The Extracts from Allium hookeri induces p53-independent Apoptosis through Mitochondrial Intrinsic Pathways in AGS Human Gastric Carcinoma Cells

Nam, Gun He; Lee, Se Il; Kim, Ga Yeon; Jeon, Mi Ji; Jo, Kyung Jo; Park, Ye Seul; Kim, Sang Yong; Kim, Young Min

doi:10.4172/1948-5956.1000544

Cited by 3 publications

(1 citation statement)

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“…Recently, many cancer-related studies have been conducted on traditional medicinal plants, with the focus of identifying new compounds with physiological effects and investigating their potential value as anticancer drugs (30). These studies provide the basis for developing anticancer drugs that have fewer side effects and that can serve as an alternative to mitigate commercial anticancer drug resistance (31,32). Since the characteristics of many cancer cells, such as invasiveness and metastasis, are mediated by anoikis resistance, targeting and treating cancer cells are often difficult.…”

Section: Discussionmentioning

confidence: 99%

Scaphium affine Ethanol Extract Induces Anoikis by Regulating the EGFR/Akt Pathway in HCT116 Colorectal Cancer Cells

et al. 2021

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Scaphium affine ethanol extracts (SAE) is a species that has been shown to contain various physiological effects; however, its anticancer effects have yet to be revealed. We qualitatively evaluated β-sitosterol in SAE through high-performance liquid chromatography (HPLC). The cytotoxicity in HCT116 and HT29 colorectal cancer cells and CCD841 normal colon cells was confirmed through WST-1 assays. Selective cytotoxicity was observed in colorectal cancer cells, with greater cytotoxicity demonstrated in the HCT116 cell line. As such, the HCT116 colorectal cell line was selected for subsequent experiments. After HCT116 cells were treated with SAE, it was confirmed that the apoptosis rate was increased in a SAE dose-dependent manner through Annexin V assay. SAE further showed dose-dependent suppression of invasion through invasion assays. Anoikis induction through the EGFR/Akt pathway in HCT116 colorectal cancer cells was confirmed by Western blotting. The tumor suppressive effects of SAE was assessed in vivo using a xenograft model of human HCT116 colorectal cancer cells. As a result, we confirmed that SAE decreased tumor size in a dose-dependent manner and that p-EGFR and cleaved-caspase 3 in tumors were also regulated in a dose-dependent manner. This study showed that SAE, by containing β-sitosterol with proven anticancer effects, induces anoikis through the EGFR/Akt pathway in HCT116 colorectal cancer cells both in vitro and in vivo.

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Section: Discussionmentioning

confidence: 99%

Scaphium affine Ethanol Extract Induces Anoikis by Regulating the EGFR/Akt Pathway in HCT116 Colorectal Cancer Cells

et al. 2021

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In vitro and in vivo Induction of p53-Dependent Apoptosis by Extract of Euryale ferox Salisb in A549 Human Caucasian Lung Carcinoma Cancer Cells Is Mediated Through Akt Signaling Pathway

Nam

Park

et al. 2019

Front. Oncol.

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Lung cancer is one of the leading causes of death, and mortality rates have steadily been increasing. Recently, several studies were conducted to develop novel, physiologically active compounds from medicinal plant extracts. Several plant-derived extracts and molecules regulate and inhibit signaling molecules associated with the growth and proliferation of cancer cells. Euryale ferox salisb is a medicinal plant that is effective against different types of cancers. In this study, we investigated the apoptotic effects of E. ferox salisb extract (ESE) in A549 lung cancer cells, exerted by the inhibition of the Akt protein and activation of the p53 protein. Our results show that ESE induces apoptosis via the regulation of mitochondrial outer membrane potential and generation of reactive oxygen species (ROS). We demonstrate that apoptosis is induced in a p53-dependent manner when cells are treated with pifithrin-α (a p53 inhibitor) and LY294002 (an Akt inhibitor). The apoptotic effects from ESE were observed in vivo in Balb/c-nu mice bearing A549 xenografts. Altogether, these results suggest that E. ferox salisb extracts exert anti-cancer effects in a p53-dependent manner.

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Apoptotic Effects of Butanol Fraction of Lysimachia foenum-graecum by Regulating Akt/mTOR Signaling Pathways in AGS Gastric Cancer Cells

Lee

Seo

Kim

et al. 2022

JKFN

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The Extracts from Allium hookeri induces p53-independent Apoptosis through Mitochondrial Intrinsic Pathways in AGS Human Gastric Carcinoma Cells

Cited by 3 publications

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Scaphium affine Ethanol Extract Induces Anoikis by Regulating the EGFR/Akt Pathway in HCT116 Colorectal Cancer Cells

Scaphium affine Ethanol Extract Induces Anoikis by Regulating the EGFR/Akt Pathway in HCT116 Colorectal Cancer Cells

In vitro and in vivo Induction of p53-Dependent Apoptosis by Extract of Euryale ferox Salisb in A549 Human Caucasian Lung Carcinoma Cancer Cells Is Mediated Through Akt Signaling Pathway

Apoptotic Effects of Butanol Fraction of Lysimachia foenum-graecum by Regulating Akt/mTOR Signaling Pathways in AGS Gastric Cancer Cells

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