The Extraction and Activity Test of Bioactive Compounds in Phaleria Macrocarpa as Antioxidants

Andrean, David; Prasetyo, Susiana; Kristijarti, Anastasia Prima; Hudaya, Tedi

doi:10.1016/j.proche.2014.05.012

Cited by 17 publications

(8 citation statements)

References 7 publications

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“…Previous studies on the chemical composition of the pericarp and pericarpof PM found alkaloids, saponins, flavonoids, and polyphenols. (21)(22)(23) The decrease in blood glucose is effected by the action of saponins and flavonoids. The effect of saponin on the cell membrane inhibits the absorption of the smaller nutrient molecules that should be absorbed, such as glucose, Abnormalities in cell structure are thought to cause disturbances in the glucose transporter system, leading to inhibition of glucose absorption.…”

Section: Discussionmentioning

confidence: 99%

Hypoglycemic effect of Phaleria macrocarpa (Scheff.) Boerl dry extract in healthy adults

et al. 2018

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Background The prevalence of diabetes in Indonesia continues to increase and causes many complications. Fruits of Phaleria macrocarpa (Scheff.) Boerl. (PM) are used in the traditional health system of the Indonesians, as effective remedy in the management of diabetes mellitus. The aim of this study was to evaluate the effect and magnitude of doses of PM dry fruit extract (PME) on blood glucose levels in healthy adults. Methods This research was of pre-posttest design involving 30 healthy volunteer subjects aged 20-55 years receiving glucose loads. At pre-test the subjects were given orally 75 g glucose, and at post-test they were given PME at doses of 125 mg and 250 mg. Data collection included anthropometric examination and blood glucose level. The area under the curve (AUC) of blood glucose levels was measured at 0, 15, 30, 60, 120 and 180 minutes. Data analysis was performed using paired t-test with p <0.05. Results The percentage of reduction in AUC between pre- and post-test after administration of PME 125 mg was 12.1% and significant (p=0.000). But the percentage of reduction in AUC between pre- and post-test after administration of PME 250 mg was 4.07% and not significant (p=0.06). The reduction in AUC between post-test PME 125 mg vs PME 250 mg showed significant results (p=0.011). Conclusion This study demonstrated that a dose of 125 mg Phaleria macrocarpa (Scheff.) Boerl. dry extract has a hypoglycemic effect in healthy adults, and may therefore be suitable for use as traditional anti-diabetic drug.

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Section: Discussionmentioning

confidence: 99%

Hypoglycemic effect of Phaleria macrocarpa (Scheff.) Boerl dry extract in healthy adults

et al. 2018

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“…Phaleria macrocarpa fruit is a traditional herb found in some Asia regions, especially Indonesia. Phaleria macrocarpa was traditionally used for reducing cholesterol, atherosclerosis, and diabetes mellitus [14][15][16][17]. Our previous study revealed that Phaleria macrocarpa contained six flavonoids, including eriodictyol, glycitin, 5-O-methylgenistein, catechin 7-O-beta-D-xyloside, 8prenylnaringenin, and naringenin and inhibited peritoneal damage in the endometriosis mice model [18].…”

Section: Introductionmentioning

confidence: 99%

Phaleria macrocarpa Flavonoid as a Potent MMP-1 Inhibitor for Endometriosis Therapy: In silico Study

