The epidermal phenotype during initiation of the psoriatic lesion in the symptomless margin of relapsing psoriasis

Castelijns, F.A.C.M.; Gerritsen, Marie‐Jeanne P.; Vlijmen-Willems, Ivonne M.J.J. van; Erp, P.E.J. van; Kerkhof, P.C.M. van de

doi:10.1016/s0190-9622(99)70077-0

Cited by 32 publications

(36 citation statements)

References 40 publications

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“…The immunohistochemical analysis of epidermal markers in transgenic mice observed the expression of the differentiation and proliferation markers in the suprabasal layers, which are normally confined to the basal layer. Castelijans et al 19 had observed similar immunohistochemical results in human psoriatic skin. These histological changes in the skin appear to be caused by circulating IL-20, because even mice expressing transgene in other tissues (liver) were similarly affected.…”

Section: Discussionmentioning

confidence: 62%

Polymorphisms in the interleukin-20 gene: relationships to plaque-type psoriasis

et al. 2004

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We analyzed the frequency of single-nucleotide polymorphisms (SNPs) at positions À1053 (rs 2981572), 1380 (rs 2981573), 1462 (rs 2232360), and 3978 (rs 1518108) of the human interleukin-20 (IL-20) gene by tetraprimer ARMS-PCR method. A significant association between patients with psoriasis and the G allele at position À1053 (Po0.05) was established. The pairwise linkage disequilibrium (LD) matrix showed that the nearly complete LD was present within the polymorphisms at positions À1053, 1380, and 1462 of the IL-20 gene. We found that patients with plaque psoriasis had a higher frequency of the HT3 GAA haplotype (Po0.01, OR 2.341, 95% CI: 1.346-4.074) compared to the control group. Likewise, the HT3 GAA haplotype was associated with an increased risk of early-onset psoriasis (Po0.01, OR 2.305, 95% CI: 1.285-4.132), late onset of disease (Po0.01, OR 2.542, 95% CI: 1.266-5.102), familial psoriasis (Po0.02, OR 2.220, 95% CI: 1.249-3.945), and sporadic disease (Po0.01, OR 2.523, 95% CI: 1.390-4.580). Our data indicate that IL-20 gene polymorphisms should have a role in determining susceptibility to plaque-type psoriasis. The possible role of the studied SNPs in the regulation of the expression of IL-20 is unknown yet and needs further studies.

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Section: Discussionmentioning

confidence: 62%

Polymorphisms in the interleukin-20 gene: relationships to plaque-type psoriasis

et al. 2004

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“…Although activated T lymphocytes and dendritic cells are now generally accepted to be important in the maintenance of psoriasis, both the clinical phenotypes (lesion thickness and scaling) and histological phenotypes are dominated by abnormalities in keratinocyte differentiation and proliferation [19]. To histologically evaluate psoriatic activity, cell proliferation may be evaluated by a proliferating index, such as Ki-67 [20]. Filaggrin is an important protein in the process of differentiation expressed only in well-differentiated keratinized epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

The Efficacy and Safety of Topically Applied Indigo Naturalis Ointment in Patients with Plaque-Type Psoriasis

Lin

Wong²,

Chang³

et al. 2007

Dermatology

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Background: It has been reported in the Chinese literature that indigo naturalis exhibits potential antipsoriatic effects in systemic therapy. Objective: To evaluate the efficacy and safety of topically applied indigo naturalis on treating plaque-type psoriasis and to analyze the histological change in skin tissues. Methods: Fourteen patients with chronic plaque psoriasis were enrolled. The patients were topically applied with either indigo naturalis ointment or vehicle ointment on contralateral skin lesions daily for 8 weeks. Efficacy was evaluated on the basis of the clinical scores, including induration, scaling, erythema and clearing percentage. At the end of treatment, skin punch biopsies were taken and prepared for the immunohistochemical analysis. Results: A significant reduction in clinical scores was achieved with topically applied indigo naturalis ointment. Analysis of biopsies showed a marked improvement of skin histology. The expressions of proliferating marker Ki-67 and inflammatory marker CD3 were decreased, but the differentiation marker such as filaggrin was increased in the epidermis after indigo naturalis ointment treatment. Conclusions: The results suggest that topical application of indigo naturalis ointment may be a novel, safe and effective therapy for psoriasis that is mediated, at least in part, by modulating the proliferation and differentiation of keratinocytes in epidermis, as well as by inhibiting the infiltration of T lymphocytes and therefore the subsequent inflammatory reactions in psoriatic lesions.

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“…So in total 47 specimens were examined. On each psoriatic lesion clinical parameters for erythema, induration and scaling were assessed on a 5-point scale (by the same investigator [16], allowing a maximum score for the severest psoriatic lesion of 12. Serial sections were used to perform the experiments described below.…”

Section: Methodsmentioning

confidence: 99%

“…A high-temperature microwave antigen retrieval technique [7]was needed to reveal the Ki-67 epitope. The MIB-1 antibody (Immunotech, Marseille, France) was used according to a protocol formerly described [16, 17]. …”

Section: Methodsmentioning

confidence: 99%

Cell-Kinetic Evidence for Increased Recruitment of Cycling Epidermal Cells in Psoriasis: The Ratio of Histone and Ki-67 Antigen Expression Is Constant

et al. 2000

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Background: One of the hallmarks of the psoriatic plaque is increased epidermal proliferation. Whether this is the result of an increased recruitment of cycling epidermal cells or a decrease in cell cycle time has been a matter of debate for years. Objective: Calculating cell-kinetic information from the number of S phase cells in psoriasis by in situ hybridisation using a histone probe and the number of cycling epidermal cells by immunohistochemistry using the MIB-1 antibody. Methods: Immunohistochemistry and non-isotopic in situ hybridisation were performed on serial sections of 33 untreated psoriatic samples and 14 tacalcitol-treated samples. Results: The labelling index (number of cells in S phase/number cycling cells per millimetre length of section) in psoriatic untreated as well as in treated plaques is 16%. The amount of S phase cells in our experiment is equal compared with the number of cells in S phase as determined by BrdU incorporation. Conclusion: Using this direct approach to study cell-kinetic behaviour of psoriatic skin, we reconfirm that the psoriatic abnormality is due to a defect in the G₀–G₁ recruitment mechanism (by increased recruitment of G₀ cells), a decrease in apoptosis or an increase in the number of cell divisions in the transit-amplifying compartment, rather than a reduction in the cell cycle time.

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The epidermal phenotype during initiation of the psoriatic lesion in the symptomless margin of relapsing psoriasis

Cited by 32 publications

References 40 publications

Polymorphisms in the interleukin-20 gene: relationships to plaque-type psoriasis

Polymorphisms in the interleukin-20 gene: relationships to plaque-type psoriasis

The Efficacy and Safety of Topically Applied Indigo Naturalis Ointment in Patients with Plaque-Type Psoriasis

Cell-Kinetic Evidence for Increased Recruitment of Cycling Epidermal Cells in Psoriasis: The Ratio of Histone and Ki-67 Antigen Expression Is Constant

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