The equilibrium between carbon monoxide and sheep haemoglobin at very high percentage saturations

Roughton, F. J. W.

doi:10.1113/jphysiol.1954.sp005215

Cited by 56 publications

(35 citation statements)

References 14 publications

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“…There is at present no satisfactory direct method for studying the oxygen equilibrium curve in this saturation range. However, Roughton (1954) had shown that in this saturation range and at pH 9-1 the oxygen and carbon monoxide equilibrium curves are related, and if L4 is known K4 may be found from the relationship L4 = MK4. M is the value of…”

Section: Equilibrium 8tudiesmentioning

confidence: 95%

“…Measurements on the carbon monoxide equilibrium in the range 99 0-99.5% saturation at 190 C were made by the method described by Roughton (1954). From two to four determinations were made for each haemoglobin.…”

Section: Equilibrium 8tudiesmentioning

confidence: 99%

“…Duplicate determinations of M were made on each haemoglobin sample by the method described by Roughton (1954). In using this method it is assumed that carbon monoxide is conserved.…”

Section: Equilibrium 8tudiesmentioning

confidence: 99%

“…Because of this lower accuracy, the mathematical procedure described by Roughton (1954) Roughton (1954).…”

Section: Equilibrium Studie8mentioning

confidence: 99%

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Kinetics of the reactions of two sheep haemoglobins with oxygen and carbon monoxide

Kernohan¹

1961

The Journal of Physiology

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Section: Equilibrium 8tudiesmentioning

confidence: 95%

Section: Equilibrium 8tudiesmentioning

confidence: 99%

“…Duplicate determinations of M were made on each haemoglobin sample by the method described by Roughton (1954). In using this method it is assumed that carbon monoxide is conserved.…”

Section: Equilibrium 8tudiesmentioning

confidence: 99%

“…Because of this lower accuracy, the mathematical procedure described by Roughton (1954) Roughton (1954).…”

Section: Equilibrium Studie8mentioning

confidence: 99%

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Kinetics of the reactions of two sheep haemoglobins with oxygen and carbon monoxide

Kernohan¹

1961

The Journal of Physiology

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“…With haemoglobin solution containing more than 0 5 atm partial pressure of CO, no significant amounts of CO dissociate from chemical combination with the haemoglobin during the 5 min shaking period, but with low partial pressures of CO a correction was necessary for this factor. The recent data of Roughton (1951) on dissociation of carboxyhaemoglobin between 98 and 100% saturation were used for this purpose.…”

Section: Methods I Diffusion Apparatusmentioning

confidence: 99%

The diffusion coefficients of carbon monoxide and nitrogen in haemoglobin solutions

Longmuir¹,

Roughton²

1952

The Journal of Physiology

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Krogh's (1919a) measurements of the rate of diffusion of 02, N2, CO and CO02 through animal tissues have formed the basis of many calculations of the role of diffusion in physiological processes (see, for example, Krogh, 1919b;Hill, 1928;Roughton, 1932;Jacobs, 1935). No information, however, appears to exist as to the rate of diffusion of these dissolved gases through homogeneous solutions of proteins: the value of the diffusion coefficients of 02 and CO in concentrated haemoglobin solutions has, in particular, been a desideratum in the theoretical investigations of Roughton (1932) and Nicolson & Roughton (1951) on the rate of exchange of 02 and CO between the interior of the red blood cells and the surrounding fluid. At the concentrations found in the red cells, the haemoglobin molecules must, according to Perutz (1948), be practically touching and there should accordingly be both marked geometrical obstruction and viscous resistance to the passage of small molecules of dissolved gases between the large protein molecules. The relation between the value of the diffusion coefficients and the protein concentration is therefore also of some theoretical interest.In the present paper we have determined the diffusion coefficients of N2 and CO in haemoglobin solutions ranging from 0 to 45 g haemoglobin/100 ml., the latter concentration being appreciably higher than that usually found in the red cells. In the case of N2 there has been no complication from any simultaneous chemical reactions of the penetrating substance: with CO, on the other hand, there has, from time to time, been trouble from side reactions even when the concentrations of CO have been such as to ensure that the haemoglobin is at least 99.99 % saturated with CO. Fortunately for our present purpose, N2 and CO have the same molecular weight and are very similar in many of their physical properties, including solubility. A priori, their diffusion coefficients in haemoglobin solutions should be about the same: molecules has also to be taken into account. Perutz's work would appear to make the need for such an allowance very unlikely, but fortunately we have found it possible, by means of an alternative method to our main one of measuring the diffusion coefficients of CO in haemoglobin solutions, to obtain direct experimental evidence on this point. METHODS I. Diffusion apparatusIn most of the experiments diffusion was measured between a gas phase containing CO or N2 at 1 atm pressure, and haemoglobin solution containing these gases in solution, but at different partial pressures from those in the gas phase. The apparatus, which was specially designed for use in conjunction with the Van Slyke gasometric methods, is shown in Fig. 1. It consists essentially of a large glass tap, T (bore about 1 cm), one lead of which is closed at the end whilst the other is fused to a straight capillary bore tap, U, and also to a 3-way capillary bore tap, V, the 265 266 I. S. LONGMUIR AND F. J. W. ROUGHTON leads from which are inclined at angles of 1200 to each other. On the...

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Examination of Haldane's first law for the partition of CO and O₂ to hemoglobin A₀

Wyman¹,

Bishop²,

Richey³

et al. 1982

Biopolymers

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SynopsisA study of the oxygen replacement reaction of carbon monoxide-saturated hemoglobin ( H b b ) was carried out using spectroscopic, calorimetric, and pH titration methods. Under fully saturated conditions the replacement reaction can be defined by a single partition constant over all ratios of bound oxygen to carbon monoxide. This indicates that under saturating conditions Haldane's fist law for the liiand binding of gas mixtures holds for any CO/Oz ratio.It further shows that there is no appreciable difference in relative CO-02 affinity between the a-and @-chains. The same partition coefficient was found to hold for different pH, buffer, and allosteric effector conditions. The lack of any pH dependence of the partition coefficient was confirmed by the absence of proton changes for the replacement reaction. The temperature dependence of the partition coefficient and calorimetric results yield a value for the enthalpy of the reaction of -3.65 f 0.29 kcal/mol/heme.

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The equilibrium between carbon monoxide and sheep haemoglobin at very high percentage saturations

Abstract: According to the intermediate compound hypothesis (Adair, 1925)

Cited by 56 publications

References 14 publications

Kinetics of the reactions of two sheep haemoglobins with oxygen and carbon monoxide

Kinetics of the reactions of two sheep haemoglobins with oxygen and carbon monoxide

The diffusion coefficients of carbon monoxide and nitrogen in haemoglobin solutions

Examination of Haldane's first law for the partition of CO and O₂ to hemoglobin A₀

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The equilibrium between carbon monoxide and sheep haemoglobin at very high percentage saturations

Abstract: According to the intermediate compound hypothesis (Adair, 1925)

Cited by 56 publications

References 14 publications

Kinetics of the reactions of two sheep haemoglobins with oxygen and carbon monoxide

Kinetics of the reactions of two sheep haemoglobins with oxygen and carbon monoxide

The diffusion coefficients of carbon monoxide and nitrogen in haemoglobin solutions

Examination of Haldane's first law for the partition of CO and O2 to hemoglobin A0

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Examination of Haldane's first law for the partition of CO and O₂ to hemoglobin A₀