo culture, culture media, and oxygen tension on epigenetic regulation in the developing embryo. In humans, it is difficult to isolate the role of embryo culture on epigenetic perturbations. Therefore, additional well-controlled animal studies isolating individual exposures are necessary to minimize the epigenetic effects of modifiable factors utilized during ART. Findings from these studies will likely not only improve IVF success rates but also reduce the risk of adverse perinatal outcomes.

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“…Припускають, що отриманий ембріонами стрес змінює метаболічні шляхи, зокрема надовго пригнічує окислювальне фосфорилювання та активує гліколіз. А тому ставиться завдання покращити умови культивування [3]. Мета роботи -висвітлити нові дані щодо дозрівання ооцитів та отримання ембріонів in vitro.…”

Section: ключові слова: ооцит ембріон In Vitro культивуванняunclassified

New data concerning oocyte maturationand embryo production in vitro

Sarafinyuk¹

2018

Rep. of Vinnytsia Nation. Med. Univ.

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It is reviewed last science data concerning oocyte maturation and pig embryo production in vitro. The aim of the work is to present new data concerning oocyte maturation and embryo production in vitro. Efficiency of different assistant reproductive methods can be improved by treatment of gametes and embryos with sublethal hydrostatic pressure. COCs denudation is better to perform 44 hours from the initiation of maturation procedure. Last 11 hours of maturation is better to perform in medium with decreased quantity of NaCl. Lipid content in oocyte significantly correlates with number of granulose cells per follicle. Larger size pre- and post pubertal oocytes are characterized by greater ability to development. Supplementation of culture medium with glycine leads to increase number of blastocyst cells. Addition of fibroblast growth factor to COCs culture medium benefits its development and blastocyst formation. COCs maturation normalization decreases polyspermy. Bringing endothelial growth factor in COCs medium culture increases rate of blastocyst formation. Different cells processes require different quantity of calcium ion oscillations. Temperature of liquid of preovulatory follicle can be indicator for oocyte quality. 14-days culture of COCs, recovered from early antral follicles, on polyacrylamide gel, results in achievement of larger size oocytes. It is improved method for optical observation of fertilization. It is worked out morula - blastocyst transportation for long distances. Introduction of Lysophosphatidic acid accelerates development of porcine embryos by activating formation of the blastocoel. Conclusion: last literature data show that row of different means of improvement for oocyte maturation and obtaining embryo in vitro is worked out.

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The embryonic stress response to in vitro culture: insight from genomic analysis

Cited by 57 publications

References 200 publications

Differences in embryonic gene expression and quality indicate the benefit of epidermal growth factor receptor inhibitor during prematuration to improve competence in bovine oocytes

Differences in embryonic gene expression and quality indicate the benefit of epidermal growth factor receptor inhibitor during prematuration to improve competence in bovine oocytes

Embryo Culture Conditions and the Epigenome

New data concerning oocyte maturationand embryo production in vitro

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