The Extended Granin Family: Structure, Function, and Biomedical Implications

Bartolomucci, Alessandro; Possenti, Roberta; Mahata, Sushil K.; Fischer‐Colbrie, Reiner; Loh, Y. Peng; Salton, Stephen R.J.

doi:10.1210/er.2010-0027

Cited by 291 publications

(348 citation statements)

References 417 publications

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“…In addition to those in the HPG and somatotropic axes, we also found several significant changes in factors involved in the regulation of prohormone processing, immune responses, bone metabolism, and homeostatic control in the pituitary during the juvenile-sexual maturity transition. The expression levels of Scg3 and Scg5 were significantly higher at 90 than at 45 dpf (Table 3), indicating the active modulation of prohormone modification and endoproteolytic processing in the pituitary gland of adult zebrafish (Bartolomucci et al 2011). Thymosins are important in the regulation of thymus development and in the induction of T-cell differentiation, both of which are vital for the immunological responses of organisms (Li et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…processing (Bartolomucci et al 2011). The expression levels of Scg3 and Scg5 were significantly higher at 90 dpf than at 45 dpf (Table 3).…”

Section: Rna-seq Of the Zebrafish Pituitary Transcriptomementioning

confidence: 95%

“…Moreover, highly abundant transcripts of genes involved in protein secretion or PC activity regulation, such as scg3, scg5, and pcsk1 inhibitor, like (pcsk1nl or proSAAS), were found in the pituitary tissue samples. Scg3 is a secretory protein involved in protein secretion, although no clear biological function has been found in a mammalian animal model (Bartolomucci et al 2011). Pcsk1n is a specific endogenous inhibitor of Pcsk1, which may inhibit the convertasemediated processing of Pomc and proenkephalin (Kudo et al 2009 Figure 2 Expression profiles of the ten transcripts with the highest RPKM and major pituitary hormone transcripts during the transition from the juvenile to the sexual maturity stage.…”

Section: Rna-seq Of the Zebrafish Pituitary Transcriptomementioning

confidence: 99%

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Zebrafish pituitary gene expression before and after sexual maturation

He¹,

Dai²,

Chen³

et al. 2014

Journal of Endocrinology

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Sexual maturation and somatic growth cessation are associated with adolescent development, which is precisely controlled by interconnected neuroendocrine regulatory pathways in the endogenous endocrine system. The pituitary gland is one of the key regulators of the endocrine system. By analyzing the RNA sequencing (RNA-seq) transcriptome before and after sexual maturation, in this study, we characterized the global gene expression patterns in zebrafish pituitaries at 45 and 90 days post-fertilization (dpf). A total of 15 043 annotated genes were expressed in the pituitary tissue, 3072 of which were differentially expressed with a greater than or equal to twofold change between pituitaries at 45 and 90 dpf. In the pituitary transcriptome, the most abundant transcript was gh. The expression levels of gh remained high even after sexual maturation at 90 dpf. Among the eight major pituitary hormone genes, lhb was the only gene that exhibited a significant change in its expression levels between 45 and 90 dpf. Significant changes in the pituitary transcripts included genes involved in the regulation of immune responses, bone metabolism, and hormone secretion processes during the juvenile-sexual maturity transition. Real-time quantitative PCR analysis was carried out to verify the RNA-seq transcriptome results and demonstrated that the expression patterns of the eight major pituitary hormone genes did not exhibit a significant gender difference at 90 dpf. For the first time, we report the quantitative global gene expression patterns at the juvenile and sexual maturity stages. These expression patterns may account for the dynamic neuroendocrine regulation observed in body metabolism.

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Section: Discussionmentioning

confidence: 99%

“…processing (Bartolomucci et al 2011). The expression levels of Scg3 and Scg5 were significantly higher at 90 dpf than at 45 dpf (Table 3).…”

Section: Rna-seq Of the Zebrafish Pituitary Transcriptomementioning

confidence: 95%

Section: Rna-seq Of the Zebrafish Pituitary Transcriptomementioning

confidence: 99%

See 1 more Smart Citation

Zebrafish pituitary gene expression before and after sexual maturation

He¹,

Dai²,

Chen³

et al. 2014

Journal of Endocrinology

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“…We further detected the changes of several peptides derived from other prohormones, which have been associated with important functions such as regulating metabolism and emotion (34). Two cholecystokinin-derived peptides, R2AVLRPDSE-PRAR2L and QLR2AVLRPDSEPRARLGALLARYIQQV2RVA were found to increase 34.1% (p ϭ 0.0454) and 32% (p ϭ 0.0481), respectively (Fig.…”

Section: Table I Changed Prohormone-derived Peptides and Unchanged Knmentioning

confidence: 90%

Chronic Nicotine Treatment Impacts the Regulation of Opioid and Non-opioid Peptides in the Rat Dorsal Striatum

Petruzziello

Falasca

Andrén

et al. 2013

Molecular & Cellular Proteomics

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The chronic use of nicotine, the main psychoactive ingredient of tobacco smoking, alters diverse physiological processes and consequently generates physical dependence. To understand the impact of chronic nicotine on neuropeptides, which are potential molecules associated with dependence, we conducted qualitative and quantitative neuropeptidomics on the rat dorsal striatum, an important brain region implicated in the preoccupation/ craving phase of drug dependence. We used extensive LC-FT-MS/MS analyses for neuropeptide identification and LC-FT-MS in conjunction with stable isotope addition for relative quantification. The treatment with chronic nicotine for 3 months led to moderate changes in the levels of endogenous dorsal striatum peptides. Five enkephalin opioid peptides were up-regulated, although no change was observed for dynorphin peptides. Specially, nicotine altered levels of nine non-opioid peptides derived from precursors, including somatostatin and cerebellin, which potentially modulate neurotransmitter release and energy metabolism. This broad but selective impact on the multiple peptidergic systems suggests that apart from the opioid peptides, several other peptidergic systems are involved in the preoccupation/craving phase of drug dependence. Our finding permits future evaluation of the neurochemical circuits modulated by chronic nicotine exposure and provides a number of novel molecules that could serve as potential therapeutic targets for treating drug dependence. Molecular & Cellular

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“…from secretory granules of chromaffin cells [1,2]. CHGA plays a major role in the biogenesis of catecholamine storage vesicles [3,4].…”

mentioning

confidence: 99%