The endocrine changes, the timing of ovulation and the efficacy of the Doublesynch protocol in the Murrah buffalo (Bubalus bubalis)

Mirmahmoudi, R.; Prakash, B.S.

doi:10.1016/j.ygcen.2012.03.004

Cited by 21 publications

(21 citation statements)

References 25 publications

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“…Similarly, a very low conception rate of 4.7% has been reported in anovular buffalo during unfavourable breeding season (De Rensis et al., ). But, Mirmahmoudi and Prakash () reported higher pregnancy rate (55%) following Doublesynch protocol in anovular buffaloes. Wide variations in conception rates to GnRH‐based protocol can be attributed to the presence of large DF at the time of GnRH1.…”

Section: Discussionmentioning

confidence: 97%

Ovsynch Plus protocol improves ovarian response in anovular Murrah buffaloes in low‐breeding season

Sharma

Phulia

Adnot

et al. 2017

Reprod Domestic Animals

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The objective of the study was to evaluate the efficacy of ovarian response and pregnancy rate in anovular buffaloes following Ovsynch and Ovsynch Plus protocols. Buffaloes (n = 55) were divided into two groups: Ovsynch group (n = 26): GnRH (10 μg, GnRH1) on Day 0, PGF α (25 mg) on Day 7, GnRH (10 μg, GnRH2) on Day 9; Ovsynch Plus group (n = 29): 500 IU equine chorionic gonadotropin (eCG) 72 hr (day -3) prior to Ovsynch protocol, followed by fixed timed artificial insemination (FTAI) 6 and 24 hr after GnRH2 injection in bot groups. Transrectal ultrasonography was performed daily, that is, from day 0 and -3 in Ovsynch and Ovsynch Plus group, respectively for ovarian response and pregnancy diagnosis at day 30 post-insemination. In Ovsynch Plus group, administration of eCG prior to GnRH1 increased (p < .001) the diameter (mm) of dominant follicle (DF) from 10.15 ± 0.26 to 12.23 ± 0.34 within 72 hr of treatment resulting higher ovulatory response to GnRH1. Ovulation after GnRH1 was higher (p < .01) in Ovsynch Plus group (96.6%) than Ovsynch group (61.5%). However, ovulation rate to GnRH2 was similar (p > .05) between groups (Ovsynch group: 76.9% vs. Ovsynch Plus group: 70.0%). Mean DF diameter (mm) that ovulated to both GnRHs was higher (p < .01) than non-ovulated counterparts in both groups (Ovsynch group: 10.80 ± 0.27 vs. 8.47 ± 0.53; Ovsynch Plus group: 11.99 ± 0.24 vs. 9.5 ± 0.63). Pregnancy was established in buffaloes which responded to both GnRHs, irrespective of groups, being higher (p = .52) in Ovsynch Plus group (34.5%) than Ovsynch group (23.1%), though non-significant. In summary, this study showed that eCG inclusion prior to Ovsynch regimen improves ovulatory response in anovular buffaloes during low-breeding season.

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Section: Discussionmentioning

confidence: 97%

Ovsynch Plus protocol improves ovarian response in anovular Murrah buffaloes in low‐breeding season

Sharma

Phulia

Adnot

et al. 2017

Reprod Domestic Animals

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“…Such treatment results in Ovsynch fertility improvement (Thatcher et al 2002;Peters and Pursley 2003;Marquezini et al 2011;Carvalho et al 2015a;b). Two prostaglandin injections with a 14 days interval 28 days before the onset of Ovsynch resulted in an increased conception rates from 37.3% (Ovsynch) to 49.6% (Mirmahmoudi and Prakash 2012). The Ovsynch effectiveness can also be increased by a preliminary PGF injection and a GnRH injection two days later (Carvalho et al 2015b).…”

Section: Ovsynch Modificationsmentioning

confidence: 99%

Probability of pregnancy and risk factors of the Ovsynch program and its modification in dairy cows – a review

et al. 2018

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The Ovsynch programme is one of the most frequently used procedures in managing dairy cattle reproduction. There are many studies evaluating the effectiveness of the Ovsynch programme. However, few of them assess the impact of factors that can lead to a decrease in the pregnancy rate and thus, reduce the success of the Ovsynch programme. This review aims to examine the impact of both individual and environmental factors on the likelihood of pregnancy. The risk factors described include: the body condition, age, number and stage of lactation, production level, occurrence of diseases and postpartum complications, functional status of the ovaries, oestrous cycle phase, temperature, season and year, and program start-up after calving. Possible modifications of the Ovsynch programme are presented in order to increase its effectiveness and to determine the best time to start it. Additionally, attention was paid to pregnancy losses during the Ovsynch programme which ultimately decrease pregnancy rates. The impact of this many factors on the efficiency of the Ovsynch suggests the need to adjust the synchronization program each time to the individual situation of the herd.

