The follicular and endocrine environment in women with endometriosis: local and systemic cytokine production

Pellicer, A.; Albert, Carmela; Mercader, Amparo; Bonilla‐Musoles, Fernando; Remohı́, José; Simón, Carlos

doi:10.1016/s0015-0282(98)00204-0

Cited by 183 publications

(140 citation statements)

References 24 publications

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“…Several studies demonstrate that intrafollicular levels of cytokines are altered in patients with endometriosis [9,20,21]. Elevated levels of cytokines in serum and peritoneal fluid of endometriosis cases are also reported [22,23].…”

Section: Discussionmentioning

confidence: 99%

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Singh

Dutta

Chattopadhyay

et al. 2016

J Assist Reprod Genet

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Purpose The study aimed to investigate key intrafollicular prognostic factors among various cytokines and angiogenic molecules for prediction of mature oocytes and good-quality embryos in women with endometriosis undergoing in vitro fertilization (IVF). Methods Paired follicular fluid and serum samples were collected from 200 women with advanced stage endometriosis and 140 normal ovulating women during oocyte retrieval. The concentrations of cytokines (pro-inflammatory: IL-1β, TNF-α, IL-2, IL-8, IL-12, IFN-γ; anti-inflammatory: IL-4, IL-6, IL-10) and angiogenic molecules (vascular endothelial growth factor (VEGF), adrenomedullin, angiogenin) were determined in follicular fluid and serum using ELISA. Expression of these molecules was subjected to multivariate analysis for the identification of major predictive markers of oocyte and embryo quality. Receiver operating characteristic (ROC) curve was applied to determine the best cutoff point for the discrimination between mature and immature oocytes in these women. Results Significant increases in levels of cytokines and angiogenic molecules were observed in women with endometriosis compared to controls (P < 0.001). From the validated partial least squares-discriminant analysis (PLS-DA) model, IL-8, IL-12, and adrenomedullin were identified as the most important factors contributing to endometriosis and were negatively associated with oocyte maturity and embryo quality. Conclusion The levels of IL-8, IL-12, and adrenomedullin may be good indicators of embryo and oocyte quality in endometriosis patients undergoing IVF. Further studies are necessary to ascertain the potential of these markers for oocyte and embryo developmental competence which may help improve the chances of a successful IVF in endometriosis patients.

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Section: Discussionmentioning

confidence: 99%

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Singh

Dutta

Chattopadhyay

et al. 2016

J Assist Reprod Genet

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show abstract

“…Several studies have found increased levels of inflammatory cytokines such as interleukin (IL)-1β [5], IL-6 [6,7], IL-8 [8] and IL-18 [9] in the peritoneal fluid [5,8,9] and in the circulation [6,7] in patients with endometriosis.…”

Section: Introductionmentioning

confidence: 99%

“…Most but not all [6,8,10] studies revealed increased intrafollicular levels of cytokines such as IL-1β, IL-6 [6] and IL-8 [8]. In addition, previous reports have demonstrated that the hormonal milieu was altered in the follicular fluid of patients with endometriosis, such as a decreased estradiol concentration [2].…”

Section: Introductionmentioning

confidence: 99%

Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis

Bersinger²,

Mueller³

et al. 2017

J Assist Reprod Genet

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Purpose The aim of this study was to assess whether the intrafollicular cytokine profile in naturally developed follicles is different in women with endometriosis, possibly explaining the lower reproductive outcome in endometriosis patients. Methods A matched case-control study was conducted at a university-based infertility and endometriosis centre. The study population included 17 patients with laparoscopically and histologically confirmed endometriosis (rAFS stages II-IV), each undergoing one natural cycle IVF (NC-IVF) treatment cycle between 2013 and 2015, and 17 age-matched NC-IVF women without diagnosed endometriosis (control group). Follicular fluid and serum was collected at the time of follicle aspiration. The concentrations of inflammatory cytokines (IL-1β, IL-6, IL-8, IL-15, IL-18, TNF-α) and hormones (testosterone, estradiol, AMH) were determined in follicular fluid and serum by single or multiplexed immunoassay and compared between both groups. Results In the follicular fluid, IL-1β and IL-6 showed significantly (P < 0.001 and 0.01, respectively) higher median concentrations in the endometriosis group than in the control group and a tendency towards endometriosis severity (rAFS stage) dependence. The levels of the interleukins detectable in follicular fluid were significantly higher than those in the serum (P < 0.01). Follicular estradiol concentration was lower in severe endometriosis patients than in the control group (P = 0.036). Follicular fluid IL-1β and IL-6 levels were not correlated with estradiol in the same compartment in neither patient group. Conclusions In women with moderate and severe endometrioses, some intrafollicular inflammatory cytokines are upregulated and not correlated with intrafollicular hormone concentrations. This might be due to the inflammatory microenvironment in endometriosis women, affecting follicular function and thereby possibly contributing to the reproductive dysfunction in endometriosis.

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“…Unexplained infertility is a term that has been applied to as many as 30-40% of infertile couples (Smith et al, 2003). The potential causes of unexplained infertility have been described as disturbance in endocrinological balance, immunology, genetic and reproductive physiology (Pellicer et al, 1998). The proportion of couples suffering from unexplained infertility is popularly quoted as 16% but the range varies from 0% to 37%.…”

Section: …………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%

Cytogenetic Studies of Couples With Idiopathic Infertility.

P¹,

Reshmi²,

Breezevilla³

et al. 2017

IJAR

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The follicular and endocrine environment in women with endometriosis: local and systemic cytokine production

Cited by 183 publications

References 24 publications

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis

Cytogenetic Studies of Couples With Idiopathic Infertility.

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