The Establishment of Some Microflora Associated Biochemical Characteristics in Feces from Children during the First Years of Life

Norin, K. E.; Gustafsson, Bengt E.; Lindblad, B. S.; Midtvedt, Tore

doi:10.1111/j.1651-2227.1985.tb10951.x

Cited by 80 publications

(38 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The establishment of a mucindegrading flora in the human colon is a gradual process that begins in some infants as early as 3 mo of age (17,18). In the study of structures as complex as mucins, it may be difficult to determine whether changes in composition are the result of differences in their synthesis or bacterial degradation or of the analytical techniques used.…”

Section: Discussionmentioning

confidence: 99%

Age and Diet Affect the Composition of Porcine Colonic Mucins

1993

View full text Add to dashboard Cite

ABSTRACT. Colonic mucins may serve a s a defense mechanism by binding bacterial, viral, or dietary lectins, thereby preventing them from attaching to the intestinal epithelium. Presumably, the composition of the mucins would be responsible for this phenomenon, and the composition of mucins from mature mammals would be the most effective in binding lectins. To determine whether differences in diet and/or age affect the composition of colonic mucins, we scraped fresh colonic mucosae from pigs a t 0 ( n = 3), 7 ( n = 3), 21 ( n = 3), and 180 (n = 3) d of age and purified the mucins from these mucosal scrapings. Mucins were purified by ribonuclease and deoxyribonuclease digestion, high-performance size-exclusion chromatography, and cesium chloride density-gradient ultracentrifugation. The 180-d-old pig was considered mature. No changes were observed in any of the variables analyzed in the 7-d-old animals. No changes were observed in quantities of galactosamine and galactose. The amounts of fucose and glucosamine increased by 165 and 37%, respectively, ( p < 0.05) from d 0 to d 21 in the sow-fed animals, at which time fucose and glucosamine content were 48 and 22% greater, respectively, than in the 21-d-old, artificially fed group ( p < 0.05). A further significant increase in fucose content was observed in the mucins from mature animals. The sulfate content in the 21-d-old, sow-fed animals was significantly lower than in both the newborn and the 21-d-old artificially fed animals. The sulfate content in all three of these groups, however, was significantly higher than that observed in the mucins of mature animals. At 21 d of age, the protein content was significantly lower than a t birth for both diet groups but significantly higher than in the mucins of mature pigs. The evidence indicates that the composition of colonic mucins in 21-d-old sow-fed pigs was more mature than that in the artificially fed counterparts, with respect to fucose, glucosamine, and sulfate. Therefore, if a mature mucin composition is, in fact, a more effective defense against intestinal infection, young breast-fed animals may have an advantage over young artificially fed animals. (Pediatr Res 33: 564-567, 1993)

show abstract

Section: Discussionmentioning

confidence: 99%

Age and Diet Affect the Composition of Porcine Colonic Mucins

1993

View full text Add to dashboard Cite

show abstract

“…Postnatal development of the GIT bacterial assemblages is complex [32,33] , involves changes in the absolute and relative abundances of species [34][35][36][37] and metabolic processes [38] . Notable are the changes in the abundances of naturally occurring species of lactobacilli in the intestines of pigs [39] and human infants [34] , and for probiotic bacteria introduced into the neonatal GIT [37 , present study].…”

Section: Discussionmentioning

confidence: 99%

Maternal-to-Infant Transmission of Probiotics: Concept Validation in Mice, Rats, and Pigs

Buddington¹,

Williams²,

Kostek³

et al. 2009

Neonatology

View full text Add to dashboard Cite

Background: Postnatal introduction of probiotics results in a low incidence of colonization, whereas maternal fecal and vaginal bacteria colonize the gastrointestinal tract (GIT) of vaginally delivered infants. Objective: We tested if probiotic bacteria, fed to three pregnant animal models, would colonize the GIT of offspring delivered vaginally. Methods: Probiotic strains of Lactobacillus acidophilus and Bifidobacterium lactis were fed to pregnant mice, rats, and sows for at least 7 days prior to vaginal delivery. Cultural approaches and genotyping were used to determine if the probiotic bacteria colonized the GIT after birth. Results: The probiotic bacteria were detected in the feces and vagina of maternal mice, rats, and sows after, but not before, administration. L. acidophilus was detected at postnatal day 14 in 22, 33, and 75% of the mice, rats, and pigs, respectively, and after weaning in 35% of the mice and 1 of 5 pigs. B. lactis was present at postnatal day 14 in 30 and 80% of the mice and pigs. Bacterial assemblages in the GIT of the colonized young differed from those in which the probiotics were not detected. Conclusions: Probiotic bacteria administered to mothers during late gestation are transferred to infants born vaginally and influence the assemblages of GIT bacteria. However, colonization of the neonatal GIT and persistence past weaning does not occur in all offspring and varies among probiotics and animal models.

show abstract

“…Mucin degradation has been recognized as a normal process of mucus turn-over in the GI tract, starting a few months after birth. 78 Midtvedt et al studied the establishment of the mucin-degrading microbiota from 30 Swedish children from birth to the age of two years old, by analyzing their fecal samples. They found that the establishment of mucin-degrading bacteria starts during the first months of life, and is completed when the children are around two years old.…”

Section: Mucin Binding By Bacteriamentioning

confidence: 99%