The Filamentous Bacterial Morphotype ‘Nostocoida limicola’ I Contains at least Two Previously Described Genera in the Low G+C Gram Positive Bacteria

Liu, Jian Rong; Burrell, P. C.; Seviour, Elizabeth M.; Soddell, Jacques A.; Blackall, Linda L.; Seviour, Robert J.

doi:10.1016/s0723-2020(00)80027-2

Cited by 30 publications

(30 citation statements)

References 19 publications

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“…Interestingly, 5 of these phylotypes clustered particularly closely with those from Lake Baikal . The most closely related bacteria are Cellulomonas sp., Geodermatophilus sp., and 'Nostocoida limicola', of which the latter occurs in activated sludge with only a few known strains (Liu et al 2000). The other 7 phylotypes of the high GC Actinobacteria are related to the deep-branching cluster of 'Microthrix parvicella'.…”

Section: Discussionmentioning

confidence: 99%

Seasonal patterns of the bacterioplankton community composition in a large mesotrophic lake

Zwisler¹,

Selje²,

Simon³

2003

Aquat. Microb. Ecol.

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The composition of the bacterioplankton community in mesotrophic Lake Constance was studied from April 1996 to December 1997 by fluorescence in situ hybridization (FISH) with group specific oligonucleotide probes and denaturing gradient gel electrophoresis (DGGE). Between 10 and 59% of the DAPI-stained cells were detected as Bacteria with means of 38 ± 12, 27 ± 10 and 23 ± 6% at 3, 20, and 50 m depth, respectively. In the euphotic zone at 3 m depth, β-Proteobacteria always constituted highest proportions of the bacterial community detected by FISH and constituted 6 to 23% of the DAPIstained cells with a mean of 12%. Proportions of bacteria belonging to the Cytophaga/Flavobacteria (CF) cluster and the α-Proteobacteria at this depth were 5.4 ± 2.5 and 4.1 ± 3.5%, respectively. Proportions of β-Proteobacteria decreased at 20 and 50 m, whereas that of α-Proteobacteria remained constant and that of the CF-cluster increased to 7.4%, such that they comprised similar proportions as β-Proteobacteria at 50 m depth. γ-Proteobacteria were always of minor importance and constituted only 2.2 ± 1.3% of the DAPI cell counts. Cumulative proportions of all detected bacterial groups constituted 75% of the Bacteria counts at 3 m, 84% at 20 m and 99% at 50 m. The DGGE analysis of the bacterioplankton community showed seasonal and vertical variations of the banding patterns even though several bands occurred throughout the study period and at all depths. A cluster analysis revealed distinct bacterial clusters of the phytoplankton spring bloom and the later period and of epilimnetic and hypolimnetic waters. The sequence analysis of the excised bands indicated that all groups which were detected by FISH were represented in the patterns. Sequence similarities of the retrieved sequences to known isolates were < 90 to 96.1%, and to other phylotypes as close as 99%. Phylotypes of β-Proteobacteria and Actinobacteria of the typical banding patterns affiliated closely with recently described clusters of typical freshwater bacteria. In addition, we identified phylotypes of the Holophaga/Acidobacterium and Nitrospira phylum during the stratified period in samples from 50 m and during mixing from all 3 depths. KEY WORDS: Bacteria · DGGE · Fluorescence in situ hybridization · Lake Constance · Actinobacteria · Nitrospira · Holophaga Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 31: [211][212][213][214][215][216][217][218][219][220][221][222][223][224][225] 2003

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Section: Discussionmentioning

confidence: 99%

Seasonal patterns of the bacterioplankton community composition in a large mesotrophic lake

Zwisler¹,

Selje²,

Simon³

2003

Aquat. Microb. Ecol.

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“…The peptidoglycan type of the strains was determined according to the protocol of Schumann et al (1997). Cells were prepared and viewed under a scanning electron microscope, using the methods described by Liu et al (2000).…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, 16S rRNA sequence analysis has revealed that T. flocculiformis Echt T is almost identical to Lactosphaera pasteurii KoTa2 T (Stackebrandt et al, 1999), a nonfilamentous, aerotolerant, lactate-producing coccus belonging to the low-GjC Gram-positive bacteria, originally isolated from anoxic digester sludge (Janssen et al, 1995). Liu et al (2000) showed that, on the basis of similarities in their 16S rRNA sequences, some isolates of a bulking filamentous bacterium they described as ' Nostocoida limicola ' I (van Veen, 1973 ;…”

Section: Introductionmentioning

confidence: 99%

Emended description of the genus Trichococcus, description of Trichococcus collinsii sp. nov., and reclassification of Lactosphaera pasteurii as Trichococcus pasteurii comb. nov. and of Ruminococcus palustris as Trichococcus palustris comb. nov. in the low-G+C Gram-positive bacteria

