The Estimation of Nucleic Acids in Some Algae and Higher Plants

Smillie, Robert M.; Krotkov, G.

doi:10.1139/b60-004

Cited by 262 publications

(75 citation statements)

References 22 publications

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“…RNA was prepared using Method B of Loening and Ingle (8) and purified by the method of Ralph and Bellamy (17). Total RNA and DNA were estimated by the method of Smillie and Krotkov (21).…”

Section: Methodsmentioning

confidence: 99%

Developmental Changes in Ribosomal Ribonucleic Acid and Fraction I Protein in Wheat Leaves

Patterson

Smillie

1971

Plant Physiol.

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In light-grown wheat (Triticumn aestivum L.) seedlings, the amount of chloroplast and cytoplasmic ribosomal RNA increased to a maximum in the first leaf near the end of its growth and declined by about 60% in the following 3 days. While total ribosomal RNA was declining, labeled uracil was still incorporated into cytoplasmic ribosomal RNA, but the rate of incorporation into chloroplast ribosomal RNA fell by more than 80%, as did the incorporation of labeled leucine into fraction I protein. Either there is greater replacement of cytoplasmic ribosomal RNA than chloroplast ribosomal RNA in mature leaves, or chloroplasts are able to repress the incorporation of exogenous precursor when there is no net synthesis of RNA.In leaves there are two major systems of protein synthesis (2, 18), one using the ribosomes of the cytoplasm, and the other using the smaller ribosomes of the chloroplast (4,19,20). Although it is difficult to estimate relative amounts of the two classes of ribosomes directly, they each contain characteristic species of RNA which can be separated and estimated using polyacrylamide gel electrophoresis (6,8). The larger subunit of chloroplast rRNA in particular is somewhat unstable during isolation (6). Therefore, in order to study the net changes in rRNA at different stages of the development of wheat leaves, we have measured the amounts of the smaller subunits of chloroplast and cytoplasmic rRNA. We have compared the changes in rRNA levels with changes in the level of fraction I protein, which is probably made on the chloroplast ribosomes (20). In addition, the ability of wheat leaves to incorporate exogenous precursor into chloroplast rRNA, cytoplasmic rRNA, and fraction I protein has been measured at different stages of development. MATERIALS AND METHODSGrowth of Wheat. Wheat (Triticum aestivum L., var. Timgalen) was grown in vermiculite with a 24 hr cycle of 12-hr light and dark, at 22 C and 16 C, respectively. Light (about 9,000 lux) was supplied by equal numbers of Philips MCFER warm white and Sylvania Grolux fluorescent tubes. Sowing and harvesting was midway through the light period, and the nutrient solution used was that of Hoagland and Arnon (5).Incorporation of Radioisotopes. Leaves were dissected from the coleoptile and cut at the ligule, surface sterilized with 0.1% cetyltrimethylammonium bromide, thoroughly rinsed with sterile distilled water, and cut into 3-mm sections. Tissue (0.5 g) was incubated with 1 ml of precursor solution for 6 hr with intermittent shaking in light at 22 C. 5-3H-Uracil (240 ,uc/ml; 1 c/mmole) was used as a precursor of RNA, and 'H-leucine, generally labeled (100 ,uc/ml; 50 mc/mmole), was used as a precursor of protein. Incubation was begun 3 hr before the middle of the light period.Extraction of RNA. RNA was prepared using Method B of Loening and Ingle (8) and purified by the method of Ralph and Bellamy (17). Total RNA and DNA were estimated by the method of Smillie and Krotkov (21).Extraction of Fraction I Protein. Leaves were frozen in liquid nitrogen a...

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“…RNA was prepared using Method B of Loening and Ingle (8) and purified by the method of Ralph and Bellamy (17). Total RNA and DNA were estimated by the method of Smillie and Krotkov (21).…”

Section: Methodsmentioning

confidence: 99%

Developmental Changes in Ribosomal Ribonucleic Acid and Fraction I Protein in Wheat Leaves

Patterson

Smillie

1971

Plant Physiol.

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“…1 column as described by SMILLIE and KROTKOV (1960). No significant differences were found in absorption values at 665 m p obtained by the different procedures.…”

Section: E Determination Of Number Of Cells Per Leafmentioning

confidence: 82%

“…1 column as described by SMILLIE and KROTKOV (1960). This purification did not result in significant differences in absorption values at 600 m,u.…”

Section: E Determination Of Number Of Cells Per Leafmentioning

confidence: 94%

Macromolecular Physiology of Plastids V

Gyldenholm¹

2009

Hereditas

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“…Nas mesmas épocas, em folhas apicais fisiologicamente maduras foram determinadas as frações de P, conforme método descrito por Smille & Krotkov (1960) e Hogue et al (1970) modificado por Martinez (1992) e Furtini Neto et al (1998), e a atividade in vitro da fosfatase ácida, segundo método proposto por Santos (1985).…”

Section: Methodsunclassified

Interações silício-fósforo em solos cultivados com eucalipto em casa de vegetação

Carvalho

Neto

Santos

et al. 2001

Pesq. agropec. bras.

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Este trabalho teve o objetivo de avaliar as interações Si-P em dois solos, por meio do estado nutricional de P em mudas de Eucalyptus grandis sob efeito de aplicação de Si. Foram conduzidos dois experimentos em casa de vegetação, utilizando como substratos as camadas superficiais (0-20 cm de profundidade) de um Latossolo Vermelho-Escuro fase cerrado e de um Cambissolo fase campo cerrado da zona fisiográfica Campos das Vertentes, MG . Cada solo foi submetido a três incubações seqüenciais: CaCO3 + MgCO3 para manter o pH em torno de 6, fertilização básica, incluindo o P em dose única, para manter 0,2 mg L-1 de P em solução e seis doses de Si (CaSiO3) definidas com base na dose de P. Foram avaliados a produção de matéria seca da parte aérea (MSPA), conteúdo de P e Si na MSPA e frações fosfatadas e fosfatase ácida em folhas apicais aos 60, 90 e 120 dias após o transplantio das mudas para os vasos. Ajustaram-se superfícies de resposta para essas variáveis em razão das doses de Si e das épocas. Mudas cultivadas no Cambissolo tiveram ganho de 15,25% no conteúdo de P na MSPA. Frações fosfatadas e atividade da fosfatase ácida em plantas não mostraram sensibilidade para avaliar a interação Si-P nos dois solos.

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The Estimation of Nucleic Acids in Some Algae and Higher Plants

Cited by 262 publications

References 22 publications

Developmental Changes in Ribosomal Ribonucleic Acid and Fraction I Protein in Wheat Leaves

Developmental Changes in Ribosomal Ribonucleic Acid and Fraction I Protein in Wheat Leaves

Macromolecular Physiology of Plastids V

Interações silício-fósforo em solos cultivados com eucalipto em casa de vegetação

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