The expression and clinical significance of three lncRNAs in patients with a missed abortion

Abstract

Missed abortions are common complications that occur in early pregnancy, and impaired trophoblast functions have been indicated to be associated with their pathogenesis. The long noncoding RNAs (lncRNAs) Metastasis Associated Lung Adenocarcinoma Transcript 1 ( MALAT1) , HOX Transcript Antisense RNA ( HOTAIR) and Maternally expressed gene 3 ( MEG3) have been demonstrated to serve a crucial regulatory role in the mobility of trophoblast cells and embryo implantation. However, the expression profile and role of each of these three lncRNAs in patients with a missed abortion remain unclear. The expression of MALAT1 , HOTAIR , and MEG3 in decidual and villous tissues from 26 patient exhibiting a missed abortion and 26 healthy controls was detected using reverse-transcription quantitative PCR. Serum TNF-α, IL-1β, IL-6 and IL-10 levels were measured using ELISA, and serum estradiol and progesterone levels were measured with electrochemiluminescence immunoassays. Additionally, the correlations between lncRNA expression and the levels of cytokines and hormones were further analyzed. MALAT1 , HOTAIR and MEG3 expression was significantly higher in villous tissues of patients exhibiting a missed abortion compared with healthy controls. MALAT1 expression was higher in decidual tissues of patients exhibiting a missed abortion compared with healthy controls. Serum IL-10 levels were significantly lower in patients exhibiting a missed abortion compared with healthy controls. Serum estradiol and progesterone levels were significantly lower in the group of patients exhibiting a missed abortion compared with the control group. Furthermore, MALAT1 expression in villous tissue was inversely related to serum progesterone levels. The results of the current study suggest that MALAT1 may be associated with the pathogenesis of missed abortions.

Genetic variants of HOTAIR are associated with susceptibility to recurrent spontaneous abortion: A preliminary case–control study

“…Luo et al exhibited higher expression of HOTAIR in RSA patients compared with healthy controls 63 . Interestingly, it is reported that HOTAIR can be suppressed in RSA 64 .…”

Potential Contribution of the Uterine Microbiome in Early Missed Abortion

“…Missed abortion is a special type of spontaneous abortion which accompany serious complication endanger the reproductive healthy [20]. Previous studies on missed abortion focused on chromosome abnormalities [21], uterine artery blood ow resistance, angiogenesis [22], immune in ammationrelated [23,24], etc. In recent years, with the further study of the human microbiome, researchers gradually realized that from the surface of human body to the intestinal ora, all of them have an impact on the ecological balance of human body and the occurrence and development of diseases.…”

Significance of Sex Differences in ncRNAs Expression and Function in Pregnancy and Related Complications

“…LncRNAs modulate a wide variety of biological processes, such as cellular differentiation, maintenance of stem cell pluripotency, and development of tissues and organs, contributing to the onset of human diseases [ 17 , 43 ]. They are implicated in different pregnancy-related complications, including preeclampsia [ 44 ], GDM [ 45 ], intrauterine growth restriction (IUGR) [ 46 ], and missed abortions [ 47 ].…”