The FLP proteins act as regulators of chlorophyll synthesis in response to light and plastid signals in <i>Chlamydomonas</i>

Falciatore, Angela; Merendino, Livia; Barneche, Frédy; Ceol, Mauro; Meskauskiene, Rasa; Apel, Klaus; Rochaix, Jean‐David

doi:10.1101/gad.321305

Cited by 63 publications

(54 citation statements)

References 45 publications

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“…RNAi of Alb3.2 can be induced by transferring these cells progressively to higher 5-FI concentrations. The lethality is not attributable to the RNAi system used, because another RNAi strain in which the FLP gene of Chlamydomonas was silenced could be maintained indefinitely under the same conditions (Falciatore et al, 2005). Therefore, Alb3.2 seems to have an essential function in the cell.…”

Section: Reduction Of Alb32 By Rnai Leads To a Decrease Of Psii And mentioning

confidence: 91%

“…The effect is attributable specifically to the depletion of Alb3.2, because the less affected strain Alb3.2_1 and another RNAi transformant targeted for FLP (Falciatore et al, 2005) did not display this phenotype (Figure 7). Measurements of the areas of vacuoles in 21 randomly chosen cell sections revealed that the vacuolar area represented 60 and 11% of the Wild-type cells were transformed with a plasmid carrying an inverted repeat of Alb3.2 cDNA using the cosilencing system described by Rohr et al (2004).…”

Section: Reduction Of Alb32 By Rnai Leads To a Decrease Of Psii And mentioning

confidence: 99%

“…To correct for possible effects of 5-FI on the accumulation of the photosynthetic proteins, the RNAi strain targeted for FLP and grown on 20 mM 5-FI was used as a control (Falciatore et al, 2005). With the exception of cytochrome f, VIPP1, and Cdj2, this strain accumulated the same amount of protein as the wild type.…”

Section: Reduction Of Alb32 By Rnai Leads To a Decrease Of Psii And mentioning

confidence: 99%

“…Serial dilutions from the FLP RNAi strain (control RNAi) (Falciatore et al, 2005) grown in the same conditions were used for quantification together with the wild type to correct for possible effects of 5-FI. Protein levels of Alb3.2_4 relative to the wild type are indicated at right.…”

Section: Reduction Of Alb32 By Rnai Leads To a Decrease Of Psii And mentioning

confidence: 99%

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One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas

et al. 2006

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Proteins of the YidC/Oxa1p/ALB3 family play an important role in inserting proteins into membranes of mitochondria, bacteria, and chloroplasts. In Chlamydomonas reinhardtii, one member of this family, Albino3.1 (Alb3.1), was previously shown to be mainly involved in the assembly of the light-harvesting complex. Here, we show that a second member, Alb3.2, is located in the thylakoid membrane, where it is associated with large molecular weight complexes. Coimmunoprecipitation experiments indicate that Alb3.2 interacts with Alb3.1 and the reaction center polypeptides of photosystem I and II as well as with VIPP1, which is involved in thylakoid formation. Moreover, depletion of Alb3.2 by RNA interference to 25 to 40% of wild-type levels leads to a reduction in photosystems I and II, indicating that the level of Alb3.2 is limiting for the assembly and/or maintenance of these complexes in the thylakoid membrane. Although the levels of several photosynthetic proteins are reduced under these conditions, other proteins are overproduced, such as VIPP1 and the chloroplast chaperone pair Hsp70/ Cdj2. These changes are accompanied by a large increase in vacuolar size and, after a prolonged period, by cell death. We conclude that Alb3.2 is required directly or indirectly, through its impact on thylakoid protein biogenesis, for cell survival.

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Section: Reduction Of Alb32 By Rnai Leads To a Decrease Of Psii And mentioning

confidence: 91%

Section: Reduction Of Alb32 By Rnai Leads To a Decrease Of Psii And mentioning

confidence: 99%

Section: Reduction Of Alb32 By Rnai Leads To a Decrease Of Psii And mentioning

confidence: 99%

Section: Reduction Of Alb32 By Rnai Leads To a Decrease Of Psii And mentioning

confidence: 99%

See 2 more Smart Citations

One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas

et al. 2006

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“…As such, it is likely that GLK gene function was required for chloroplast development in the last common ancestor of bryophytes and flowering plants. Therefore, GLK-mediated regulation of chloroplast development defines one of the most ancient conserved regulatory mechanisms identified in the plant kingdom, other notable examples being microRNA-mediated control of gene expression (Floyd and Bowman, 2004), regulation of state transitions in the chloroplast (Bellafiore et al, 2005), and protoporphyrin signaling from the chloroplast to the nucleus (Falciatore et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

A Conserved Transcription Factor Mediates Nuclear Control of Organelle Biogenesis in Anciently Diverged Land Plants

2005

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Land plant chloroplasts evolved from those found in the green algae. During land plant evolution, nuclear regulatory mechanisms have been modified to produce morphologically and functionally diverse chloroplasts in distinct developmental contexts. At least some of these mechanisms evolved independently in different plant lineages. In angiosperms, GOLDEN2-LIKE (GLK) transcription factors regulate the development of at least three chloroplast types. To determine whether GLK-mediated regulation of chloroplast development evolved within angiosperms or is a plesiomorphy within land plants, gene function was examined in the moss Physcomitrella patens. Gene expression patterns and loss-of-function mutant phenotypes suggested that GLK gene function is conserved between P. patens and Arabidopsis thaliana, species that diverged >400 million years ago. In support of this suggestion, moss genes partially complement Arabidopsis loss-of-function mutants. Therefore, GLK-mediated regulation of chloroplast development defines one of the most ancient conserved regulatory mechanisms identified in the plant kingdom.

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Biosynthesis of 5-Aminolevulinic Acid

Beale

Advances in Photosynthesis and Respiration

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The FLP proteins act as regulators of chlorophyll synthesis in response to light and plastid signals in Chlamydomonas

Abstract: [Keywords: Chlorophyll synthesis; plastid signal; light regulation; alternative splicing; Chlamydomonas] Supplemental material is available at http://www.genesdev.org.

Cited by 63 publications

References 45 publications

One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas

One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas

A Conserved Transcription Factor Mediates Nuclear Control of Organelle Biogenesis in Anciently Diverged Land Plants

Biosynthesis of 5-Aminolevulinic Acid

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