The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration

Akmal, Muslim; Siregar, Tongku Nizwan; Wahyuni, Sri; Hamny,; Nasution, Mustafa Kamal; Indriati, Wiwik; Panjaitan, Budianto; Aliza, Dwinna

doi:10.14202/vetworld.2016.1001-1005

Cited by 3 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Testosterone is a crucial steroid hormone for male reproductive development and function [25]. It is very important for maintaining spermatogenesis process [26] and male fertility [27].…”

Section: Discussionmentioning

confidence: 99%

The concentration of testosterone, pituitary adenylate cyclase-activating polypeptide, and protamine 1 in the serum of male chicken following administration of epididymis and testicular extracts and their combination

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background and Aim: Testis and epididymis are male reproductive organs that play an important role in spermatogenesis. These two organs are rich in the content of hormones and other molecules needed in the process of spermatogenesis which affect the quality of the spermatozoa. The objective of this study was to examine the effect of the administration of epididymis and testicular extracts and their combination on testosterone, pituitary adenylate cyclase-activating polypeptide (PACAP), and protamine 1 (PRM1) concentrations in the serum of male chicken. Materials and Methods: Twenty male chickens (broiler strain Cp707), aged 3 weeks and weighing 800-1000 g, were randomly divided into four different groups including a control group (T0) = injected with 1 ml normal saline and treatment groups: T1 = injected with 1 ml epididymis extract, T2 = injected with 1 ml testicular extract, and T3 = injected with a combination of 1 ml epididymis + 1 ml testicular extract. The experiment was conducted for 13 days and at the end of the study (day 14), the chickens were sacrificed to obtain the serum. Furthermore, the concentrations of testosterone, PACAP, and PRM1 were then measured by using an enzyme-linked immunosorbent assay technique. Results: The concentrations of PACAP and PRM1 did not show a significant difference between treatment groups (T1, T2, and T3) and control group (T0) (p>0.05). However, the concentration of testosterone showed a significantly higher difference in a group injected with a combination of 1 ml epididymis and 1 ml testicular extracts (T3) compared to the control group (T0) (p<0.05). Conclusion: The administration of epididymis and testicular extracts and their combination did not affect the increase of PACAP and PRM1 concentration. However, a combination of these extracts significantly affects the increase of testosterone concentration in the serum of male chicken.

show abstract

“…Testosterone is a crucial steroid hormone for male reproductive development and function [25]. It is very important for maintaining spermatogenesis process [26] and male fertility [27].…”

Section: Discussionmentioning

confidence: 99%

The concentration of testosterone, pituitary adenylate cyclase-activating polypeptide, and protamine 1 in the serum of male chicken following administration of epididymis and testicular extracts and their combination

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, testosterone also plays an important role in spermatogenesis, especially in the spermiation stage [8][9][10]. Disruption of the spermatogenesis process might cause infertility in male animals [11].…”

Section: Introductionmentioning

confidence: 99%

Identification of Active Compounds of Kepok Banana Peel and the Effect on Testosterone Concentration in Male Rats with High-Fat Diet

et al. 2020

Self Cite

View full text Add to dashboard Cite

Our body needs an adequate supply of antioxidants to maintain physiological functions. Antioxidant compounds are found in natural plants, including inside of kepok bananas peel. This research was conducted to determine the potential of kepok banana peels to maintain testosterone concentration in male rats (Rattus norvegicus) with a high-fat diet. This study used 20 male rats 2.5-3 months old, which grouped into 5 groups: normal group given standard feed (K1), negative control group given high-fat diet (K2), positive control group given high-fat diet and simvastatin (K3), high-fat diet group and banana peel extract administered at 100 mg/kg BW (K4), and high-fat diet group and banana peel extract administered at 200 mg/ kg BW (K5). The blood serum was collected after 60 days of the treatment. The blood serum testosterone levels were measured by enzyme-linked immunosorbent assay (ELISA) method at a wavelength of 450 nm. The major findings of this study were that there is a significant difference between testosterone concentrations by the K4 group compared to K2 and K5 groups (p<0,05). The result suggests that the dose 100 mg/kg BW of banana peel extract has the potential to maintain the testosterone concentration in male rats with a high-fat diet.

show abstract

“…Androgen is a steroid hormone that serves as a precursor for testosterone production (major hormone of androgen). This hormone is required in the process of spermatogenesis [ 5 , 6 ]. This hormone is also essential for normal expression of phenotypic expressions of the male.…”

Section: Introductionmentioning

confidence: 99%

The concentration of androgen receptor and protein kinase A in male chicken following the administration of a combination of the epididymis and testicular extracts

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background and Aim: Testis (T) and epididymis (E) are waste from the abattoir that is rarely used. In fact, both organs contain important chemicals needed for spermatogenesis (e.g., hormones, proteins, and other molecules). Therefore, administration of a combination of testis and epididymis (CTE) extracts may activate androgen receptors (AR) and protein kinase A (PKA) molecules that play a prominent role in spermatogenesis. We, therefore, aimed at investigating the influence of the CTE extracts on the concentration of AR and PKA in male chicken. Materials and Methods: This study used a completely randomized design with four treatment groups (K0, K1, K2, and K3) and five replications per group. K0 is a control group that received 1 mL normal saline, whereas K1, K2, and K3 are the test groups that received 1, 2, and 3 mL of CET extracts, respectively. Twenty male chickens (strain: broiler Mb 89), 3 weeks of age, weighing 500-700 g were used. We administered the injections in a 13-day period and on the 14th day; we collected and processed blood samples as serum to measure the AR and PKA concentrations using commercial chicken AR and PKA enzyme-linked immunosorbent assay kits, respectively. We performed analyses by analysis of variance using SPSS 20.0. Results: The AR concentrations in K1, K2, and K3 groups increased by 4.26%, 10.97%, and 28.04%, respectively, compared to the K0 (control group). However, this increase was not significantly different between the groups (p>0.05). Moreover, the PKA concentrations increased by 2.97%, 2.60%, and 4.08% in K1, K2, and K3 groups, respectively, compared to the control group. However, this increase was not significantly different between the groups as well (p>0.05). Conclusion: The CTE extracts tended to increase the AR and PKA concentrations even though it is not significant. Therefore, it needs further study when using the CTE extracts for spermatogenesis in male chicken.

show abstract

The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration

Cited by 3 publications

References 27 publications

The concentration of testosterone, pituitary adenylate cyclase-activating polypeptide, and protamine 1 in the serum of male chicken following administration of epididymis and testicular extracts and their combination

The concentration of testosterone, pituitary adenylate cyclase-activating polypeptide, and protamine 1 in the serum of male chicken following administration of epididymis and testicular extracts and their combination

Identification of Active Compounds of Kepok Banana Peel and the Effect on Testosterone Concentration in Male Rats with High-Fat Diet

The concentration of androgen receptor and protein kinase A in male chicken following the administration of a combination of the epididymis and testicular extracts

Contact Info

Product

Resources

About