The essential anti-angiogenic strategies in cartilage engineering and osteoarthritic cartilage repair

Pei, Yixuan Amy; Song, Chen; Pei, Ming

doi:10.1007/s00018-021-04105-0

Cited by 22 publications

(12 citation statements)

References 266 publications

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“…The quantitative analysis of vascular endothelial growth factor A (VEGFA) expression, a vascularization marker, was upregulated in the HAMC group, detected by quantitative polymerase chain reaction (qPCR) and western blotting (Figure 6g,h). [ 61 ] Consistently, the CD31 was also upregulated in HAMC group compared with LHAMC group, indicating the angiogenesis occurs (Figure S33, Supporting Information). It is worth noting that neovascularization extending from the subchondral bone to the avascular articular cartilage can lead to articular cartilage ossification and abnormal function.…”

Section: Resultsmentioning

confidence: 99%

Cartilage Lacuna‐Inspired Microcarriers Drive Hyaline Neocartilage Regeneration

et al. 2023

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Cartilage equivalents from hydrogels containing chondrocytes exhibit excellent potential in hyaline cartilage regeneration, yet current approaches have limited success at reconstituting the architecture to culture nondifferentiated chondrocytes in vitro. In this study, specially designed lacunar hyaluronic acid microcarriers (LHAMCs) with mechanotransductive conditions that rapidly form stable hyaluronic acid (HA) N‐hydroxy succinimide ester (NHS‐ester) are reported. Specifically, carboxyl‐functionalized HA is linked to collagen type I via amide‐crosslinking, and gas foaming produced by ammonium bicarbonate forms concave surface of the microcarriers. The temporal 3D culture of chondrocytes on LHAMCs uniquely remodels the extracellular matrix to induce hyaline cartilaginous microtissue regeneration and prevents an anaerobic‐to‐aerobic metabolism transition in response to the geometric constraints. Furthermore, by inhibiting the canonical Wnt pathway, LHAMCs prevent β‐catenin translocation to the nucleus, repressing chondrocyte dedifferentiation. Additionally, the subcutaneous implantation model indicates that LHAMCs display favorable cytocompatibility and drive robust hyaline chondrocyte‐derived neocartilage formation. These findings reveal a novel strategy for regulating chondrocyte dedifferentiation. The current study paves the way for a better understanding of geometrical insight clues into mechanotransduction interaction in regulating cell fate, opening new avenues for advancing tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Cartilage Lacuna‐Inspired Microcarriers Drive Hyaline Neocartilage Regeneration

et al. 2023

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“…We found that cell-implanted groups had strong GFP signals limited to the interface between newly formed cartilage and subchondral bone. Given that cartilage hypertrophy adjacent to the defects is expected due to blood vessel invasion into the lesion site [ 36 ], it is not surprising to observe that these areas were positively stained for type X collagen. We also found these areas were positive for types I and II collagen and negative for GAG, indicative of fibrocartilage.…”

Section: Discussionmentioning

confidence: 99%

Matrix from urine stem cells boosts tissue-specific stem cell mediated functional cartilage reconstruction

Pei

Zhou

et al. 2023

Bioactive Materials

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“…In addition, there are many endogenous anti‐angiogenic factors, such as endostatin, which can competitively bind to VEGFR‐2 on the surface of endothelial cells and inhibit VEGF‐induced proliferation and migration of endothelial cells; angiostatin, which can inhibit MMP‐mediated migration of endothelial cells; and troponin I, which can interact with FGF‐2 receptor on the surface of endothelial cells and inhibit the proliferation of both activated and non‐activated endothelial cells 151–154 . Some of these factors have been applied in cartilage repair and showed good reparative effects.…”

Section: Anti‐angiogenesis Strategies In Tissue Engineeringmentioning

confidence: 99%

Mechanisms of vascular invasion after cartilage injury and potential engineering cartilage treatment strategies

Zhao,

Sun,

et al. 2024

The FASEB Journal

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Articular cartilage injury is one of the most common diseases in orthopedic clinics. Following an articular cartilage injury, an inability to resist vascular invasion can result in cartilage calcification by newly formed blood vessels. This process ultimately leads to the loss of joint function, significantly impacting the patient's quality of life. As a result, developing anti‐angiogenic methods to repair damaged cartilage has become a popular research topic. Despite this, tissue engineering, as an anti‐angiogenic strategy in cartilage injury repair, has not yet been adequately investigated. This exhaustive literature review mainly focused on the process and mechanism of vascular invasion in articular cartilage injury repair and summarized the major regulatory factors and signaling pathways affecting angiogenesis in the process of cartilage injury. We aimed to discuss several potential methods for engineering cartilage repair with anti‐angiogenic strategies. Three anti‐angiogenic tissue engineering methods were identified, including administering angiogenesis inhibitors, applying scaffolds to manage angiogenesis, and utilizing in vitro bioreactors to enhance the therapeutic properties of cultured chondrocytes. The advantages and disadvantages of each strategy were also analyzed. By exploring these anti‐angiogenic tissue engineering methods, we hope to provide guidance for researchers in related fields for future research and development in cartilage repair.

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The essential anti-angiogenic strategies in cartilage engineering and osteoarthritic cartilage repair

Cited by 22 publications

References 266 publications

Cartilage Lacuna‐Inspired Microcarriers Drive Hyaline Neocartilage Regeneration

Cartilage Lacuna‐Inspired Microcarriers Drive Hyaline Neocartilage Regeneration

Matrix from urine stem cells boosts tissue-specific stem cell mediated functional cartilage reconstruction

Mechanisms of vascular invasion after cartilage injury and potential engineering cartilage treatment strategies

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