The first occurrence of stem canker on oilseed rape caused by Leptosphaeria biglobosa in Serbia

Mitrović, Petar; Marjanović-Jeromela, Ana; Тркуља, Војислав; Milovac, Željko; Terzić, S.

doi:10.5937/ratpov53-8997

Cited by 4 publications

(1 citation statement)

References 31 publications

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“…To a great extent spore germination and mycelial growth [54] and hence, also the stem canker disease is controlled by plant resistance and fungicide application [55], especially when the latter are well-timed by the decision support systems However, naturally created mechanisms such as fast growth, abundant reproduction, and higher levels of resistance to commonly used fungicides are in favor of the survival of P. biglobosus. It was usually found as the first species on oilseed rape [1,33,37,46], and in some countries, out of the two species, only P. biglobosus has been reported on oilseed rape by now [57].…”

Section: Discussionmentioning

confidence: 99%

Metabolic Capacity Differentiates Plenodomus lingam from P. biglobosus Subclade ‘brassicae’, the Causal Agents of Phoma Leaf Spotting and Stem Canker of Oilseed Rape (Brassica napus) in Agricultural Ecosystems

2022

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In contrast to the long-lasting taxonomic classification of Plenodomus lingam and P. biglobosus as one species, formerly termed Leptosphaeria maculans, both species form separate monophyletic groups, comprising sub-classes, differing considerably with epidemiology towards Brassicaceae plants. Considering the great differences between P. lingam and P. biglobosus, we hypothesized their metabolic capacities vary to a great extent. The experiment was done using the FF microplates (Biolog Inc., Hayward, CA, USA) containing 95 carbon sources and tetrazolium dye. The fungi P. lingam and P. biglobosus subclade ‘brassicae’ (3 isolates per group) were cultured on PDA medium for 6 weeks at 20 °C and then fungal spores were used as inoculum of microplates. The test was carried out in triplicate. We have demonstrated that substrate richness, calculated as the number of utilized substrates (measured at λ490 nm), and the number of substrates allowing effective growth of the isolates (λ750 nm), showed significant differences among tested species. The most efficient isolate of P. lingam utilized 36 carbon sources, whereas P. biglobosus utilized 60 substrates. Among them, 25–29 carbon sources for P. lingam and 34–48 substrates for P. biglobosus were efficiently used, allowing their growth. Cluster analysis based on Senath criteria divided P. biglobosus into two groups and P. lingam isolates formed one group (33% similarity). We deduce the similarities between the tested species help them coexist on the same host plant and the differences greatly contribute to their different lifestyles, with P. biglobosus being less specialized and P. lingam coevolving more strictly with the host plant.

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Section: Discussionmentioning

confidence: 99%

Metabolic Capacity Differentiates Plenodomus lingam from P. biglobosus Subclade ‘brassicae’, the Causal Agents of Phoma Leaf Spotting and Stem Canker of Oilseed Rape (Brassica napus) in Agricultural Ecosystems

2022

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Phylogenetic analysis of Plenodomus lingam and Plenodomus biglobosus isolates in Hungary

2023

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Blackleg (stem canker) of crucifers is a globally important disease caused by multiple genetic subclades of the fungi Plenodomuslingam (syn.: Leptosphaeria maculans) and Plenodomus biglobosus (syn.: Leptosphaeria biglobosa). In our study, we monitored the geographical distribution of these two pathogen species from rapeseed growing areas in Hungary. Multiplex PCR identified 48.7% of the isolates as Plenodomus biglobosus, which indicates the non-recent introduction of the pathogen into Hungary. In addition, multi-locus analysis revealed low genetic diversity within the species, as all isolates were clustered to the Plenodomuslingam ‘brassicae’ and Plenodomus biglobosus ‘brassicae’ subclades. The low genetic diversity of a population generally means reduced adaptation potential, which is essential information in breeding and in developing more effective management strategies.

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Leptosphaeria biglobosa

2021

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The first occurrence of stem canker on oilseed rape caused by Leptosphaeria biglobosa in Serbia

Cited by 4 publications

References 31 publications

Metabolic Capacity Differentiates Plenodomus lingam from P. biglobosus Subclade ‘brassicae’, the Causal Agents of Phoma Leaf Spotting and Stem Canker of Oilseed Rape (Brassica napus) in Agricultural Ecosystems

Metabolic Capacity Differentiates Plenodomus lingam from P. biglobosus Subclade ‘brassicae’, the Causal Agents of Phoma Leaf Spotting and Stem Canker of Oilseed Rape (Brassica napus) in Agricultural Ecosystems

Phylogenetic analysis of Plenodomus lingam and Plenodomus biglobosus isolates in Hungary

Leptosphaeria biglobosa

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