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“…Takie leczenie powoduje poprawę płodności u krów poddanych programowi OvSynch (40,46,57). Podając dwie iniekcje prostaglandyny w odstępie 14-dniowym na 28 dni przed rozpoczęciem protokołu OvSynch notowano wzrost odsetka zacielonych krów z 37,3% -uzyskiwanych po zastosowaniu programu OvSynch, do 49,6% (41). Podanie dwóch iniekcji prostaglandyny oraz rozpoczęcie programu OvSynch 12 dni (PreSynch-12) po drugiej iniekcji PG powodowało zwiększenie odsetka samic owulujących po pierwszej iniekcji GnRH oraz wykazujących ciałko żółte w momencie podania prostaglandyny w programie OvSynch (58).…”

Section: Presynchunclassified

“…Jednocześnie wskaźnik zacieleń wzrósł z 29,8% (OvSynch) do 72,8% (DoubleSynch). DoubleSynch z powodzeniem stosowano u bawołów Murrah z anoestrus oraz wykazujących aktywność jajnikową (41,42). Owulacja występowała u 90% krów -22,2 i 23,2 h po pierwszej i drugiej iniekcji GnRH.…”

Section: Programy Alternatywneunclassified

OvSynch program, its modifications and alternative hormonal programs in cow reproduction

Jaśkowski

Herudzińska

Kulus

et al. 2018

Medycyna Weterynaryjna

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The OvSynch program was formulated over 20 years ago (it consists in administering gonadoliberin on the day of starting the procedure, then injecting prostaglandin 7 days after the GnRH initial injection, and administration of GnRH two days after applying PGF2α. The procedure ends in the insemination of a female 16–20 hours after administering the final dose of the hormone) and it is commonly applied in cow reproduction management protocols. It has evolved into numerous modifications which has boosted its effectiveness. They have consisted in replacing GnRH with hCG or pLH, diversifying the time of the final injection of GnRH, which follows the prostaglandin injection, and postponing insemination after the final GnRH dose, as well as changing the dosage of the aforementioned hormones. To expedite the Ovsynch protocol, so-called PreSynch was used, which involved administering two prostaglandin injections with a 14 day interval before applying the initial GnRH dose in protocol OvSynch. The interval between the second prostaglandin injection in the PreSynch program varied (PreSynch-14, PreSynch-12, PreSynch-11, PreSynch-11 and PreSynch). The pregnancy rate can be also improved by introducing GnRH injection before starting the OvSynch program, administered 7, 6 or 4 days before the initial GnRH injection in OvSynch (G7G, G6G, G4G). Higher pregnancy rates, in comparison to the PreSynch-OvSynch program, can be obtained by applying the DoubleOvsynch protocol. In this protocol two OvSynch programs are administered in a 7-day interval. Lately, alternative programs such as DoubleSynch and EstraDoubleSynch have emerged. The DoubleSynch protocol consists in administering PGF2α in day 0, GnRH on the second day, another PGF application on the 9th day, and the second GnRH dose on the 11th day. Artificial insemination is performed in oriented time (TAI). This solution is used in order to achieve a higher percentage of pregnant cows in comparison to the PreSynch+OvSynch protocol. It also enables synchronisation of heat and ovulation, both after the first and the second hormonal treatment of GnRH. In EstraDoubleSynch protocol the second injection of GnRH at the end of the DoubleSynch program is replaced by estradiol benzoate (BE) administration on the 10th day, in order to acquire a higher level of follicle waves synchronisation and ovulation rate. Those programs applied in cow reproduction management can positively influence fertility and boost cow breeding profitability..

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The endocrine changes, the timing of ovulation and the efficacy of the Doublesynch protocol in the Murrah buffalo (Bubalus bubalis)

Cited by 21 publications

References 25 publications

Ovsynch Plus protocol improves ovarian response in anovular Murrah buffaloes in low‐breeding season

Ovsynch Plus protocol improves ovarian response in anovular Murrah buffaloes in low‐breeding season

Probability of pregnancy and risk factors of the Ovsynch program and its modification in dairy cows – a review

OvSynch program, its modifications and alternative hormonal programs in cow reproduction

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