Maharani

Sutrisno

2022

a-jhr

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Introduction: Endometriosis is a gynaecological disorder in women and causes infertility. Several therapeutic were developed to reduce endometriosis cases, one of them was matrix metalloproteinase (MMP) inhibitor. This study investigated the potential activity of the flavonoids in Phaleria macrocarpa fruit as MMP1 inhibitors for endometriosis. Methods: In silico modelling was used in this study. Six flavonoid structures were retrieved from PubChem NCBI database. A targeted protein, MMP1 was taken out from Protein Data Bank with ID 1CGE and predicted the active sites. Six flavonoids and MMP1 was interacted by molecular docking using Molegro virtual Docker version 5 and analyzed using PyMol 2.2 and Discovery Studio version 21.1.1. Three-dimensional complex structure of flavonoids – MMP1 showed interaction in the same active sites and performed an amino acid residue Glu219 as catalytic site. Results: Six flavonoids of Phaleria macrocarpa were divided into two patterns and generated varied binding energy. Glycitin and Catechin 7-O-beta-D-xyloside showed low score of binding energy and depicted similar structure with four aromatic rings. 8-Prenylnaringenin performed lower binding energy than naringenin, eriodictyol, and 5-O-methylgenistein. Conclusion: In silico analysis suggested that six flavonoids compounds is potent as MMP1 inhibitor and might be interfered endometriosis pathophysiology. In vivo and in vivo investigations are required for further analysis.

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“…Previous studies investigated the phytochemicals of P. macrocarpa leaf and fruit, which showed the existence of bioactive compounds such as flavonoids, polyphenols, saponins, tannins, and lignins. These compounds have medicinal values and may be attributed to the high chemopreventive and antioxidant activities comparable to modern medicines (Alara et al., 2017; Andrean et al., 2014; Lim et al., 2019; Shwter et al, 2016). Several studies have reported that P. macrocarpa leaf and fruit have multiple beneficial pharmacological activities, such as antimicrobials (Hendra et al., 2011), antioxidants (Lay, Karsani, Banisalam et al, 2014), anti‐cancers (Rahmawati et al., 2006), anti‐inflammatory (Tjandrawinata et al., 2011; Wiweko et al., 2015), antiproliferative (Wiweko et al, 2015; Tjandrawinata et al., 2011), sexual enhancement (Parhizkar et al., 2013), and may significantly reduce body weight gain and total cholesterol (Chong et al., 2011).…”

Section: Introductionmentioning

confidence: 99%

Extraction and identification of biologically important proteins from the medicinal plant God’s crown ( Phaleria macrocarpa )

Habib

Ismail

2021

J Food Biochem

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The fruit and leaf of God’s crown (Phaleria macrocarpa) have been traditionally used to treat a wide variety of diseases. However, the proteins of this tropical plant are still heavily understudied. Three protein extraction methods; phenol (Phe), trichloroacetic acid (TCA)–acetone–phenol (TCA–A–Phe), and ultrasonic (Ult) were compared on the fruit and leaf of P. macrocarpa. The Phe extraction method showed the highest percentage of recovered protein after the resolubilization process for both leaf (12.24%) and fruit (30.41%) based on protein yields of the leaf (6.15 mg/g) and fruit (36.98 mg/g). Phe and TCA–A–Phe extraction methods gave well‐resolved bands over a wide range of molecular weights through sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Following liquid chromatography‐tandem mass spectrometry analysis, proteins identified through the Phe extraction method were 30%–35% enzymatic proteins, including oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases that possess various biological functions. Practical applications Every part of God’s crown plant is traditionally consumed to treat various illnesses. While plant’s benefits are well known and have led to a plethora of health products, the proteome remains mostly unknown. This study compares three protein extraction methods for the leaf and fruit of P. macrocarpa and identifies their proteins thru LC‐MS/MS coupled with PEAKS. These method comparisons can be a guide for works on other plants as well. In addition, the proteomics data from this study may shed light on the functional properties of these plant parts and their products.

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The Extraction and Activity Test of Bioactive Compounds in Phaleria Macrocarpa as Antioxidants

Cited by 17 publications

References 7 publications

Hypoglycemic effect of Phaleria macrocarpa (Scheff.) Boerl dry extract in healthy adults

Hypoglycemic effect of Phaleria macrocarpa (Scheff.) Boerl dry extract in healthy adults

Phaleria macrocarpa Flavonoid as a Potent MMP-1 Inhibitor for Endometriosis Therapy: In silico Study

Extraction and identification of biologically important proteins from the medicinal plant God’s crown ( Phaleria macrocarpa )

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