The Expression of the RUVBL1 Component of the R2TP Complex Correlates with Poor Prognosis in DLBCL

Lone, Moien Rasheed; Shadang, Mahaiwon; Akhter, Qulsum; Kumar, M. Vijay; Mallick, Saumyaranjan; Gogia, Ajay; Nilima, Nilima; Chauhan, Shyam S.; Mir, Riyaz Ahmad

doi:10.1159/000520723

Cited by 4 publications

(4 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RUVBL1, or RuvB-like AAA ATPase 1, has been associated with poor prognosis and a higher relapse rate in patients with diffuse large B-cell lymphoma (17). In addition, high expression of RuvBL1 in lung cancer was associated with a poor overall prognosis (18).…”

Section: Discussionmentioning

confidence: 99%

Potential effects of POLR2H and DYNC1I2 on the immunity and prognosis of neuroblastoma

et al. 2022

Preprint

View full text Add to dashboard Cite

Objective: The present study utilized bioinformatics techniques and data from the GEO, TARGET, and ArrayExpress databases to compare gene expression in INSS4 and INSS1 neuroblastomas (NBs), thereby identifying metabolites with different levels of expression and predicting the prognosis of patients with NB.METHODS: Genes of patients with INSS4 and INSS1 NBs from the GEO database were screened, with those having | log2fold change (FC) | >3 and adjusted P <0.05 defined as being differentially expressed. These differentially expressed genes (DEGs) were screened to obtain clinical data and RNA sequence datasets from NB patients in the TARGET database. Univariate Cox proportional hazards regression analysis identified prognosis-related genes, which were incorporated into a prognosis model. Based on median risk scores, these patients were divided into high and low-risk groups. Their survival rates were compared, and ROC curves were used to analyze predictive values for NB. NB patients were also divided into two clusters by consensus clustering based on levels of POLR2H and DYNC1I2 expression. Immune infiltration analyses were performed using GSEA, ESTIMATE, CIBERSORT, and ssGSEA. Tumor tissue of 17 NB patients was used for experimental verification and their survival was compared.Result: Analysis of three datasets identified 62 up-regulated genes and 163 down-regulated genes. The prognostic model predicted that the areas under the 3-year and 5-year survival curves were 0.786 and 0.817, respectively. Levels of expression of POLR2H and DYNC1I2 accounted for the highest percentage of risk scores and were included in follow-up analysis. Samples were consistently clustered according to their expression matrix. POLR2H was more highly expressed in cluster 2, whereas DYNC1I2 was more highly expressed in cluster 1. The survival rate of cluster 1 was significantly higher than that of cluster 2. Experimental verification in 17 NB patients showed that these patients could also be divided into two groups, which differed significantly in mortality hazard ratio (HR 9.37 P<0.05).Conclusion: The expression of POLR2H and DYNC1I2 affects the immune microenvironment of NB and can affect patient prognosis. These factors can be used to refine clinical groupings, guide personalized treatment, and suggest new methods for the diagnosis and treatment of NB.

show abstract

Section: Discussionmentioning

confidence: 99%

Potential effects of POLR2H and DYNC1I2 on the immunity and prognosis of neuroblastoma

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Potential effects of POLR2H and DYNC1I2 on the immunity and prognosis of neuroblastoma