Liu¹

2002

International Journal of Systematic and Evolutionary Microbiology

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Analyses of 165 rRNA gene sequences, restriction endonuclease digestion fingerprints of 16S-23S intergenic regions, DNA base compositions, fatty-acid profiles, cell-wall chemistry, cell physiology and fermentation end-product composition, along with other biochemical and phenotypic properties, supported the view that Trichococcus flocculiformis EchtT (DSM 2094T), Lactosphaera pasteurii KoTa2T (DSM 2381T), Ruminococcus palustris Z-7189T (DSM 9172T) and an isolate named 'Carnococcus allantoicus' NDP were all very similar and should be merged into a single genus. Detailed characterization of strains Ben 77, Ben 200 and Ben 201 described previously as 'Nostocoida limicola' I, a filamentous bacterium which causes bulking in activated sludge systems, revealed that these strains also belonged to the same genus as T. flocculiformis EchtT, L. pasteurii KoTa2T, R. palustris Z-7189T and 'C allantoicus' NDP. In fact, their shared properties suggested that these strains all belonged to a single species. However, DNA-DNA hybridization data indicated that T. flocculiformis EchtT, all of the 'N. limicola' I isolates and 'C allantoicus' NDP belonged to the same species, whereas L. pasteurii KoTa2T, R. palustris Z-7189T and two new isolates, 37AN3*T and 45AN2, represented three distinct species within the same genus. The priority of the genus name Trichococcus is established and since its validation predates the description of the genus Lactosphaera this name should take precedence. Under certain culture conditions, all of the strains mentioned above could produce chains of cocci. Furthermore, the morphology of T. flocculiformis EchtT could change to a non-filamentous form on certain media. This study proposes that the above strains be reclassified as members of the genus Trichococcus as four species, namely Trichococcus flocculiformis emend. (type strain EchtT = DSM 2094T), Trichococcus pasteurii comb. nov. (type strain KoTa2T = DSM 2381T = ATCC 35945T), Trichococcus collinsii sp. nov. (type strain 37AN3*T = DSM 14526T = ATCC BAA-296T, and Trichococcus palustris comb. nov. (type strain Z-7189T = DSM 9172T).

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“…3). Liu et al showed Trichococcus Nostocoida limicola I to be a type of filamentous bacteria in AS samples (Liu et al, 2000), but it was not detected by the BFB database. Therefore, we considered that other novel filamentous bacteria of Trichococcus might exist in AS samples in the investigated bulking WWTP.…”

Section: Biological and Environmental Factors Causing Sludge Bulkingmentioning

confidence: 99%

“…Other filamentous members, such as Eikelboom morphotype 0803 of Chloroflexi, have also been found in AS WWTPs, and they have occasionally been associated with bulking incidences (Beer et al, 2002;Bj€ ornsson et al, 2002;Speirs et al, 2015). Moreover, the filamentous species of Bacteroidetes such as Haliscomenobacter hydrossis and Firmicutes such as Trichococcus Nostocoida limicola I have been isolated from AS (Nielsen et al, 2009;Liu et al, 2000;Muszynski, 2016), and they may have a slight effect on biomass settling properties . In fact, different types of filamentous bacteria may exist in WWTPs with or without obvious sludge bulking.…”

Section: Introductionmentioning

confidence: 99%

Detailed comparison of bacterial communities during seasonal sludge bulking in a municipal wastewater treatment plant

Wang

et al. 2016

Water Research

104

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a b s t r a c tIn this study, pyrosequencing combined with clone library analysis, qPCR, and fluorescent in situ hybridization (FISH) were performed to identify detailed changes of bacterial and filamentous bacterial communities in activated sludge (AS) in 3 types of typical AS samples: sludge bulking (B-AS), excessive bulking (EB-AS), and non-bulking (N-AS). Sludge bulking resulted in a decrease in total bacterial numbers from (6.4 ± 0.18) Â 10 8 gene copies/mL in N-AS to (2.4 ± 0.22) Â 10 8 in EB-AS and a decrease in bacterial diversity from 2757 OTUs in N-AS to 2217 OTUs in EB-AS. With the occurrence of sludge bulking, Actinobacteria and Firmicutes increased sharply, whereas Proteobacteria, which was the predominant phylum in N-AS, decreased markedly. In addition, Nitrospirae, a major lineage of the nitrite-oxidizing bacteria, had quite a low abundance in EB-AS (0.15%), while it was relatively high in N-AS (1.17%). On the other hand, filamentous bacteria accounted for 28.77% and 5.72% of total sequences in EB-AS and N-AS, respectively. More interestingly, 11 types of filamentous bacteria were always present in 3 types of typical AS samples from different stages of sludge bulking, and most of them enriched in EB-AS compared to N-AS. It is noteworthy that, in addition to the frequently reported filamentous bacteria such as Candidatus M. parvicella and Tetrasphaera, novel filamentous species of Trichococcus might exist in this bulking WWTP. Our results reveal that sludge bulking are derived from diverse taxa, which expands previous understanding and provides new insight into the underlying complications of the bulking phenomenon in AS.

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The Filamentous Bacterial Morphotype ‘Nostocoida limicola’ I Contains at least Two Previously Described Genera in the Low G+C Gram Positive Bacteria

Cited by 30 publications

References 19 publications

Seasonal patterns of the bacterioplankton community composition in a large mesotrophic lake

Seasonal patterns of the bacterioplankton community composition in a large mesotrophic lake

Emended description of the genus Trichococcus, description of Trichococcus collinsii sp. nov., and reclassification of Lactosphaera pasteurii as Trichococcus pasteurii comb. nov. and of Ruminococcus palustris as Trichococcus palustris comb. nov. in the low-G+C Gram-positive bacteria

Detailed comparison of bacterial communities during seasonal sludge bulking in a municipal wastewater treatment plant

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