The expression pattern of the Picea glauca Defensin 1 promoter is maintained in Arabidopsis thaliana, indicating the conservation of signalling pathways between angiosperms and gymnosperms*

Germain, Hugo; Lachance, Denis; Pelletier, G.; Fossdal, Carl Gunnar; Solheim, Halvor; Séguin, Armand

doi:10.1093/jxb/err303

Cited by 36 publications

(21 citation statements)

References 48 publications

(69 reference statements)

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“…Although the activity pattern of several plant promoters is conserved even between angiosperms and gymnosperms (Germain et al 2012) indicating the universal functionality of CREs, differential performance has also been reported (Terzaghi andCashmore 1995, Hernandez-Garcia andFiner 2014). Thus, taking into account the different types of structure and development of flowers and fruits between A. thaliana and M. truncatula, together with the above-mentioned discrepancies, we have considered the necessity to study the activity of the pSTs in transgenic plants of M. truncatula.…”

Section: Introductionmentioning

confidence: 99%

Promoter activity of genes encoding the Specific Tissue protein family in the reproductive organs of Medicago truncatula

Albornos¹,

Martín²,

Labrador³

et al. 2019

Biol. plant.

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The "Specific Tissue" (ST) are proteins of unknown function present only in some plant families, mainly Fabaceae and Asteraceae. They are included in the PF10950 protein family and characterized by the presence of at least one domain of unknown function (DUF)2775. In this work we studied the involvement of the six members of the Medicago truncatula ST family (ST1 to ST6) in the development of flowers, fruits, and seeds by analysing the activity of their promoters (pST) after the construction of M. truncatula transgenic plants expressing the b-glucuronidase (GUS) reporter gene under the control of the six pSTs. The GUS activity was analysed in whole flowers and fruits and also in histological sections of these organs. The pST expression in the reproductive organs was mainly associated with the vascular bundles, especially throughout fruit development. These results pointed to an important role of ST proteins during the reproductive development stage, related to nutrient mobilization during the fruit and seed formation, that could be facilitated by their presence in the pod vascular bundles, as well as in the connective tissue of the anthers (ST3, ST4, ST6), in the placenta, the funiculus, and the outer parts of the developing seed (ST2, ST3, ST6). The observations made in this study were in agreement with the functions previously established for the three groups of M. truncatula ST proteins, as in the proposed function for ST1 in the transport and assimilation of nutrients, or the involvement of ST4, ST5, and ST6 in floral defence.

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Section: Introductionmentioning

confidence: 99%

Promoter activity of genes encoding the Specific Tissue protein family in the reproductive organs of Medicago truncatula

Albornos¹,

Martín²,

Labrador³

et al. 2019

Biol. plant.

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“…An analysis of the GmVSP α, GmVSP β, and GmN:IFR promoters using the PLACE and Plant-CARE databases revealed a number of potential cis -regulatory elements, including basal elements, such as the TATA-box, CAAT-box and TA-rich regions, as well as elements related to defense/stress, phytohormones, and growth and development. As most defense-related genes are activated more quickly and strongly in resistant interactions, elements involved in defense/stress were over-represented along the GmVSP β and GmN:IFR promoter sequences; these elements included TC-rich repeats and silencing element-binding factor (SEBF) motifs, both of which are involved in induced disease responses ( Xu et al, 2010 , 2011 ; Germain et al, 2012 ). As expected, various putative transcription factor-binding sites (TFBSs) involved in pathogen and other stresses were predicted at multiple sites in the promoter regions of GmVSP α, GmVSP β, and GmN:IFR ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of transcriptional regulatory nodes in soybean defense networks using transient co-transactivation assays

Wang

et al. 2015

Front. Plant Sci.

