The expression of miR-513c and miR-3163 was downregulated in tumor tissues compared with normal adjacent tissue of patients with breast cancer

Abstract

Background Breast cancer (BC) is the most invasive cancer with different subtypes that its metabolism is unique compared with normal cells. Glutamine is considered critical nutrition that many cancer cells, particularly BC cells, are dependent on it for growth and proliferation. Therefore, targeting glutamine metabolism, especially enzymes that are related to this pathway, can be beneficial to design anti-cancer agents. Recent evidence has shown that microRNAs (miRNAs), with a short length and single-strand properties, play a prominent role in regulating the genes related to glutamine metabolism, which may control the development of cancer. Methods In silico analysis confirmed that miR-513c and miR-3163 might be involved in glutamine metabolism. The expression level of these two miRNAs was evaluated in eighty BC tissues and normal adjacent tissues. Furthermore, GSE38167, GSE38867, GSE42128, GSE45666, and GSE53179 were employed from gene expression omnibus (GEO). The Limma package was utilized to identify differentially expressed miRNAs (DEMs) of mentioned datasets to evaluate miR-513c and miR-3163 expression. Further, in silico analysis was utilized to predict the potential biological processes and molecular pathways of miR-513c and miR-3163, based on their target genes. Results In silico studies revealed top categories of biological processes and cellular pathways that might play a critical role in metabolism reprogramming and cancer development and were target genes for miR-513c and miR-3163. The current study showed that miR-513c (p value = 0.02062 and FC = − 2.3801) and miR-3163 (p value = 0.02034 and FC = − 2.3792) were downregulated in tumor tissues compared to normal adjacent tissues. The analysis of GEO microarray datasets showed that miR-513c was downregulated in GSE38167, GSE38867, GSE42128, GSE45666 and GSE53179, whereas there was a significant downregulation of miR-3163 in only two studies, including GSE38867 and GSE42128 that they were in accordance with our experimental results. Furthermore, the subgroup analysis did not show any substantial relationship between expression levels of these two miRNAs and factors such as age, family history of cancer, and abortion history. Conclusion MiR-513c and miR-3163 were downregulated in BC tissues, which might serve as tumor suppressors. They are suggested as potential therapeutic targets for patients with BC.

Hsa-mir-3163 and CCNB1 may be potential biomarkers and therapeutic targets for androgen receptor positive triple-negative breast cancer

“…Functionally, hsa-miR-3163 played as a tumor suppressor. A recent study in breast cancer indicated that MiR-513c and miR-3163 were downregulated in tumor tissues, which might serve as tumor suppressors [45]. This is in line with the results of our research.…”

Cyclin‐dependent kinase 5 promotes the growth of tongue squamous cell carcinoma through the microRNA 513c‐5p/cell division cycle 25B pathway and is associated with a poor prognosis

“…However, studies of liver cancer and cervical cancer have indicated that a low level of miR513c-5p expression is related to higher rates of local tissue invasion, lymphatic metastasis, and distant metastasis. 14,39,40 In summary, the miR513c-5p/CDK5/CDC25B axis in TSCC cells may be highly regulated. As an oncogene, CDK5 drives the occurrence and development of TSCC tumors and might serve as a biomarker for diagnosing TSCC and a molecular target for treating the disease.…”

“…Few studies have investigated the miR513c‐5p gene, which is upstream of CDK5. However, studies of liver cancer and cervical cancer have indicated that a low level of miR513c‐5p expression is related to higher rates of local tissue invasion, lymphatic metastasis, and distant metastasis 14,39,40 …”

MEX3A promotes nasopharyngeal carcinoma progression via the miR-3163/SCIN axis by regulating NF-κB signaling pathway

“…6A ). Considering that decreased hsa-miRNA-3163 expression was previously reported in NPC tissues [ 26 ], we selected hsa-miRNA-3163 for further study. We also verified the MEX3A knockdown led to increased expression of miRNA-3163 in NPC cells ( P < 0.001, Fig.…”

“…Emerging evidence indicates that miRNA-3163 is downregulated and functions as a tumor suppressor in various cancers [ 26 , 34 , 35 ]. A previous study reported that miRNA-3163 exerted its tumor suppression function by downregulating TWIST-1 in NPC [ 35 ].…”