The expression pattern of aldehyde dehydrogenase 1 (ALDH1) is an independent prognostic marker for low survival in colorectal tumors

Vogler, T; Kriegl, Lydia; Horst, David; Engel, Jutta; Sagebiel, Sabine; Schäffauer, A.J.; Kirchner, Thomas; Jung, Andreas

doi:10.1016/j.yexmp.2011.10.010

Cited by 28 publications

(26 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…35 In a large study including 1420 patients with colorectal cancer of all stages, no significant correlation could be found between ALDH expression and survival, 36 whereas the ALDH1 expression pattern had a significant impact upon survival for G2 T3N0M0 colorectal cancer in another study. 37 Our findings suggest that upper urinary tract urothelial cell carcinoma contains ALDH1-positive cancer stem-like cells/tumor-initiating cells like bladder cancer, and that these cells are associated with survival or life-threatening disease, as 62% of the patients with intravesical recurrence were alive without any other recurrence.…”

Section: Discussionmentioning

confidence: 68%

Prognostic impact of the expression of ALDH1 and SOX2 in urothelial cancer of the upper urinary tract

et al. 2013

View full text Add to dashboard Cite

Aldehyde dehydrogenase 1 (ALDH1) and sex determining region-Y-related high mobility group box 2 (SOX2) have been identified as putative cancer stem-like cell/tumor-initiating cell markers in various cancer tissues. The aim of this study was to elucidate the prognostic impact of these putative cancer stem-like cell/tumorinitiating cell markers in upper urinary tract urothelial cell carcinoma. Immunohistochemical staining for ALDH1 and SOX2 was carried out on archival specimens from 125 patients with upper urinary tract urothelial cell carcinoma who underwent radical nephroureterectomy. The prognostic value of ALDH1 and SOX2 expression and other clinicopathological features was evaluated. On univariate analysis, tumor grade, pathological T stage, pathological N stage, lymphovascular invasion, ALDH1 expression and SOX2 expression were associated with a poor prognosis. On multivariate analysis, the independent factors of prognosis were tumor grade (P ¼ 0.014), pathological N stage (P ¼ 0.005) and ALDH1 expression (P ¼ 0.002). In subgroup analysis, those subgroups with no positive, one positive or two positive results in immunohistochemistry for ALDH1 and SOX2 expression had estimated 5-year cancer-specific survival rates of 80%, 49% and 22%, respectively (Po0.001). Neither ALDH1 nor SOX2 expression correlated with intravesical recurrence after radical nephroureterectomy. These findings suggest that cancer stem-like cells/tumor-initiating cells are linked to more aggressive behavior of upper urinary tract urothelial cell carcinoma, supporting the current cancer stem cell hypothesis. Thus, therapeutic targeting of cancer stem-like cells/tumor-initiating cells in upper urinary tract urothelial cell carcinoma is a future possibility.

show abstract

Section: Discussionmentioning

confidence: 68%

Prognostic impact of the expression of ALDH1 and SOX2 in urothelial cancer of the upper urinary tract

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Knockdown of ALDH1 in SiHa-ALDH1-positive cells abolished the ability of these cells to form tumors in nude mice. ALDH1 expression has been previously used as a prognostic marker, a diagnostic marker and a marker or drug resistance in a number of tumors (Schnier et al, 1999;Vogler et al, 2011;Heerma van Voss et al, 2011;Wang et al, 2011;Moreb et al, 2012;Wang et al, 2012). However, to the best of our knowledge, we are the first to demonstrate the role of ALDH1 in tumorigenesis and metastasis in cervical cancer.…”

Section: Discussionmentioning

confidence: 86%

“…ALDH1 has been proposed as a diagnostic marker, a therapeutic target as well as a prognostic marker in a number of cancers (Heerma et al, 2011;Vogler et al, 2011;Wang et al, 2012). However, its functional role remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Expression and Functional Role of ALDH1 in Cervical Carcinoma Cells

Rao¹,

Yao²,

Zhang³

et al. 2012

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Tumor formation and growth is dictated by a very small number of tumor cells, called cancer stem cells, which are capable of self-renewal. The genesis of cancer stem cells and their resistance to conventional chemotherapy and radiotherapy via mechanisms such as multidrug resistance, quiescence, enhanced DNA repair abilities and anti-apoptotic mechanisms, make it imperative to develop methods to identify and use these cells as diagnostic or therapeutic targets. Aldehyde dehydrogenase 1 (ALDH1) is used as a cancer stem cell marker. In this study, we evaluated ALDH1 expression in CaSki, HeLa and SiHa cervical cancer cells using the Aldefluor method to isolate ALDH1-positive cells. We showed that higher ALDH1 expression correlated with significantly higher rates of cell proliferation, microsphere formation and migration. We also could demonstrate that SiHa-ALDH1-positive cells were significantly more tumorigenic compared to SiHa-ALDH1-negative cells. Similarly, SiHa cells overexpressing ALDH1 were significantly more tumorigenic and showed higher rates of cell proliferation and migration compared to SiHa cells where ALDH1 expression was knocked down using a lentivirus vector. Our data suggested that ALDH1 is a marker of cervical cancer stem cells and expand our understanding of its functional role.

show abstract

“…Expression of the ALDH1 family in colon cancer cell lines and patient samples have been utilized by several lab groups to identify and isolate CSCs. 2,11,21,22 While many isoforms have been identified as biomarkers and indicators of tumorigenicity and cancer progression, several other isoforms still remain to be investigated.…”

Section: Introductionmentioning

confidence: 99%

The Proportion of ALDEFLUOR-Positive Cancer Stem Cells Changes with Cell Culture Density Due to the Expression of Different ALDH Isoforms

Opdenaker

Modarai

Boman³

2015

Cancer Stud Mol Med Open J

View full text Add to dashboard Cite

A significant number of discrepancies exist within the literature regarding ALDEFLUOR-positive stem cell populations in cell lines. We hypothesized that these inconsistencies resulted from differences in culture conditions, particularly cell density. We cultured several colon cancer cell lines (N=8) at high and low densities and found a significant decrease in ALDEFLUOR-positive cell populations at high density. However, we found no changes in the CD166-positive stem cell population, self-renewal, or cell cycle distribution of cells cultured at different densities. Interestingly, when we sorted both ALDEFLUOR positive and negative populations from the different density cultures, we identified a significant number of Aldehyde dehydrogenase (ALDH) isoforms whose expression was decreased in ALDEFLUOR-positive stem cells cultured at high density. This novel finding suggests that multiple ALDH isoforms contribute to ALDEFLUOR activity in colon cancer stem cells and decreases in ALDEFLUOR-positive stem cells at high cell density are due to decreased expression of multiple ALDH isoforms. Thus, designing therapeutics to target ALDEFLUOR-positive cancer stem cells may require inhibition of multiple ALDH isoforms.

show abstract

The expression pattern of aldehyde dehydrogenase 1 (ALDH1) is an independent prognostic marker for low survival in colorectal tumors

Cited by 28 publications

References 35 publications

Prognostic impact of the expression of ALDH1 and SOX2 in urothelial cancer of the upper urinary tract

Prognostic impact of the expression of ALDH1 and SOX2 in urothelial cancer of the upper urinary tract

Expression and Functional Role of ALDH1 in Cervical Carcinoma Cells

The Proportion of ALDEFLUOR-Positive Cancer Stem Cells Changes with Cell Culture Density Due to the Expression of Different ALDH Isoforms

Contact Info

Product

Resources

About