The expression of soluble functional α7-nicotinic acetylcholine receptors in E. coli and its high-affinity binding to neonicotinoid pesticides

Xiang, Dandan; Xu, Xiao‐Jun; Zhou, Qiaoling; Yan, Ru; Chen, Mengli; Guo, Yirong; Zhu, Guonian

doi:10.1016/j.pestbp.2020.02.002

Cited by 6 publications

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The α7 nACh receptor, which is expressed in SH-SY5Y cells [ 31 ], evokes the highest Ca 2+ transients in neurons to keep the intracellular concentration of the cation to an optimum [ 46 ]. IMI and ACE differently modulate ACh-induced Ca 2+ currents on α7 nACh receptors [ 47 , 48 ], which would explain why IMI was more cytotoxic than ACE, while only IMI at low concentrations promoted cell proliferation in immature SH-SY5Y cells [ 49 ]. In addition, ACE and IMI may also act like “orthosteric” agonists producing a rapid desensitization of α7 nACh receptors (unpublished observations), which is likely to produce oxidative stress [ 50 , 51 ] in differentiating SH-SY5Y cells.…”

Section: Discussionmentioning

confidence: 99%

Neurocytotoxicity of imidacloprid- and acetamiprid-based comercial insecticides over the differentiation of SH-SY5Y neuroblastoma cells

Ramírez-Cando

Guzmán-Vallejos

Aguayo

et al. 2023

Heliyon

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Neurocytotoxicity of imidacloprid- and acetamiprid-based comercial insecticides over the differentiation of SH-SY5Y neuroblastoma cells

Ramírez-Cando

Guzmán-Vallejos

Aguayo

et al. 2023

Heliyon

View full text Add to dashboard Cite

“…Imidacloprid [1-(6-chloro-3-pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine] is a novel neonicotinoid synthetic insecticide that is widely used for crop protection and pest prevention due to its broad-spectrum and high insecticidal activity [42][43][44]. One major advantage of imidacloprid is its selective action on the central nervous system of insects (post-synaptic nicotinic acetylcholine receptors) [45][46][47]. However, some studies have indicated that the use of neonicotinoid insecticides, such as imidacloprid, is a key factor in the decline in non-target terrestrial insect biodiversity in the environment [48][49][50][51][52].…”

Section: Introductionmentioning

confidence: 99%

Effects of Multiple Environmental Stressors on Zoobenthos Communities in Shallow Lakes: Evidence from a Mesocosm Experiment

Xu,

Su,

Zhang

et al. 2023

Animals

View full text Add to dashboard Cite

Multiple stressors, including climate change, eutrophication, and pesticide contamination, are significant drivers of the decline in lake zoobenthos. Zoobenthos play a crucial role in aquatic ecosystems, impacting energy dynamics, nutrient cycling, and sediment degradation. However, these stressors have led to a decrease in the abundance and diversity of zoobenthos, resulting in notable changes in species composition and structure. Eutrophication typically increases zoobenthos abundance while reducing taxonomic diversity. Climate change, such as warming and heatwaves, also affects the zoobenthos community structure, with different species exhibiting varying levels of adaptability to temperature changes. Additionally, pesticides like imidacloprid have negative effects on the survival and growth of zoobenthos. However, the interactions between imidacloprid and other stressors remain understudied. Here, we used 48 mesocosms (2500 L) to simulate shallow lakes. We combined nutrient loading, sustained warming, and the imidacloprid pesticide to test how these stressors interactively influence the survival and community of zoobenthos. The experimental results demonstrate that elevated temperatures have a significant impact on aquatic benthic organisms under different treatment conditions. The increase in temperature led to a notable rise in species richness and α-diversity, primarily attributed to the stimulation of metabolic activities in zoobenthos, promoting their growth and reproduction. This finding underscores the potential influence of climate change on aquatic benthic ecosystems, particularly in terms of its promoting effect on α-diversity. However, it is essential to note that elevated temperatures also reduced β-diversity among different sites, implying a potential trend toward homogenization in zoobenthos communities under warmer conditions. Moreover, this study revealed the interactive effects of multiple stressors on the diversity of aquatic benthic communities. Specifically, the pesticide imidacloprid’s impact on zoobenthos is not isolated but demonstrates complex effects within various treatment interactions. In the presence of both temperature elevation and the addition of imidacloprid, the presence of imidacloprid appears to counteract the adverse effects of temperature elevation, resulting in increased species diversity. However, when imidacloprid coincides with nutrient input, it significantly affects α-diversity negatively. These findings highlight the complexity of zoobenthos responses to multiple stressors and how these factors influence both α-diversity and β-diversity. They provide valuable insights for further research on the conservation and management of ecosystems.

show abstract

Catalytic hairpin-assembled and DNAzyme-driven DNA walker integrated hybridization chain reaction to construct a label-free electrochemical aptasensor for the detection of acetamiprid

Zhang,

Zhu,

Yang

et al. 2024

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

The expression of soluble functional α7-nicotinic acetylcholine receptors in E. coli and its high-affinity binding to neonicotinoid pesticides

Cited by 6 publications

References 33 publications

Neurocytotoxicity of imidacloprid- and acetamiprid-based comercial insecticides over the differentiation of SH-SY5Y neuroblastoma cells

Neurocytotoxicity of imidacloprid- and acetamiprid-based comercial insecticides over the differentiation of SH-SY5Y neuroblastoma cells

Effects of Multiple Environmental Stressors on Zoobenthos Communities in Shallow Lakes: Evidence from a Mesocosm Experiment

Catalytic hairpin-assembled and DNAzyme-driven DNA walker integrated hybridization chain reaction to construct a label-free electrochemical aptasensor for the detection of acetamiprid

Contact Info

Product

Resources

About