The extracts from Nelumbo Nucifera suppress cell cycle progression, cytokine genes expression, and cell proliferation in human peripheral blood mononuclear cells

Liu, Chih-Peng; Tsai, Wei‐Jern; Lin, Yuang-Lian; Liao, Junyi; Chen, Chieh-Fu; Kuo, Yur‐Ren

doi:10.1016/j.lfs.2004.01.019

Cited by 156 publications

(117 citation statements)

References 29 publications

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“…In one study, an inverse correlation was observed between the consumption of certain medicinal herbs, including sage and rosemary, and the incidence of lung cancer (5). In addition, traditional Chinese herbs have the characteristic of suppressing the expression of several genes involved in cancer (6). The inhibition of AKT expression is expected to interfere with the progression of cancer.…”

Section: Introductionmentioning

confidence: 99%

Terpinolene, a component of herbal sage, downregulates AKT1 expression in K562 cells

et al. 2011

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Abstract. Protein kinase AKT mediates cell proliferation and survival signals, and also contributes to cancer progression. Increased expression and/or activation of AKT is involved in a variety of human cancers. In cells treated with sage or rosemary extract, mRNA and protein expression levels of AKT1 were reduced compared with those of the control cells 48 h after the herbal treatments. We found that terpinolene, a common component of sage and rosemary, markedly reduced the protein expression of AKT1 in K562 cells and inhibited cell proliferation.

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Section: Introductionmentioning

confidence: 99%

Terpinolene, a component of herbal sage, downregulates AKT1 expression in K562 cells

et al. 2011

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“…The seed of Nelumbo nucifera (Gaertn), also known as the lotus, is traditionally used in Chinese folk medicine. A number of previous studies have reported that the lotus seed exhibits numerous health benefits and pharmacological effects, such as anti-ischemic (11), antioxidant (12)(13)(14), hepatoprotective (12), antiproliferative (15)(16)(17)(18)(19), anti-inflammatory (20), anti-infertility (21), anti-arrhythmic (22)(23)(24)(25)(26), antifibrotic (27) and antiviral (28) activities. In the present study, the anticancer activity of alkaloids extracted from the Ba lotus seed (BLSA), a new variety of Nelumbo nucifera, which only grows in Chongqing, a city located in the southwest of China, was investigated in human NPC CNE-1 cells.…”

Section: Anticancer Activities Of Alkaloids Extracted From the Ba Lotmentioning

confidence: 99%

Anticancer activities of alkaloids extracted from the Ba lotus seed in human nasopharyngeal carcinoma CNE-1 cells

Zhao

Xia

Peng³

et al. 2016

Experimental and Therapeutic Medicine

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Abstract.To investigate the anticancer activities of alkaloids from the Ba lotus seed (BLSA) in human nasopharyngeal carcinoma (NPC) CNE-1 cells, an MTT assay, flow cytometry, reverse transcription-polymerase chain reaction and western blotting were performed. BLSA was found to significantly reduce CNE-1 cell proliferation in a dose-dependent manner at all concentrations compared with the control (P<0.05). In addition, flow cytometry analysis identified that BLSA treatment significantly increased the sub-G1 content in CNE-1 cells (P<0.05). Following BLSA treatment, the mRNA and protein levels of a number of apoptosis-related factors, such as caspase family members (caspase-3, -8 and -9), B-cell lymphoma (Bcl)-2-associated X protein, Fas and Fas ligand were significantly increased compared with the control (P<0.05). This was accompanied by a significant decrease in anti-apoptotic Bcl-2 and Bcl-extra large protein expression compared with the control (P<0.05). Furthermore, BLSA treatment was determined to modulate CNE-1 cell expression of nuclear factor (NF)-κB and NF-κB inhibitor α. The results of the present study indicate that BLSA has anticancer activity through inducing cellular apoptosis. In addition, these results suggest that BLSA can be used as a therapeutic agent in NPC.

