The first trimester human trophoblast cell line ACH-3P: A novel tool to study autocrine/paracrine regulatory loops of human trophoblast subpopulations – TNF-α stimulates MMP15 expression

Abstract

Background: The trophoblast compartment of the placenta comprises various subpopulations with distinct functions. They interact among each other by secreted signals thus forming autocrine or paracrine regulatory loops. We established a first trimester trophoblast cell line (ACH-3P) by fusion of primary human first trimester trophoblasts (week 12 of gestation) with a human choriocarcinoma cell line (AC1-1).

Decidualized endometrial stromal cell derived factors promote trophoblast invasion

“…Both the cell lines have been extensively characterized for their resemblance to primary trophoblast cells (15,16). The JEG-3 cells are invasive choriocarcinoma cells; ACH-3P is a hybrid of primary trophoblasts and a choriocarcinoma cell line containing a mixed population of trophoblast cells including invading EVTs (16).…”

Toll-like receptor profiling of seven trophoblast cell lines warrants caution for translation to primary trophoblasts

“…The cell lines AC1M-32 [19] and ACH-3P [20] have been generated by fusion of the AC1-1 cell line (a JEG-3 mutant [21]) with primary term or first trimester trophoblasts, respectively. In addition, trophoblast cell lines such as HTR-8/SVneo [22] and SGHPL-5 [23] have been generated by SV40 large T antigen transfection.…”

Human Leukocyte Antigen-G (HLA-G) Expression in Cancers: Roles in Immune Evasion, Metastasis and Target for Therapy

“…Indeed, the relationship between HLA-G and MMP expression was addressed in previous studies. Hiden et al (108) addressed that MMP-15 expression could be induced by TNF-α only in the HLA-G positive first trimester human trophoblast cell line ACH-3P, and only the HLA-G positive extravillous trophoblasts (EVT) have the invasive potential. Much higher MMP-14 expression was also found in HLA-G positive first-trimester invasive trophoblasts (109).…”