The evaluation for generic-level monophyly of Ancyrocephalinae (Monogenea, Dactylogyridae) using ribosomal DNA sequence data

Wu, Xinyang; Zhu, Xing‐Quan; Xie, Mingquan; Li, An‐Xing

doi:10.1016/j.ympev.2007.03.025

Cited by 59 publications

(62 citation statements)

References 35 publications

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“…During the alignment analyses of products obtained by amplification of DNA from the parasite we found ~100% homology between the PCR products of all 5 pairs of primers and DNA from the original sequence of Cichlidogyrus sclerosus (Wu et al 2006). Likewise, the sequences in the DNA amplified from mucus were specific to the original sequence of the genus Cichlidogyrus (DQ537367.1) (Wu et al 2007). Alignment and matching were similar to those found in previous studies with sequences from the parasite (93 to 99%) (Mollaret et al 2000, Wu et al 2006, 2007.…”

Section: Discussionmentioning

confidence: 91%

“…The 5 primer sets amplified se quences which had ~91 to 98% homology with the original region D1 to D2 of LSU rDNA (28S gene) (DQ157660.1) GenBank (Wu et al 2006). The amplified sequences also matched the same set of parasites previously described: the LSU rDNA region of C. cubitus (Wu et al 2007).…”

Section: Sequence Matching Of Pcr Products Using Dna From Gill Mucusmentioning

confidence: 90%

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Non-lethal detection of DNA from Cichlidogyrus spp. (Monogenea, Ancyrocephalinae) in gill mucus of the Nile tilapia Oreochromis niloticus

Ek-Huchim

Jiménez-García²,

Pérez-Vega³

et al. 2012

Dis. Aquat. Org.

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Infection of Nile tilapia Oreochromis niloticus by monogeneans of the genus Cichlidogyrus is harmful. Currently, diagnosis of this infection is based on invasive techniques and the identification of isolated parasites by their morphology. To facilitate diagnosis, we have developed a non-lethal polymerase chain reaction (PCR) test for detection of Cichlidogyrus spp. DNA in the gill mucus of O. niloticus, using 5 pairs of specific primers based on Cichlidogyrus sclerosus 28S rRNA (Cicly 1 to Cicly 5) which generate fragments of approximately 188, 180, 150, 159 and 189 bp, respectively. PCR specificity was tested using genomic DNA extracted individually from 175 isolated Cichlidogyrus spp., 75 Gyrodactylus cichlidarum and 75 endopararasitic Enterogyrus spp., as well as from 75 protozoans Trichodina spp. The Cicly primers were used to detect Cichlidogyrus spp. DNA in mucus from the gills of 23 Nile tilapia confirmed to be infected with the parasite. Negative controls consisted of 45 uninfected Nile tilapia. The limit of sensitivity of the assay was 1.2 ng of purified parasite DNA. The Cicly primers did not amplify DNA from the mucus of non-infected Nile tilapia, G. cichlidarum, Trichodina spp. or Enterogyrus spp. In all cases, the sensitivity and specificity of the test were 100%. The sequences of all the amplified fragments showed a high similarity to that of the 28S rRNA region of C. sclerosus (93 to 100% identical to GenBank Accession No. DQ157660.1). We provide evidence for a safe and noninvasive DNA-based diagnostic method for the presence of Cichlidogyrus in the gill mucus of O. niloticus. KEY WORDS: Diagnosis · Ectoparasite · Monogenean · PCRResale or republication not permitted without written consent of the publisher

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Section: Discussionmentioning

confidence: 91%

Section: Sequence Matching Of Pcr Products Using Dna From Gill Mucusmentioning

confidence: 90%

Non-lethal detection of DNA from Cichlidogyrus spp. (Monogenea, Ancyrocephalinae) in gill mucus of the Nile tilapia Oreochromis niloticus

Ek-Huchim

Jiménez-García²,

Pérez-Vega³

et al. 2012

Dis. Aquat. Org.