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“…Traditional herbal medications are commonly used to treat colon cancer in the clinic (11)(12)(13). Lotus is traditionally used as a Chinese folk medicine to disperse the summer heat, and has shown numerous health benefits and pharmacological activities, including antioxidant (14), antidiarrheal (15), antiviral (16), anti-obesity (17)(18)(19)(20), anti-angiogenic (21), hepatoprotective (22), immunomodulatory (23) and insulin secretagogue activity (24). Therefore, the present study focused on investigating the potential anticancer activity of Nelumbo nucifera (Ba lotus) stamen ethanol crude extract (BLSEE), and also to elucidate the mechanisms underlying its anticancer effects in human colon carcinoma HCT-116 cells.…”

Section: Introductionmentioning

confidence: 99%

Anticancer activity of Nelumbo nucifera stamen extract in human colon cancer HCT-116 cells in vitro

et al. 2016

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Abstract. The aim of the present study was to investigate the anticancer activities of Nelumbo nucifera (Ba lotus) stamen ethanol crude extract (BLSEE) in human colon carcinoma HCT-116 cells. MTT assay, flow cytometry analysis and reverse transcription-polymerase chain reaction assay were employed to investigate the anticancer mechanisms of BLSEE (100, 200 and 400 µg/ml) in HCT-116 cells. BLSEE reduced HCT-116 cell proliferation in a dose-dependent manner. BLSEE treatment also significantly increased the sub-G1 population in HCT-116 cells (P=0.0020 at 400 µg/ml), as shown by flow cytometry assay. Following treatment with BLSEE, the mRNA levels of the apoptosis-associated factors Fas, Fas ligand, tumor necrosis factor-related apoptosis-inducing ligand, death receptor 4 (DR4), death receptor 5 (DR5), caspases 3, 8 and 9, and B-cell lymphoma-2 (Bcl-2) associated X protein were increased, and the expression of anti-apoptotic Bcl-2 and Bcl-extra large was decreased in HCT-116 cells. The mRNA levels of matrix metalloproteinase (MMP)-2, MMP-9, TIMP metallopeptidase inhibitor 1 and TIMP metallopeptidase inhibitor 2 were also regulated by BLSEE treatment. In addition, BLSEE was able to modulate the expression of inflammation-associated nuclear factor-κB, inhibitory κBα, inducible nitric oxide synthase and cyclooxygenase 2 in HCT-116 cells. The present study clearly indicated the cytotoxicity of BLSEE in HCT-116 cells through induced cellular apoptosis. These results also suggested the BLSEE may be a powerful agent against colon cancer cells. IntroductionColorectal cancer (CRC) is one of the most frequently diagnosed cancers in the world. In the USA, CRC is the third most common malignant tumor, and there was an estimated 103,170 new cases of CRC throughout the country in 2014 (1). Previously, CRC has become the fourth most common malignant tumor, and the third leading cause of cancer-associated mortality in China (2). CRC also shows a high level of metastasis, and 25% of CRC patients that present with metastatic disease have a 5-year survival of only 10% (3). Surgical resection plus chemotherapy and/or radiation therapy are effective treatments for CRC in the clinic. However, chemotherapy and radiation therapy are commonly associated with serious side effects, including bone marrow suppression, nausea and vomiting, hair loss and loss of appetite (3). Enhancement of cancer cell apoptosis is a good strategy in the clinical treatment of cancer (4).As classic apoptosis-associated factors, the B-cell lymphoma 2 (Bcl-2) family plays an important role in the regulation of cell apoptosis. The Bcl-2 family is generally divided into anti-apoptotic factors, including Bcl-2, Bcl-extra large (Bcl-xL) and Bcl-W, and proapoptotic factors, including Bcl-2-associated X protein (Bax), Bcl-2 associated agonist of cell death, Bcl-2 interacting mediator of cell death (Bim), Bcl-2 antagonist/killer 1 and p53 upregulated modulator of apoptosis (5,6). In the extrinsic apoptosis pathway, the cell death receptor Fas/Fas ligand (FasL) system plays...

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The extracts from Nelumbo Nucifera suppress cell cycle progression, cytokine genes expression, and cell proliferation in human peripheral blood mononuclear cells

Cited by 156 publications

References 29 publications

Terpinolene, a component of herbal sage, downregulates AKT1 expression in K562 cells

Terpinolene, a component of herbal sage, downregulates AKT1 expression in K562 cells

Anticancer activities of alkaloids extracted from the Ba lotus seed in human nasopharyngeal carcinoma CNE-1 cells

Anticancer activity of Nelumbo nucifera stamen extract in human colon cancer HCT-116 cells in vitro

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