Lü

et al. 2022

Preprint

View full text Add to dashboard Cite

Objective The present study utilized bioinformatics techniques and data from the GEO, TARGET, and ArrayExpress databases to compare gene expression in INSS4 and INSS1 neuroblastomas (NBs), thereby identifying metabolites with different levels of expression and predicting the prognosis of patients with NB. METHODS Genes of patients with INSS4 and INSS1 NBs from the GEO database were screened, with those having ཛྷlog2fold change (FC)ཛྷ>3 and adjusted P < 0.05 defined as being differentially expressed. These differentially expressed genes (DEGs) were screened to obtain clinical data and RNA sequence datasets from NB patients in the TARGET database. Univariate Cox proportional hazards regression analysis identified prognosis-related genes, which were incorporated into a prognosis model. Based on median risk scores, these patients were divided into high and low-risk groups. Their survival rates were compared, and ROC curves were used to analyze predictive values for NB. NB patients were also divided into two clusters by consensus clustering based on levels of POLR2H and DYNC1I2 expression. Immune infiltration analyses were performed using GSEA, ESTIMATE, CIBERSORT, and ssGSEA. Tumor tissue of 17 NB patients was used for experimental verification and their survival was compared. Result Analysis of three datasets identified 62 up-regulated genes and 163 down-regulated genes. The prognostic model predicted that the areas under the 3-year and 5-year survival curves were 0.786 and 0.817, respectively. Levels of expression of POLR2H and DYNC1I2 accounted for the highest percentage of risk scores and were included in follow-up analysis. Samples were consistently clustered according to their expression matrix. POLR2H was more highly expressed in cluster 2, whereas DYNC1I2 was more highly expressed in cluster 1. The survival rate of cluster 1 was significantly higher than that of cluster 2. Experimental verification in 17 NB patients showed that these patients could also be divided into two groups, which differed significantly in mortality hazard ratio (HR 9.37 P < 0.05). Conclusion The expression of POLR2H and DYNC1I2 affects the immune microenvironment of NB and can affect patient prognosis. These factors can be used to refine clinical groupings, guide personalized treatment, and suggest new methods for the diagnosis and treatment of NB.

show abstract

“…The most virulent malignancy of mature B-lymphocytes is diffuse large B-cell lymphoma (DLBCL) which is commonly found in non-Hodgkin’s lymphoma of adults with heterogenous genetic disorders (Lone et al 2022 ). This disorder is perpetuated by B-cells of the germinal center (GC) and contributes to cancer development attributes such as high cell growth rate and genomic instability (Hanahan and Weinberg 2011 ).…”

Section: The Role Of R2tp In the Development Of Cancermentioning

confidence: 99%

“…Deregulation of tumor suppressor pathways including p53 and pRb is responsible for rapid proliferation and transformation (Giacinti and Giordano 2006 ). In addition, the function of Rb and p53 is activated by the R2TP complex, suggesting that any alterations in any of the members of the complex can drive cells to malignancy (Lone et al 2022 ).…”

Section: The Role Of R2tp In the Development Of Cancermentioning

confidence: 99%

The multi-faceted roles of R2TP complex span across regulation of gene expression, translation, and protein functional assembly

Luthuli,

Shonhai

2023

Biophys Rev

View full text Add to dashboard Cite

Macromolecular complexes play essential roles in various cellular processes. The assembly of macromolecular assemblies within the cell must overcome barriers imposed by a crowded cellular environment which is characterized by an estimated concentration of biological macromolecules amounting to 100–450 g/L that take up approximately 5–40% of the cytoplasmic volume. The formation of the macromolecular assemblies is facilitated by molecular chaperones in cooperation with their co-chaperones. The R2TP protein complex has emerged as a co-chaperone of Hsp90 that plays an important role in macromolecular assembly. The R2TP complex is composed of a heterodimer of RPAP3:P1H1DI that is in turn complexed to members of the ATPase associated with diverse cellular activities (AAA +), RUVBL1 and RUVBL2 (R1 and R2) families. What makes the R2TP co-chaperone complex particularly important is that it is involved in a wide variety of cellular processes including gene expression, translation, co-translational complex assembly, and posttranslational protein complex formation. The functional versatility of the R2TP co-chaperone complex makes it central to cellular development; hence, it is implicated in various human diseases. In addition, their roles in the development of infectious disease agents has become of interest. In the current review, we discuss the roles of these proteins as co-chaperones regulating Hsp90 and its partnership with Hsp70. Furthermore, we highlight the structure–function features of the individual proteins within the R2TP complex and describe their roles in various cellular processes.

show abstract

The Expression of the RUVBL1 Component of the R2TP Complex Correlates with Poor Prognosis in DLBCL

Cited by 4 publications

References 47 publications

Potential effects of POLR2H and DYNC1I2 on the immunity and prognosis of neuroblastoma

Potential effects of POLR2H and DYNC1I2 on the immunity and prognosis of neuroblastoma

Potential effects of POLR2H and DYNC1I2 on the immunity and prognosis of neuroblastoma

The multi-faceted roles of R2TP complex span across regulation of gene expression, translation, and protein functional assembly

Contact Info

Product

Resources

About