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“…The effort to fi nd the phylogenetic position of Synodontella species among dactylogyrid parasites of Siluriformes did not provide satisfying results. The SSU and LSU rDNA regions are often used for inferring phylogenies among various groups of dactylogyrids (Pouyaud et al, 2006;Wu et al, 2007;Mendlová et al, 2010;Šimková et al, 2013;Mendoza-Palmero et al, 2015). It has been shown in a recent study of Mendoza-Palmero et al (2015) that parasites of different genera of various catfi shes are not always closely related and create paraphyletic lineages.…”

Section: S Arcopenismentioning

confidence: 99%

A revised description of Synodontella zambezensis Douëllou et Chishawa, 1995 (Monogenea: Ancyrocephalidae) from the gills of Synodontis zambezensis (Siluriformes: Mochokidae) from South Africa

et al. 2016

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SummaryThis study supplements the original description of Synodontella zambezensis Douëllou et Chishawa, 1995 and represents a new geographical record for this parasite from Synodontis zambezensis from South Africa. The revision is based on morphometric characteristics and molecular data. Characterisation of LSU, partial SSU and ITS1 rDNA represents a fi rst record of DNA sequencing for Synodontella species.

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“…To this end, we sequenced and compared the internal transcribed spacer 1 region (ITS1) of rDNA. Ten specimens were unmounted and transferred into 200 µL of TE9 buffer (500 mM TrisHCl, 200 mM EDTA, 10 mM NaCl, pH 9) (Wu et al, 2007) to clean the glycerol-gelatin from the specimens. The DNA was extracted using an Qiagen DNeasy ® Blood & Tissue Kit following the manufacturer's instructions (Qiagen, Germany).…”

Section: Molecular Datamentioning

confidence: 99%

Phenotypic plasticity in haptoral structures of Ligophorus cephali (Monogenea: Dactylogyridae) on the flathead mullet (Mugil cephalus): a geometric morphometric approach

Rodríguez-González

Míguez-Lozano

Llopis‐Belenguer

et al. 2015

International Journal for Parasitology

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Evaluating phenotypic plasticity in attachment organs of parasites can provide information on the capacity to colonize new hosts and illuminate evolutionary processes driving host specificity.We analysed the variability in shape and size of the dorsal and ventral anchors of Ligophorus cephali from Mugil cephalus by means of geometric morphometrics and multivariate statistics. We also assessed the morphological integration between anchors and between the roots and points in order to gain insight into their functional morphology. Dorsal and ventral anchors showed a similar gradient of overall shape variation, but the amount of localized changes was much higher in the former. Statistical models describing variations in shape and size revealed clear differences between anchors. The dorsal anchor/bar complex seems more mobile than the ventral one in Ligophorus, and these differences may reflect different functional roles in attachment to the gills. The lower residual variation associated with the ventral anchor models suggests a tighter control of their shape and size, perhaps because these anchors seem to be responsible for firmer attachment and their size and shape would allow more effective responses to characteristics of the microenvironment within the individual host. Despite these putative functional differences, the high level of morphological integration indicates a concerted action between anchors. In addition, we found a slight, although significant, morphological integration between roots and points in both anchors, which suggests that a large fraction of the observed phenotypic variation does not compromise the functional role of anchors as levers. Given the low level of genetic variation in our sample, it is likely that much of the morphological variation reflects host-driven plastic responses. This supports the hypothesis of monogenean specificity through host-switching and rapid speciation. The present study demonstrates the potential of geometric morphometrics to provide new and previously unexplored insights into the functional morphology of attachment and evolutionary processes of host-parasite coevolution.4

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The evaluation for generic-level monophyly of Ancyrocephalinae (Monogenea, Dactylogyridae) using ribosomal DNA sequence data

Cited by 59 publications

References 35 publications

Non-lethal detection of DNA from Cichlidogyrus spp. (Monogenea, Ancyrocephalinae) in gill mucus of the Nile tilapia Oreochromis niloticus

Non-lethal detection of DNA from Cichlidogyrus spp. (Monogenea, Ancyrocephalinae) in gill mucus of the Nile tilapia Oreochromis niloticus

A revised description of Synodontella zambezensis Douëllou et Chishawa, 1995 (Monogenea: Ancyrocephalidae) from the gills of Synodontis zambezensis (Siluriformes: Mochokidae) from South Africa

Phenotypic plasticity in haptoral structures of Ligophorus cephali (Monogenea: Dactylogyridae) on the flathead mullet (Mugil cephalus): a geometric morphometric approach

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