The function of the m6A methyltransferase METTL3 in goat early embryo development under hypoxic and normoxic conditions

Li, Dongxu; Liu, Zifei; Deng, Mingtian; Lang, Liang; Lu, Jiawei; Wang, Zhen; Wan, Yongjie

doi:10.1016/j.theriogenology.2021.10.017

Cited by 6 publications

(2 citation statements)

References 51 publications

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“…Studies have found that m 6 A contributes to a variety of biological processes and plays different roles across these different biological processes. For example, the role of m 6 A in development has been demonstrated in various organisms, such as humans [26] , mice [27] , goats [28] , pigs [29] , and bees [30] . Specifically, the importance of m 6 A in the development of different organs, such as the heart [31] , gonads [32] , testes [33] , brain [34] , and cerebellum [35] , is gradually being revealed.…”

Section: Basic Knowledge Of M 6 a Methylationmentioning

confidence: 99%

m<sup>6</sup>A methylation in cellular senescence of age-associated diseases

Gao¹,

Yao²,

Pang³

et al. 2023

ABBS

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Cellular senescence is a state of irreversible cellular growth arrest that occurs in response to various stresses. In addition to exiting the cell cycle, senescent cells undergo many phenotypic alterations, including metabolic reprogramming, chromatin rearrangement, and senescence-associated secretory phenotype (SASP) development. Furthermore, senescent cells can affect most physiological and pathological processes, such as physiological development; tissue homeostasis; tumour regression; and age-associated disease progression, including diabetes, atherosclerosis, Alzheimer’s disease, and hypertension. Although corresponding anti-senescence therapies are actively being explored for the treatment of age-associated diseases, the specific regulatory mechanisms of senescence remain unclear. N 6 -methyladenosine (m 6 A), a chemical modification commonly distributed in eukaryotic RNA, plays an important role in biological processes such as translation, shearing, and RNA transcription. Numerous studies have shown that m 6 A plays an important regulatory role in cellular senescence and aging-related disease. In this review, we systematically summarize the role of m 6 A modifications in cellular senescence with regard to oxidative stress, DNA damage, telomere alterations, and SASP development. Additionally, diabetes, atherosclerosis, and Alzheimer’s disease regulation via m 6 A-mediated cellular senescence is discussed. We further discuss the challenges and prospects of m 6 A in cellular senescence and age-associated diseases with the aim of providing rational strategies for the treatment of these age-associated diseases.

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Section: Basic Knowledge Of M 6 a Methylationmentioning

confidence: 99%

m<sup>6</sup>A methylation in cellular senescence of age-associated diseases

Gao¹,

Yao²,

Pang³

et al. 2023

ABBS

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“…Notably, in mouse uterus, both Mettl3 and m6A levels were increasing as pregnancy progressed [21]. METTL3 is critical for goat ZGA [22]; however, the levels and function of METTL3 and m6A in goat uterus during early pregnancy have not been reported. Moreover, previously, we found that connective tissue growth factor ( CTGF ) was highly expressed in goat endometrium during early pregnancy [23].…”

Section: Introductionmentioning

confidence: 99%

METTL3 promotes proliferation of goat endometrial epithelial cells by regulating CTGF in an m6A-dependent manner

Sun

Zhang

et al. 2023

Biology of Reproduction

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N6-methyladenosine (m6A), an epigenetic modification on RNAs, plays an important role in many physiological and pathological processes. However, the involvement of m6A in goat uterus during early pregnancy remains largely unknown. In this study, we found that the total m6A level was increasing in goat uterus as early pregnancy progressed. Methyltransferase-like 3 (METTL3) is a core catalytic subunit of the m6A methyltransferase. We thus determined the expression and regulation of METTL3 in goat uterus. METTL3 was highly expressed in the luminal and glandular epithelia from day 16 (D16) to D25 of pregnancy. And it could be up-regulated by estrogen and progesterone in goat uterus and primary endometrial epithelial cells (EECs). In EECs, knockdown or overexpression of METTL3 resulted in a significant decrease or increase of cell proliferation, respectively. METTL3 knockdown reduced the m6A level of not only total RNA but also connective tissue growth factor (CTGF) mRNA. Luciferase assay suggested that METTL3 might target the potential m6A sites in the 3’untranslated region (3’UTR) of CTGF mRNA. Moreover, METTL3 positively regulated CTGF expression, and CTGF knockdown significantly counteracted the promoting effect of METTL3 overexpression on EEC proliferation. Collectively, METTL3 is dynamically expressed in goat uterus and can affect EEC proliferation by regulating CTGF in an m6A-dependent manner. Our results will lay a foundation for further studying the crucial mechanism of METTL3-mediated m6A modification in goat uterus during early pregnancy.

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Antioxidant supplementation may effect DNA methylation patterns, apoptosis, and ROS levels in developing mouse embryos

Uysal,

Sukur,

Bozdemir

et al. 2024

Histochem Cell Biol

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This study was designed to address the question: does antioxidant-containing embryo culture media affect DNA methyltransferases, global DNA methylation, inner cell mass/trophoblast differentiation, intracellular reactive oxygen species (ROS) levels, and apoptosis? Mouse zygotes were cultured in embryo culture media containing MitoQ, N-acetyl-l-cysteine (NAC), acetyl-l-carnitine (ALC), α-lipoic acid (ALA), or the mixture of NAC + ALC + ALA (AO) until the blastocyst stage, whereas in vivo-developed blastocysts were used as control. Protein expression levels of Dnmt1, 3a, 3b, and 3l enzymes were analyzed by immunofluorescence and western blot, while global DNA methylation, apoptosis, and ROS levels were evaluated by immunofluorescence. NAC, ALC, and MitoQ significantly increased the levels of all Dnmts and global methylation. ALA significantly induced all Dnmts, whereas global methylation did not show any difference. NAC and mixture AO applications significantly induced Nanog levels, ALA and MitoQ increased Cdx2 levels, while the other groups were similar. ALA and MitoQ decreased while ALC increased the levels of intracellular ROS. This study illustrates that antioxidants, operating through distinct pathways, have varying impacts on DNA methylation levels and cell differentiation in mouse embryos. Further investigations are warranted to assess the implications of these alterations on the subsequent offspring.

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The function of the m6A methyltransferase METTL3 in goat early embryo development under hypoxic and normoxic conditions

Cited by 6 publications

References 51 publications

m<sup>6</sup>A methylation in cellular senescence of age-associated diseases

m<sup>6</sup>A methylation in cellular senescence of age-associated diseases

METTL3 promotes proliferation of goat endometrial epithelial cells by regulating CTGF in an m6A-dependent manner

Antioxidant supplementation may effect DNA methylation patterns, apoptosis, and ROS levels in developing mouse embryos

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The function of the m6A methyltransferase METTL3 in goat early embryo development under hypoxic and normoxic conditions

Cited by 6 publications

References 51 publications

m&lt;sup&gt;6&lt;/sup&gt;A methylation in cellular senescence of age-associated diseases

m&lt;sup&gt;6&lt;/sup&gt;A methylation in cellular senescence of age-associated diseases

METTL3 promotes proliferation of goat endometrial epithelial cells by regulating CTGF in an m6A-dependent manner

Antioxidant supplementation may effect DNA methylation patterns, apoptosis, and ROS levels in developing mouse embryos

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m<sup>6</sup>A methylation in cellular senescence of age-associated diseases

m<sup>6</sup>A methylation in cellular senescence of age-associated diseases