The first World Cell Race

Maiuri, Paolo; Terriac, Emmanuel; Paul‐Gilloteaux, Perrine; Vignaud, Timothée; McNally, Krista; Onuffer, James; Thorn, Kurt S.; Nguyen, Phuong; Georgoulia, Nefeli; Soong, Daniel; Jayo, Asier; Beil, Nina; Beneke, Jürgen; Lim, Joleen Chooi Hong; Sim, Chloe Pei-Ying; Chu, Yu‐Xia; Jiménez-Dalmaroni, Andrea; Joanny, J.-F.; Thiery, Jean Paul; Erfle, Holger; Parsons, Maddy; Mitchison, Timothy J.; Lim, Wendell A.; Lennon-Duménil, Ana María; Piel, Matthieu; Théry, Manuel

doi:10.1016/j.cub.2012.07.052

Cited by 143 publications

(135 citation statements)

References 7 publications

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“…Specifically, migration patterns normally ascribed to two or three dimensions such as fibrillar migration, capillary sprouting or lateral-line migration in the zebrafish embryo may be mimicked by the standardized 'one-dimensional' setting studied here [30,[56][57][58]. We predict a biphasic relationship between the probability of CIL and the emergence of collective cell migration (figure 6e): when CIL is either unlikely or very likely collective cell migration emerges very slowly, only intermediate CIL probabilities rapidly give rise to collective cell migration.…”

Section: Discussionmentioning

confidence: 99%

Contact inhibition of locomotion probabilities drive solitary versus collective cell migration

Desai

Gopal

Chen

et al. 2013

J. R. Soc. Interface.

123

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Contact inhibition of locomotion (CIL) is the process whereby cells collide, cease migrating in the direction of the collision, and repolarize their migration machinery away from the collision. Quantitative analysis of CIL has remained elusive because cell-to-cell collisions are infrequent in traditional cell culture. Moreover, whereas CIL predicts mutual cell repulsion and 'scattering' of cells, the same cells in vivo are observed to undergo CIL at some developmental times and collective cell migration at others. It remains unclear whether CIL is simply absent during collective cell migration, or if the two processes coexist and are perhaps even related. Here, we used micropatterned stripes of extracellular matrix to restrict cell migration to linear paths such that cells polarized in one of two directions and collisions between cells occurred frequently and consistently, permitting quantitative and unbiased analysis of CIL. Observing repolarization events in different contexts, including headto-head collision, head-to-tail collision, collision with an inert barrier, or no collision, and describing polarization as a two-state transition indicated that CIL occurs probabilistically, and most strongly upon head-to-head collisions. In addition to strong CIL, we also observed 'trains' of cells moving collectively with high persistence that appeared to emerge from single cells. To reconcile these seemingly conflicting observations of CIL and collective cell migration, we constructed an agent-based model to simulate our experiments. Our model quantitatively predicted the emergence of collective migration, and demonstrated the sensitivity of such emergence to the probability of CIL. Thus CIL and collective migration can coexist, and in fact a shift in CIL probabilities may underlie transitions between solitary cell migration and collective cell migration. Taken together, our data demonstrate the emergence of persistently polarized, collective cell movement arising from CIL between colliding cells.

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Section: Discussionmentioning

confidence: 99%

Contact inhibition of locomotion probabilities drive solitary versus collective cell migration

Desai

Gopal

Chen

et al. 2013

J. R. Soc. Interface.

123

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“…Human umbilical vein cell line EA.hy926 (American Type Culture Collection, Manassas, VA, USA; given by the Institute of Cancer Research London, UK; passage [10][11][12][13][14][15][16][17][18][19][20] were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Sigma) and 1% penicillin-streptomycin (Sigma). Cells were maintained under 5% CO 2 , 378C in a Binder (Germany) incubator.…”

Section: Live Cell Studies and Quantitative Analysismentioning

confidence: 99%

“…Based on these systems, Doyle et al [16] studied the fibroblast migration and revealed the rapid and uniaxial migratory behaviour of one-dimensional migration, mimicking the behaviour in a three-dimensional ECM. Similar one-dimensional systems have now been widely used for studying cellular motility and morphogenesis [17,18]. However, to our knowledge, detailed morphological characterizations of the dynamic interaction between the filopodial-like protrusion and a one-dimensional adhesive pattern are lacking.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of filopodium-like protrusion and endothelial cellular motility on one-dimensional extracellular matrix fibrils

et al. 2014

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Endothelial filopodia play key roles in guiding the tubular sprouting during angiogenesis. However, their dynamic morphological characteristics, with the associated implications in cell motility, have been subjected to limited investigations. In this work, the interaction between endothelial cells and extracellular matrix fibrils was recapitulated in vitro, where a specific focus was paid to derive the key morphological parameters to define the dynamics of filopodium-like protrusion during cell motility. Based on one-dimensional gelatin fibrils patterned by near-field electrospinning (NFES), we study the response of endothelial cells (EA.hy926) under normal culture or ROCK inhibition. It is shown that the behaviour of temporal protrusion length versus cell motility can be divided into distinct modes. Persistent migration was found to be one of the modes which permitted cell displacement for over 300 mm at a speed of approximately 1 mm min 21. ROCK inhibition resulted in abnormally long protrusions and diminished the persistent migration, but dramatically increased the speeds of protrusion extension and retraction. Finally, we also report the breakage of protrusion during cell motility, and examine its phenotypic behaviours.

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“…The kymographs can be further analyzed to extract position and size of cells as a function of time, allowing the study of different parameters to describe cell migration. Figure 5B shows the speed of migration tracked by imaging nucleus positioning using a homemade software 13 . DCs have a mean velocity close to 6 μm/min.…”

Section: Representative Resultsmentioning

confidence: 99%

Study of Cell Migration in Microfabricated Channels

Vargas

Terriac

Lennon-Duménil

et al. 2014

JoVE

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The method described here allows the study of cell migration under confinement in one dimension. It is based on the use of microfabricated channels, which impose a polarized phenotype to cells by physical constraints. Once inside channels, cells have only two possibilities: move forward or backward. This simplified migration in which directionality is restricted facilitates the automatic tracking of cells and the extraction of quantitative parameters to describe cell movement. These parameters include cell velocity, changes in direction, and pauses during motion. Microchannels are also compatible with the use of fluorescent markers and are therefore suitable to study localization of intracellular organelles and structures during cell migration at high resolution. Finally, the surface of the channels can be functionalized with different substrates, allowing the control of the adhesive properties of the channels or the study of haptotaxis. In summary, the system here described is intended to analyze the migration of large cell numbers in conditions in which both the geometry and the biochemical nature of the environment are controlled, facilitating the normalization and reproducibility of independent experiments. Video LinkThe video component of this article can be found at

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The first World Cell Race

Cited by 143 publications

References 7 publications

Contact inhibition of locomotion probabilities drive solitary versus collective cell migration

Contact inhibition of locomotion probabilities drive solitary versus collective cell migration

Dynamics of filopodium-like protrusion and endothelial cellular motility on one-dimensional extracellular matrix fibrils

Study of Cell Migration in Microfabricated Channels

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