The ether lipid-deficient mouse: Tracking down plasmalogen functions

Gorgas, Karin; Teigler, Andre; Komljenovic, Dorde; Just, Wilhelm W.

doi:10.1016/j.bbamcr.2006.08.038

Cited by 203 publications

(199 citation statements)

References 167 publications

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“…These lipids are important for cell physiology, although their biological functions on the molecular levels are poorly understood. For example, in mice ether lipid deficiency leads to male infertility, defects in eye development, and abnormalities in the central nervous system (20). In addition, altered levels of ether lipids have been noted in several genetic disorders and pathologies such as Zellweger syndrome, Alzheimer disease, and diabetes (19 -21).…”

mentioning

confidence: 99%

The Ether Lipid Precursor Hexadecylglycerol Stimulates the Release and Changes the Composition of Exosomes Derived from PC-3 Cells

Phuyal

Skotland

Hessvik

et al. 2015

Journal of Biological Chemistry

119

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Background: Lipids are emerging as important constituents of the molecular machinery involved in the formation and release of exosomes. Results: The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes. Conclusion: Ether lipids are important modulators of exosome release. Significance: By knowing the machinery involved in exosome release, it would be possible to modulate this process.

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confidence: 99%

The Ether Lipid Precursor Hexadecylglycerol Stimulates the Release and Changes the Composition of Exosomes Derived from PC-3 Cells

Phuyal

Skotland

Hessvik

et al. 2015

Journal of Biological Chemistry

119

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“…alkylglycerols | lipidomics | macrophages | RAW264.7 | tetrahydrobiopterin A lkylglycerols are a subclass of the vast family of ether lipids that are essential membrane constituents (e.g., in neurons), are important for spermatogenesis, and protect the eye from cataract (1). Furthermore, they are also involved in many signaling pathways (2).…”

mentioning

confidence: 99%

Tetrahydrobiopterin and alkylglycerol monooxygenase substantially alter the murine macrophage lipidome

Watschinger

Keller

McNeill

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Tetrahydrobiopterin is a cofactor synthesized from GTP with well-known roles in enzymatic nitric oxide synthesis and aromatic amino acid hydroxylation. It is used to treat mild forms of phenylketonuria. Less is known about the role of tetrahydrobiopterin in lipid metabolism, although it is essential for irreversible ether lipid cleavage by alkylglycerol monooxygenase. Here we found intracellular alkylglycerol monooxygenase activity to be an important regulator of alkylglycerol metabolism in intact murine RAW264.7 macrophage-like cells. Alkylglycerol monooxygenase was expressed and active also in primary mouse bone marrowderived monocytes and "alternatively activated" M2 macrophages obtained by interleukin 4 treatment, but almost missing in M1 macrophages obtained by IFN-γ and lipopolysaccharide treatment. The cellular lipidome of RAW264.7 was markedly changed in a parallel way by modulation of alkylglycerol monooxygenase expression and of tetrahydrobiopterin biosynthesis affecting not only various ether lipid species upstream of alkylglycerol monooxygenase but also other more complex lipids including glycosylated ceramides and cardiolipins, which have no direct connection to ether lipid pathways. Alkylglycerol monooxygenase activity manipulation modulated the IFN-γ/lipopolysaccharide-induced expression of inducible nitric oxide synthase, interleukin-1β, and interleukin 1 receptor antagonist but not transforming growth factor β1, suggesting that alkylglycerol monooxygenase activity affects IFN-γ/lipopolysaccharide signaling. Our results demonstrate a central role of tetrahydrobiopterin and alkylglycerol monooxygenase in ether lipid metabolism of murine macrophages and reveal that alteration of alkylglycerol monooxygenase activity has a profound impact on the lipidome also beyond the class of ether lipids. alkylglycerols | lipidomics | macrophages | RAW264.7 | tetrahydrobiopterin

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“…Spermatozoa are unique among mammalian cells in that they contain high levels of ether-linked phospholipids (plasmalogens) [21]. A previous study [22] revealed that plasmalogens represented on average ~25% of the phospholipids of the membranes of stallion spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

Fatty acids and plasmalogens of the phospholipids of the sperm membranes and their relation with the post-thaw quality of stallion spermatozoa

et al. 2011

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Fatty acids and plasmalogens of the phospholipids of the sperm membranes and their relation with the post-thaw quality of stallion spermatozoa, 2011, THERIOGENOLOGY, (75) AbstractFatty acids and plasmalogens were extracted from the phospholipids of the plasma membrane of stallion spermatozoa, to determine their relation with sperm quality after freezing and thawing. Sperm quality was rated using a quality index that combined the results of the analysis of sperm motility and velocity (CASA analysis), membrane status and mitochondrial membrane potential (flow cytometry) post thaw. Receiving operating system (ROC) curves were used to evaluate the value of specific lipid components of the sperm membrane herein studied as forecast of potential freezeability. From all parameters studied the ratio of percentage of C16 plasmalogens related to total phospholipids was the one with the better diagnostic value. For potentially bad freezers, the significant area under the ROC-curve was 0.74, with 75% sensitivity and 79.9% specificity for a cut off value of 26.9. Also the percentage of plasmalogens respect to total phospholipids gave good diagnostic value for bad freezers. On the other hand, the percentage of C18 fatty aldehydes related to total phospholipids of the sperm membrane properly forecasted freezeability with an area under the ROC curve of 0.70 with 70% sensitivity and 62.5% specificity for a cut off value of 0.32.

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The ether lipid-deficient mouse: Tracking down plasmalogen functions

Cited by 203 publications

References 167 publications

The Ether Lipid Precursor Hexadecylglycerol Stimulates the Release and Changes the Composition of Exosomes Derived from PC-3 Cells

The Ether Lipid Precursor Hexadecylglycerol Stimulates the Release and Changes the Composition of Exosomes Derived from PC-3 Cells

Tetrahydrobiopterin and alkylglycerol monooxygenase substantially alter the murine macrophage lipidome

Fatty acids and plasmalogens of the phospholipids of the sperm membranes and their relation with the post-thaw quality of stallion spermatozoa

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