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Plant responses to major environmental stressors, such as insect feeding, not only occur via the functions of defense genes but also involve a series of regulatory factors. Our previous transcriptome studies proposed that, in addition to two defense-related genes, GmVSPβ and GmN:IFR, a high proportion of transcription factors (TFs) participate in the incompatible soybean-common cutworm interaction networks. However, the regulatory mechanisms and effects of these TFs on those induced defense-related genes remain unknown. In the present work, we isolated and identified 12 genes encoding MYB, WRKY, NAC, bZIP, and DREB TFs from a common cutworm-induced cDNA library of a resistant soybean line. Sequence analysis of the promoters of three co-expressed genes, including GmVSPα, GmVSPβ, and GmN:IFR, revealed the enrichment of various TF-binding sites for defense and stress responses. To further identify the regulatory nodes composed of these TFs and defense gene promoters, we performed extensive transient co-transactivation assays to directly test the transcriptional activity of the 12 TFs binding at different levels to the three co-expressed gene promoters. The results showed that all 12 TFs were able to transactivate the GmVSPβ and GmN:IFR promoters. GmbZIP110 and GmMYB75 functioned as distinct regulators of GmVSPα/β and GmN:IFR expression, respectively, while GmWRKY39 acted as a common central regulator of GmVSPα/β and GmN:IFR expression. These corresponding TFs play crucial roles in coordinated plant defense regulation, which provides valuable information for understanding the molecular mechanisms involved in insect-induced transcriptional regulation in soybean. More importantly, the identified TFs and suitable promoters can be used to engineer insect-resistant plants in molecular breeding studies.

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“…2003). Recently, the white spruce Picea glauca Defensin 1 , a homologue to Norway spruce SPI1 , was induced by methyl jasmonate (Germain et al. 2012).…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that the pathogen P. dimorphum actively suppresses expression of the putative plant defensin SPI1 in roots of Norway spruce during the later stages of infection (Sharma & Lönneborg 1996;Fossdal et al 2003). Recently, the white spruce Picea glauca Defensin 1, a homologue to Norway spruce SPI1, was induced by methyl jasmonate (Germain et al 2012). Furthermore, the P. glauca defensin promoter was found to contain motifs specific to defence and hormonal response, including the methyl jasmonate response motif, a fungal elicitor responsive element and a TC-rich repeat cis-acting element involved in defence and stress responsiveness shared with Arabidopsis thaliana defensin promoters.…”

Section: Discussionmentioning

confidence: 99%

Host responses in Norway spruce roots induced to the pathogen Ceratocystis polonica are evaded or suppressed by the ectomycorrhizal fungus Laccaria bicolor

Nagy¹,

Fossdal²

2012

Plant Biology

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The outcome of a compatible mycorrhizal interaction is different from that in a compatible plant-pathogen interaction; however, it is not clear what mechanisms are used to evade or suppress the host defence. The aim of this work is to reveal differences between the interaction of Norway spruce roots to the pathogen Ceratocystis polonica and the ectomycorrhizal Laccaria bicolor, examine if L. bicolor is able to evade inducing host defence responses typically induced by pathogens, and test if prior inoculation with the ectomycorrhizal fungus affects the outcome of a later challenge with the pathogen. The pathogen was able to invade the roots and caused extensive necrosis, leading to seedling death, with or without prior inoculation with L. bicolor. The ectomycorrhizal L. bicolor colonised primary roots of the Norway spruce seedlings by partly covering, displacing and convoluting the cells of the outer root cortex, leaving the seedlings healthy. We detected increased total peroxidase activity, and staining indicating increased lignification in roots as a response to C. polonica. In L. bicolor inoculated roots there was no increase in total peroxidase activity, but an additional highly acidic peroxidase isoform appeared that was not present in healthy roots, or in roots invaded by the pathogen. Increased protease activity was detected in roots colonised by C. polonica, but little protease activity was detected in L. bicolor inoculated roots. These results suggest that the pathogen efficiently invades the roots despite the induced host defence responses, while L. bicolor suppresses or evades inducing such host responses in this experimental system.

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The expression pattern of the Picea glauca Defensin 1 promoter is maintained in Arabidopsis thaliana, indicating the conservation of signalling pathways between angiosperms and gymnosperms*

Cited by 36 publications

References 48 publications

Promoter activity of genes encoding the Specific Tissue protein family in the reproductive organs of Medicago truncatula

Promoter activity of genes encoding the Specific Tissue protein family in the reproductive organs of Medicago truncatula

Identification of transcriptional regulatory nodes in soybean defense networks using transient co-transactivation assays

Host responses in Norway spruce roots induced to the pathogen Ceratocystis polonica are evaded or suppressed by the ectomycorrhizal fungus Laccaria bicolor

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